DPPE-PEG5000  (Synonyms: 16:0 PEG5000 PE; 1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-5000] ammonium)

DPPE-PEG5000 (16:0 PEG5000 PE) is a polymer-lipid conjugate and LipoParticle stabilizer with a PEG chain of 5,000 g/mol molecular weight attached to its polar head, and it can be internalized by biological membranes. DPPE-PEG5000 enables LipoParticle to maintain colloidal stability after 20-fold dilution in PBS or cell culture medium, and prevents aggregate formation during lyophilization and rehydration. DPPE-PEG5000 helps enhance the non-cytotoxic property of LipoParticle formulations against human osteoblasts. DPPE-PEG5000 serves as a PEG lipid functional end group for synthesizing liposomes (LPs), is used in the design of conjugated polymer nanoparticles, and applies to research related to bone and joint infections^[1].

DPPE-PEG5000 (2 mol./mol. total lipid) imparts colloidal stability to LipoParticles upon 20-fold dilution in PBS or cell culture medium and enables freeze-drying and rehydration without significant aggregation, while maintaining defined size, size distribution, and surface charge profiles^[1].
DPPE-PEG5000 (2 mol./mol. total lipid; 19.5-1250 μg/mL LipoParticles; 24 h) forms LipoParticles that are non-cytotoxic to human osteoblast MG63 cells at concentrations up to 1250 μg/mL after 24 h of incubation, with no reduction in cell metabolic activity^[1].
DPPE-PEG5000 (2 mol./mol. total lipid; 3,000 colloids per cell; 24 h) forms LipoParticles that are efficiently internalized into human osteoblast MG63 cells after 24 h of incubation at a dose of 3,000 colloids per cell, with localization in the cellular cytoplasm^[1].
DPPE-PEG5000 (2 mol./mol. total lipid; 22,000 colloids per cell; 24 h) forms LipoParticles that are internalized into human osteoblast MG63 cells after 24 h of incubation at a dose of 22,000 colloids per cell, with measurable fluorescence signals from both the PLGA core and lipid coating, and maintain high cell viability (89.7%)^[1].
DPPE-PEG5000 (2 mol./mol. total lipid; 22,000 colloids per infected cell; 24 h) forms LipoParticles that are internalized into Staphylococcus aureus-infected human osteoblast MG63 cells after 24 h of incubation at a dose of 22,000 colloids per cell, with localization near intracellular bacteria^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

5000(Average)

(C₂H₄O)_nC₃₉H₇₆NO₁₀P.H₃N

474922-84-4

Solid

White to off-white

O=C(NCCOP(O)(OC[C@@H](COC(CCCCCCCCCCCCCCC)=O)OC(CCCCCCCCCCCCCCC)=O)=O)OCCOC.N.[n]

Room temperature in continental US; may vary elsewhere.

• [1]. Vanneste F, et al. LipoParticles: a lipid membrane coating onto polymer particles to enhance the internalization in osteoblast cells. Nanoscale. 2023;15(44):18015-18032. Published 2023 Nov 16.  [Content Brief]