Biotin-11-dCTP
Based on 1 Customer Validation
Biotin-11-dCTP is a biotinylated deoxynucleoside triphosphate and an important DNA labeling reagent. In random primer DNA labeling reactions, Biotin-11-dCTP incorporates into newly synthesized DNA strands to generate labeled DNA probes suitable for hybridization applications. In addition, Biotin-11-dCTP can serve as a substrate for terminal deoxynucleotidyl transferase to end-label oligonucleotides for telomere sequence detection, or to label the cut ends of linearized DNA molecules, thereby supporting streptavidin-based electron microscopy analysis. For example, Biotin-11-dCTP can label the cut ends of linearized DNA molecules under the action of dGTP and avian myeloblastosis virus reverse transcriptase.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.1%
- 分子式: C28H43Li3N7O16P3S
- 分子量:879.49
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保管条件:
Solution, -20°C, protect from light, 2 years
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生物活性
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化学情報
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
Biotin-11-dCTP (200-fold excess over oligomer 3' ends) enables effective end-labeling of (GGGTTA)7 and (TAACCC)7 oligomers, which then hybridize to telomeric sequences in human, Chinese hamster, and rat chromosomes[1].
Biotin-11-dCTP (0.02 mM; 45 min at 37°C) effectively labels linearized hemimethylated circular G-T heteroduplex DNA termini to enable mapping of mismatch repair excision tracts via electron microscopy[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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性状 Liquid
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分子量 879.49
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分子式 C28H43Li3N7O16P3S
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Color Colorless to light yellow
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SMILES
[H][C@@]1(NC(N2)=O)[C@@]2([H])[C@H](CCCCC(NCCCCCC(NCC#CC(C(N)N3)=CN([C@H]4C[C@H](O)[C@@H](COP(OP(OP(O)(O[Li])=O)(O[Li])=O)(O[Li])=O)O4)C3=O)=O)=O)SC1
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Solution, -20°C, protect from light, 2 years
純度とドキュメンテーション
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データシート (266 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Moyzis RK, et al. A highly conserved repetitive DNA sequence, (TTAGGG)n, present at the telomeres of human chromosomes. Proc Natl Acad Sci U S A. 1988;85(18):6622-6626. [Content Brief]
[2]. Grilley M, et al. Bidirectional excision in methyl-directed mismatch repair. J Biol Chem. 1993;268(16):11830-11837. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Biotin-11-dCTP 関連分類
製品番号: HY-D1668
Powered by Bioz
- Biotin-11-dCTP
- DNA Stain
- Reverse Transcriptase
- DNA labeling agent
- avian myeloblastosis virus reverse transcriptase
- random-primed DNA labeling reactions
- cytosine
- terminal deoxynucleotidyltransferase
- telomeric sequences
- DNA probes
- metaphase chromosomes
- DNA strands
- deoxynucleoside triphosphate
- Inhibitor
- inhibitor
- inhibit