Biotin TSA(200×)
Based on 8 publication(s) in Google Scholar
Biotin TSA (200×) (Biotin Tyramide) is a biotin derivative used for tyramide signal amplification (TSA), as a reagent to amplify both immunohistochemical signals and in situ hybridization protocols. Biotinyl tyramide can be used for the research of tyramide signal amplification.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
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보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications Citing Use of MedChemExpress (MCE) Biotin TSA(200×)
More- Nature. 2025 May;641(8061):202-210. [Abstract]
- Nat Immunol. 2025 Mar;26(3):391-403. [Abstract]
- Nat Cell Biol. 2022 Apr;24(4):497-512. [Abstract]
- Nat Commun. 2023 Apr 25;14(1):2378. [Abstract]
- Genome Biol. 2022 Dec 15;23(1):259. [Abstract]
- Free Radic Biol Med. 2026 Mar 16:246:69-79. [Abstract]
- bioRxiv. 2026 May 21.
- Med Rev (2021). 2024 Jun 14;4(6):531-543. [Abstract]
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Biological Activity
Biotinyl tyramide (100 μM, 5min) and hemin (HY-19424) enhance biotinylation of starved U2OS cells after validating the G4-specific biotinylation activity of RNA-hemin complexes in vitro[6].
Biotinyl tyramide (27.5 μM, 5min) binds to the tyrosine side chains of cell surface proteins in HaCaT wild-type cells or dual oxidase 1 (DUOX1) knockout cells[7].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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SMILES
[Biotin TSA(200×)]
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Synonyms
Biotin Tyramide
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (8)
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Journal Impact Factor
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Most Recent
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Nature
2025 May;641(8061):202-210. PMID: 40140567
Biotin TSA(200×) purchased from MedChemExpress. Usage Cited in: Nature. 2025 May;641(8061):202-210. [Abstract]
HEK293T cells expressing STING-APEX2 were first treated with 0.5 μM diBAZI, OVA peptide, or ABM5-OVA for 2 h, then with Biotinylated tyramide (500 μM; 30 min) and H2O2. Biotinylated proteins were pulled down by streptavidin magnetic beads and analyzed by western blot for TAP1 and proteasomes.
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Nat Immunol
The lactate receptor HCAR1 drives the recruitment of immunosuppressive PMN-MDSCs in colorectal cancer. [Abstract]2025 Mar;26(3):391-403. PMID: 39905201 -
Nat Cell Biol
2022 Apr;24(4):497-512. PMID: 35332264 -
Nat Commun
Isthmin-1 (Ism1) modulates renal branching morphogenesis and mesenchyme condensation during early kidney development. [Abstract]2023 Apr 25;14(1):2378. PMID: 37185772
Biotin TSA(200×) purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Apr 25;14(1):2378. [Abstract]
Proximity labelling of peroxidase-based approach. Biotin-phenol (Biotinyl tyramide, 500 μM; 30 min) was oxidized into reactive phenoxyl radicals with hydrogen peroxide existence, thus enabling the proximal labelling. Biotinylated proteins from a proximity labeling experiment analyzed by biotin and streptavidin-HRP blot in E11.5 wild-type kidney rudiments. Clear changes in band pattern were observed for HRP-Flag-Ism1 treated samples compared with ligand-free samples. WCL, whole cell lysis.
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Genome Biol
G-quadruplexes sense natural porphyrin metabolites for regulation of gene transcription and chromatin landscapes. [Abstract]2022 Dec 15;23(1):259. PMID: 36522639 -
Free Radic Biol Med
Mitochondrial cysteinyl-tRNA synthetase 2 protects against excitotoxic retinal cell death via enhanced supersulfide production. [Abstract]2026 Mar 16:246:69-79. PMID: 41506554 -
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Med Rev (2021)
2024 Jun 14;4(6):531-543. PMID: 39664081
Biotin TSA(200×) purchased from MedChemExpress. Usage Cited in: Med Rev (2021). 2024 Jun 14;4(6):531-543. [Abstract]
Biotinyl tyramide (500 μmol/L; 30 min). Immunofluorescence-validated APEX2-MST1 labeling specificity in KYSE150 cells, biotin red, MST1 green; right: expression of the APEX2-MST1 fusion protein was observed.
Biotin TSA(200×) purchased from MedChemExpress. Usage Cited in: Med Rev (2021). 2024 Jun 14;4(6):531-543. [Abstract]
Biotinyl tyramide (500 μmol/L; 30 min) was used. Streptavidin-HRP Western blotting was performed to detect induced protein biotinylation in lysates from cells expressing APEX2-MST1.
순도&문서
References
[1]. Evans M F, et al. Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens [J]. BMC Clinical Pathology, 2003, 3: 1-17. [Content Brief]
[2]. Kharel P, et al. Stress promotes RNA G-quadruplex folding in human cells [J]. Nature Communications, 2023, 14(1): 205. [Content Brief]
[3]. Bobrow M N, et al. Catalyzed reporter deposition, a novel method of signal amplification application to immunoassays [J]. Journal of immunological methods, 1989, 125(1-2): 279-285. [Content Brief]
[4]. Pató A, et al. Hydrogen peroxide production by epidermal dual oxidase 1 regulates nociceptive sensory signals [J]. Redox Biology, 2023, 62: 102670. [Content Brief]
[5]. Kim S H, et al. An improved protocol of biotinylated tyramine-based immunohistochemistry minimizing nonspecific background staining [J]. Journal of Histochemistry & Cytochemistry, 2003, 51(1): 129-132. [Content Brief]
[6]. Dráberová E, et al. Quantification of α-tubulin isotypes by sandwich ELISA with signal amplification through biotinyl-tyramide or immuno-PCR [J]. Journal of immunological methods, 2013, 395(1-2): 63-70. [Content Brief]
[7]. Hunyady B, et al. Immunohistochemical signal amplification by catalyzed reporter deposition and its application in double immunostaining [J]. Journal of Histochemistry & Cytochemistry, 1996, 44(12): 1353-1362. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)