CHI3L1-IN-3
Based on 1 Customer Validation
CHI3L1-IN-3 is a CHI3L1 inhibitor. CHI3L1-IN-3 binds to CHI3L1 with Kds of 13.76 μM and 13.5 μM in MST and SPR assays, respectively. CHI3L1-IN-3 demonstrates extended plasma half-lives and microsomal stability, along with reduced intrinsic clearance. CHI3L1-IN-3 induces dose-dependent cytotoxicity, reduces spheroid mass and inhibits migration in a 3D multicellular glioblastoma (GBM) spheroid model. CHI3L1-IN-3 can be used for the study of GBM.
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- Purity: 98.95%
- CAS No.: 3109469-27-1
- 화학식: C30H33N3O6S
- 분자량:563.66
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보관:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
CHI3L1-IN-3 (Compound 11g) exhibits enhanced stability, with increased plasma half-lives of 2.0 h (mouse) and 2.5 h (human), and microsomal half-lives of 2.2 h (mouse) and 2.8 h (human) and lower clearance rates of 15 mL/min/mg (mouse) and 19 mL/min/mg (human)[1]. CHI3L1-IN-3 (50 μM, 72 h) demonstrates low cytotoxicity in hBMECs and HepG2 cells and no cytotoxicity in NHA cells[1]. CHI3L1-IN-3 (5-25 μM, 72 h) shows a dose-dependent reduction in cell viability of GBM spheroids[1]. CHI3L1-IN-3 (10 μM, 72 h) reduces spheroid weight by over 50 %, consistent with widespread cell death and loss of structural cohesion[1]. CHI3L1-IN-3 (10 μM, 24-96 h) suppresses GBM spheroids migration by 60 %[1]. CHI3L1-IN-3 exhibits antiproliferative activity in glioblastoma cell lines, with IC50 values of 2.19 μM in U251 MG and 5.06 μM in T98G cells[1]. CHI3L1-IN-3 (5-25 μM, 72 h) reduces pSTAT3 levels dose-dependently in GBM spheroids[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:GBM spheroids
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Concentration:5, 10, 25 μM
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Incubation Time:72 h
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Result:Showed a dose-dependent reduction in cell viability of GBM spheroids.
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Cell Line:GBM spheroids
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Concentration:5, 10, 25 μM
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Incubation Time:24, 48, 72, 96 h
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Result:Suppressed GBM spheroids migration by 60 %.
Chemical Information
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CAS No. 3109469-27-1
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Appearance Solid
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분자량 563.66
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화학식 C30H33N3O6S
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Color Off-white to light yellow
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SMILES
COC(C(OC)=C1)=CC(C2=O)=C1N=C(SCC(NC3=CC=C(CC)C=C3)=O)N2CCC4=CC=C(OC)C(OC)=C4
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
용액&용해도
DMSO : 50 mg/mL (88.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (277 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.7741 mL | 8.8706 mL | 17.7412 mL | 44.3530 mL |
| 5 mM | 0.3548 mL | 1.7741 mL | 3.5482 mL | 8.8706 mL | |
| 10 mM | 0.1774 mL | 0.8871 mL | 1.7741 mL | 4.4353 mL | |
| 15 mM | 0.1183 mL | 0.5914 mL | 1.1827 mL | 2.9569 mL | |
| 20 mM | 0.0887 mL | 0.4435 mL | 0.8871 mL | 2.2176 mL | |
| 25 mM | 0.0710 mL | 0.3548 mL | 0.7096 mL | 1.7741 mL | |
| 30 mM | 0.0591 mL | 0.2957 mL | 0.5914 mL | 1.4784 mL | |
| 40 mM | 0.0444 mL | 0.2218 mL | 0.4435 mL | 1.1088 mL | |
| 50 mM | 0.0355 mL | 0.1774 mL | 0.3548 mL | 0.8871 mL | |
| 60 mM | 0.0296 mL | 0.1478 mL | 0.2957 mL | 0.7392 mL | |
| 80 mM | 0.0222 mL | 0.1109 mL | 0.2218 mL | 0.5544 mL |
CHI3L1-IN-3 Related Classifications
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