IL-20R alpha Protein, Mouse (HEK293, Fc)
Based on 1 Customer Validation
IL-20R alpha Protein (IL20RA) is the alpha subunit of IL-20 receptor, forms functional heterodimers with different subunit protein and involves in STAT3 activation pathway. The IL20RA/IL20RB dimer is a receptor for IL-19, IL-20 and IL-24, while the IL20RA/IL10RB dimer is a receptor for IL-26. IL-20R alpha Protein is consists of 553 amino acids (M1-N553) with a transmembrane domain (254-274 a.a.). IL-20R alpha Protein, Mouse (HEK293, Fc) is produced in HEK293 cells with a C-Terminal His-tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
IL-20R alpha Protein (IL20RA) is the alpha subunit of IL-20 receptor, forms functional heterodimers with different subunit protein and involves in STAT3 activation pathway[1]. The IL20RA/IL20RB dimer is a receptor for IL-19, IL-20 and IL-24, while the IL20RA/IL10RB dimer is a receptor for IL-26[3]. IL-20R alpha Protein is consists of 553 amino acids (M1-N553) with a transmembrane domain (254-274 a.a.). IL-20R alpha Protein, Mouse (HEK293, Fc) is produced in HEK293 cells with a C-Terminal His-tag.
IL-20R alpha, also known as a alpha subunit of IL-20 receptor (IL-20RA), belongs to the type II cytokine receptor family[1].
IL-20R alpha is widely expressed with highest levels in skin and testis and high levels in brain[2].
IL20RA and IL20RB is found to be upregulated in psoriatic skin lesions on keratinocytes[3].
IL-20R alpha forms functional heterodimers with different subunit protein. IL-20R alpha serves as the receptor for IL-19, IL-20 and IL-24 by dimerizing with IL20RB, however serves as the receptor for IL-26 by dimerizing with IL-10RB. IL-20R alpha transduces ligand-binding at least in part through STAT3 activation, and plays an important role in epidermal function and psoriasis[4].
The sequence of amino acids in IL-12 beta proteins of mouse is very different from human (64.64%).
Overexpression of IL-20 in transgenic (TG) mice causes neonatal lethality with skin abnormalities including aberrant epidermal differentiation[1].
Mouse IL-20RA, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-20, His Tag with an affinity constant of 13.93 μM as determined in SPR assay (Biacore T200).
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-hFc
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Accession
Q6PHB0 (V33-K253)
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Molecular Construction
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N-term
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IL-20Rα (V33-K253)
Accession # Q6PHB0 -
hFc
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C-term
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Protein Length
Extracellular Domain
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Synonyms
IL-20R-alpha; IL-20RA; CRF2-8; IL-20R1; ZcytoR7; FLJ40993
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AA Sequence
VPCVFCGLPKPTNITFLSINMKNVLHWNPPESLHGVEVTYTVQYFIYGQKKWLNASKCGSINRTYCDLSVETSDYEHQFYAKVKAIWEARCSEWAETERFYPFLETQVSPPEVALTTGEKSISIALTAPEKWKRNPQDHTVSMQQIYPNLKYNVSVYNTKSRRTWSQCVTNSTLVLSWLEPNTLYCVHVESLVPGPPRLPMPSQKQCISTLEVQTSAWKAK
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Predicted Molecular Mass
52.1 kDa
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Molecular Weight
Approximately 65-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Wang F, et al. Prominent production of IL-20 by CD68+/CD11c+ myeloid-derived cells in psoriasis: Gene regulation and cellular effects. J Invest Dermatol. 2006 Jul;126(7):1590-9. [Content Brief]
[2]. Blumberg H, et al. Interleukin 20: discovery, receptor identification, and role in epidermal function. Cell. 2001 Jan 12;104(1):9-19. [Content Brief]
[3]. Sheikh F, et al. Cutting edge: IL-26 signals through a novel receptor complex composed of IL-20 receptor 1 and IL-10 receptor 2. J Immunol. 2004 Feb 15;172(4):2006-10. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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