Lichenase, Microorganism
Based on 1 Customer Validation
Lichenase, Microorganism (endo-1,3:1,4-β-D-Glucanase) is a specific, endo-(1-3),(1-4)-β-D-glucan 4-glucanohydrolase. Lichenase, Microorganism solubilizes β-glucans from cereal grains and gives gluco-oligosaccharides (GOS). Lichenase, Microorganism can be used in the degradation of polysaccharides in the cell walls.
For research use only. We do not sell to patients.
- CAS No.: 37288-51-0
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All Endogenous Metabolite Isoforms
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Biological Activity
Product Information
EDTA, Zn2+ and Fe3+ may inhibit enzyme activity.
Hydrolysis properties of the lichenase
1. Incubate 1% β-glucan (20 mm Na2HPO4-citrate, pH 6.5) with 10 U Lichenase at 60°C and stir at 150 rpm.
2. Remove aliquots periodically and place the samples in boiling water for 10 min to stop the reaction.
3. The enzymatic solution was treated with 10% isopropanol and centrifuged at 10,000 rpm for 15 min to remove the residue.
4. Take the supernatant (2 μL) and perform thin layer chromatography analysis using a butanol acetic acid-water (2:1:1, v/v) solvent system.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
3.2.1.6
≥1000 U/mL
One unit is defined as the amount of enzyme that is required to produce 1 μmol of reducing sugar (calculated as glucose) per minute under the assay conditions
Chemical Information
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CAS No. 37288-51-0
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[Lichenase, Microorganism]
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Synonyms
endo-1,3:1,4-β-D-Glucanase
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
DMSO : 100 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Purity & Documentation
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Data Sheet (267 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Huang Z, et al. Characterization of a Thermostable Lichenase from Bacillus subtilis B110 and Its Effects on β-Glucan Hydrolysis. J Microbiol Biotechnol. 2022 Apr 28;32(4):484-492. [Content Brief]
[2]. Tyurin AA, et al. A high throughput assay of lichenase activity with Congo red dye in plants. Plant Methods. 2021 Oct 9;17(1):102. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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