2F-Peracetyl-Fucose
Based on 3 publication(s) in Google Scholar
2F-Peracetyl-Fucose (1,3,4-Tri-O-acetyl-2-deoxy-2-fluoro-L-fucopyranos) acts as a potent fucosyltransferase (FUT) inhibitor.
For research use only. We do not sell to patients.
- Purity: 99.54%
- CAS No.: 188783-78-0
- Formula: C12H17FO7
- Molecular Weight:292.26
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) 2F-Peracetyl-Fucose
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Customer Validation & Images
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Cell Imaging/Staining
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Cell Migration/Invasion Assay
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IF
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WB
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RT-PCR
Biological Activity
2F-Peracetyl-Fucose (64 μM, 72 hours) inhibits orosphere formation and invasion in UMSCC14B and UMSCC103 cells[2]. 2F-PAF (25-200 μM, 48 hours) inhibits migration ability in NCI-H3122 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:UMSCC14B, UMSCC103 (head and neck squamous cell carcinoma)
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Concentration:64 μM
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Incubation Time:72 hours
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Result:Treated spheres were smaller with reduced invasion.
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Cell Line:NCI-H3122 (NSCLC)
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Concentration:25-200 μM
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Incubation Time:48 hours
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Result:Reduced number of migrated cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Calu-1 xenograft BALB/c nude mouse model[3]
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Dosage:20 μg/ml
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Administration:Intravenous injection (i.v.), single dose
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Result:Reduced tumor colonization ability in the lungs.
Chemical Information
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CAS No. 188783-78-0
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Appearance Solid
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Molecular Weight 292.26
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Formula C12H17FO7
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Color White to off-white
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SMILES
C[C@H]1[C@@H](OC(C)=O)[C@@H](OC(C)=O)[C@H](F)C(OC(C)=O)O1
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Synonyms
1,3,4-Tri-O-acetyl-2-deoxy-2-fluoro-L-fucopyranos
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (3)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
Synthetic Retinoid Sulfarotene Selectively Inhibits Tumor-Repopulating Cells of Intrahepatic Cholangiocarcinoma via Disrupting Cytoskeleton by P-Selectin/PSGL1 N-Glycosylation Blockage. [Abstract]2024 Nov 28:e2407519. PMID: 39605300
2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Nov 28:e2407519. [Abstract]
Inhibition of 2F-Peracetyl-Fucose (2‐FF) (10-50 μM) on colony spheroid growth in HUCCT1‐TRCs and RBE‐TRCs. The representative images of colony spheroids on day 8 (treatment from day3). 2‐FF treatment significantly inhibited the growth of colony spheroids.
2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Nov 28:e2407519. [Abstract]
Effects of 2F-Peracetyl-Fucose (2‐FF) (10-50 μM) on the migration and invasion of HUCCT1‐TRCs and RBE‐TRCs. 2-FF treatment significantly inhibited migration and invasion in ICC-TRCs.
2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Nov 28:e2407519. [Abstract]
Phalloidin staining showed the inhibition of 2F-Peracetyl-Fucose (2‐FF) (50 μM) on F‐actin of HUCCT1‐TRCs and RBE‐TRCs. Red, phalloidin; blue, DAPI. Scale, 20 µm.
2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Nov 28:e2407519. [Abstract]
G‐actin/F‐actin in vivo assay kit and IB was used to investigate the alteration of F‐/G‐actin induced by 2F-Peracetyl-Fucose (2‐FF) (50 μM) treatment (n = 3, t‐test). 2‐FF treatment significantly inhibited F/G‐actin ratio in ICC‐TRCs.
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JCI Insight
Age-related dysregulation of intestinal epithelium fucosylation is linked to an increased risk of colon cancer. [Abstract]2024 Mar 8;9(5):e167676. PMID: 38456503
2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: JCI Insight. 2024 Mar 8;9(5):e167676. [Abstract]
2F-Peracetyl-Fucose (2-FF) (300 μM; 24 h) markedly downregulated the expression levels of FUT2 and FUT8 in DLD-1 cells, indicating that 2-FF effectively suppressed epithelial fucosylation.
2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: JCI Insight. 2024 Mar 8;9(5):e167676. [Abstract]
Typical images (left) and corresponding statistical results (right) of Transwell invasion and migration assays in DLD-1 cells treated with vehicle, RSV, and 2F-Peracetyl-Fucose (2FF). 2F-Peracetyl-Fucose (2FF, 300 μM, 24 h) inhibited the migration of DLD-1 cells. Scale bar = 50 μm.
2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: JCI Insight. 2024 Mar 8;9(5):e167676. [Abstract]
Representative images (left) and quantification (right) of wound healing assay at 0 and 24 hours in DLD-1 cells treated with vehicle, RSV, and 2F-Peracetyl-Fucose (2FF). 2F-Peracetyl-Fucose (2FF, 300 μM, 24 h) inhibited the migration of DLD-1 cells. Light blue line marks scratch wound edges. Scale bar = 200 μm. Scratched areas were quantified as a percentage after 24 hours relative to 0 hours.
2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: JCI Insight. 2024 Mar 8;9(5):e167676. [Abstract]
Measurement of cell proliferation using CCK8 assays. 2F-Peracetyl-Fucose (2FF, 300 μM, 24 h) inhibited the cell proliferation of DLD-1 cells.
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Solvent & Solubility
DMSO : 100 mg/mL (342.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 5 mg/mL (17.11 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.55 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.55 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Martina Zimmermann, et al. Impact of Acetylated and Non-Acetylated Fucose Analogues on IgG Glycosylation. Antibodies (Basel). 2019 Jan 10;8(1):9. [Content Brief]
[2]. Desiderio V, et al. Increased fucosylation has a pivotal role in invasive and metastatic properties of head and neck cancer stem cells. Oncotarget. 2015 Jan 1;6(1):71-84. [Content Brief]
[3]. Park S, et al. Altered expression of fucosylation pathway genes is associated with poor prognosis and tumor metastasis in non‑small cell lung cancer. Int J Oncol. 2020 Feb;56(2):559-567. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O / DMSO | 1 mM | 3.4216 mL | 17.1081 mL | 34.2161 mL | 85.5403 mL |
| 5 mM | 0.6843 mL | 3.4216 mL | 6.8432 mL | 17.1081 mL | |
| 10 mM | 0.3422 mL | 1.7108 mL | 3.4216 mL | 8.5540 mL | |
| 15 mM | 0.2281 mL | 1.1405 mL | 2.2811 mL | 5.7027 mL | |
| DMSO | 20 mM | 0.1711 mL | 0.8554 mL | 1.7108 mL | 4.2770 mL |
| 25 mM | 0.1369 mL | 0.6843 mL | 1.3686 mL | 3.4216 mL | |
| 30 mM | 0.1141 mL | 0.5703 mL | 1.1405 mL | 2.8513 mL | |
| 40 mM | 0.0855 mL | 0.4277 mL | 0.8554 mL | 2.1385 mL | |
| 50 mM | 0.0684 mL | 0.3422 mL | 0.6843 mL | 1.7108 mL | |
| 60 mM | 0.0570 mL | 0.2851 mL | 0.5703 mL | 1.4257 mL | |
| 80 mM | 0.0428 mL | 0.2139 mL | 0.4277 mL | 1.0693 mL | |
| 100 mM | 0.0342 mL | 0.1711 mL | 0.3422 mL | 0.8554 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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