2F-Peracetyl-Fucose (Synonyms: 1,3,4-Tri-O-acetyl-2-deoxy-2-fluoro-L-fucopyranos)

Name: 2F-Peracetyl-Fucose
Brand: MedChemExpress
SKU: HY-W096600
Rating: 5.0 (3 reviews)

Cat. No.: HY-W096600 Purity: 99.54%: Data Sheet
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2F-Peracetyl-Fucose (1,3,4-Tri-O-acetyl-2-deoxy-2-fluoro-L-fucopyranos) acts as a potent fucosyltransferase (FUT) inhibitor.

For research use only. We do not sell to patients.

CAS No. : 188783-78-0

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
250 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE 2F-Peracetyl-Fucose

RT-PCR

Cell Imaging/Staining

Cell Migration/Invasion Assay

Cell Proliferation/Viability Assay

: 2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Nov 28:e2407519. [Abstract]; Inhibition of 2F-Peracetyl-Fucose (2‐FF) (10-50 μM) on colony spheroid growth in HUCCT1‐TRCs and RBE‐TRCs. The representative images of colony spheroids on day 8 (treatment from day3). 2‐FF treatment significantly inhibited the growth of colony spheroids.

: 2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Nov 28:e2407519. [Abstract]; Effects of 2F-Peracetyl-Fucose (2‐FF) (10-50 μM) on the migration and invasion of HUCCT1‐TRCs and RBE‐TRCs. 2-FF treatment significantly inhibited migration and invasion in ICC-TRCs.

: 2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Nov 28:e2407519. [Abstract]; Phalloidin staining showed the inhibition of 2F-Peracetyl-Fucose (2‐FF) (50 μM) on F‐actin of HUCCT1‐TRCs and RBE‐TRCs. Red, phalloidin; blue, DAPI. Scale, 20 µm.

: 2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Nov 28:e2407519. [Abstract]; G‐actin/F‐actin in vivo assay kit and IB was used to investigate the alteration of F‐/G‐actin induced by 2F-Peracetyl-Fucose (2‐FF) (50 μM) treatment (n = 3, t‐test). 2‐FF treatment significantly inhibited F/G‐actin ratio in ICC‐TRCs.

: 2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: JCI Insight. 2024 Mar 8;9(5):e167676.; 2F-Peracetyl-Fucose (2-FF) (300 μM; 24 h) markedly downregulated the expression levels of FUT2 and FUT8 in DLD-1 cells, indicating that 2-FF effectively suppressed epithelial fucosylation.

: 2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: JCI Insight. 2024 Mar 8;9(5):e167676.; Typical images (left) and corresponding statistical results (right) of Transwell invasion and migration assays in DLD-1 cells treated with vehicle, RSV, and 2F-Peracetyl-Fucose (2FF). 2F-Peracetyl-Fucose (2FF, 300 μM, 24 h) inhibited the migration of DLD-1 cells. Scale bar = 50 μm.

: 2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: JCI Insight. 2024 Mar 8;9(5):e167676.; Representative images (left) and quantification (right) of wound healing assay at 0 and 24 hours in DLD-1 cells treated with vehicle, RSV, and 2F-Peracetyl-Fucose (2FF). 2F-Peracetyl-Fucose (2FF, 300 μM, 24 h) inhibited the migration of DLD-1 cells. Light blue line marks scratch wound edges. Scale bar = 200 μm. Scratched areas were quantified as a percentage after 24 hours relative to 0 hours.

: 2F-Peracetyl-Fucose purchased from MedChemExpress. Usage Cited in: JCI Insight. 2024 Mar 8;9(5):e167676.; Measurement of cell proliferation using CCK8 assays. 2F-Peracetyl-Fucose (2FF, 300 μM, 24 h) inhibited the cell proliferation of DLD-1 cells.

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Description

2F-Peracetyl-Fucose (1,3,4-Tri-O-acetyl-2-deoxy-2-fluoro-L-fucopyranos) acts as a potent fucosyltransferase (FUT) inhibitor^[1].

In Vitro

2F-Peracetyl-Fucose (64 μM, 72 hours) inhibits orosphere formation and invasion in UMSCC14B and UMSCC103 cells^[2].
2F-PAF (25-200 μM, 48 hours) inhibits migration ability in NCI-H3122 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Invasion Assay^[2]

Cell Line:	UMSCC14B, UMSCC103 (head and neck squamous cell carcinoma)
Concentration:	64 μM
Incubation Time:	72 hours
Result:	Treated spheres were smaller with reduced invasion.

Cell Migration Assay ^[3]

Cell Line:	NCI-H3122 (NSCLC)
Concentration:	25-200 μM
Incubation Time:	48 hours
Result:	Reduced number of migrated cells.

In Vivo

2F-PAF (20 μg/ml, i.v., single dose) inhibits tumor metastasis in Calu-1 xenograft nude mouse model^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Calu-1 xenograft BALB/c nude mouse model^[3]
Dosage:	20 μg/ml
Administration:	Intravenous injection (i.v.), single dose
Result:	Reduced tumor colonization ability in the lungs.

Molecular Weight

292.26

Formula

C₁₂H₁₇FO₇

CAS No.

188783-78-0

Appearance

Solid

Color

White to off-white

SMILES

C[C@H]1[C@@H](OC(C)=O)[C@@H](OC(C)=O)[C@H](F)C(OC(C)=O)O1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (342.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 5 mg/mL (17.11 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4216 mL	17.1081 mL	34.2161 mL
5 mM	0.6843 mL	3.4216 mL	6.8432 mL
10 mM	0.3422 mL	1.7108 mL	3.4216 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.55 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.55 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.55 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.54%

Select Batch:

Data Sheet (280 KB) SDS (394 KB)

COA (251 KB) HNMR (268 KB) RP-HPLC (248 KB) MS (104 KB)

Handling Instructions (2659 KB)

References

[1]. Martina Zimmermann, et al. Impact of Acetylated and Non-Acetylated Fucose Analogues on IgG Glycosylation. Antibodies (Basel). 2019 Jan 10;8(1):9. [Content Brief]

[2]. Desiderio V, et al. Increased fucosylation has a pivotal role in invasive and metastatic properties of head and neck cancer stem cells. Oncotarget. 2015 Jan 1;6(1):71-84. [Content Brief]

[3]. Park S, et al. Altered expression of fucosylation pathway genes is associated with poor prognosis and tumor metastasis in non‑small cell lung cancer. Int J Oncol. 2020 Feb;56(2):559-567. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.4216 mL	17.1081 mL	34.2161 mL	85.5403 mL
	5 mM	0.6843 mL	3.4216 mL	6.8432 mL	17.1081 mL
	10 mM	0.3422 mL	1.7108 mL	3.4216 mL	8.5540 mL
	15 mM	0.2281 mL	1.1405 mL	2.2811 mL	5.7027 mL
DMSO	20 mM	0.1711 mL	0.8554 mL	1.7108 mL	4.2770 mL
	25 mM	0.1369 mL	0.6843 mL	1.3686 mL	3.4216 mL
	30 mM	0.1141 mL	0.5703 mL	1.1405 mL	2.8513 mL
	40 mM	0.0855 mL	0.4277 mL	0.8554 mL	2.1385 mL
	50 mM	0.0684 mL	0.3422 mL	0.6843 mL	1.7108 mL
	60 mM	0.0570 mL	0.2851 mL	0.5703 mL	1.4257 mL
	80 mM	0.0428 mL	0.2139 mL	0.4277 mL	1.0693 mL
	100 mM	0.0342 mL	0.1711 mL	0.3422 mL	0.8554 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

2F-Peracetyl-Fucose Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2F-Peracetyl-Fucose188783-78-01,3,4-Tri-O-acetyl-2-deoxy-2-fluoro-L-fucopyranosGlycosyltransferasefucosyltransferaseFUT inhibitorAnti-tumorInhibitorinhibitorinhibit

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2F-Peracetyl-Fucose (Synonyms: 1,3,4-Tri-O-acetyl-2-deoxy-2-fluoro-L-fucopyranos)

Customer Review

2F-Peracetyl-Fucose Related Antibodies

3 Publications Citing Use of MCE 2F-Peracetyl-Fucose

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

2F-Peracetyl-Fucose Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

2F-Peracetyl-Fucose Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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