Art. -Nr.: HY-12743

K-7174

15 Cited Publications

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Based on 15 publication(s) in Google Scholar

K-7174 is an orally active proteasome and GATA inhibitor. K-7174 is a cell adhesion inhibitor. K-7174 induces cell apoptosis. K-7174 shows antitumor activities, it can be used for the research of cancer.

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Reinheit: 99.43%
CAS. Nr.: 191089-59-5
Formel: C₃₃H₄₈N₂O₆
Molecular Weight:568.74

Speicherung:

Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

K-7174 Chemical Structure

CAS. Nr.: 191089-59-5

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Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
Chemical Information
Publications (15)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Bioz

Biologische Aktivität

Beschreibung

In Vitro

K-7174 (10 μM; 1 h) inhibits the adhesion by VCAM-1 and its ligand^[1].
K-7174 (1-30 μM; 1 h) dose-dependently suppresses the VCAM-1 expression with an IC₅₀ value of 14 μM^[1].
K-7174 (1-30 μM; 1 h) dose-dependently suppresses the induction of VCAM-1 mRNA by TNFα with an IC₅₀ value of 9 μM^[1].
K-7174 (10-20 μM; 24 h) dose-dependently rescues Epo production by Hep3B cells^[2].
K-7174 (2.5-30 μM; 24 h) inhibits the binding activity of GATA^[2].
K-7174 (0-25 μM; 72 h) inhibits MM cells growth and induces cell apoptosis^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:

KMS12-BM, U266, and RPMI8226 cell lines
Concentration:

0-25 μM
Incubation Time:

72 h
Result:

Inhibited MM cells growth.

Apoptosis Analysis^[3]

Cell Line:

KMS12-BM, U266, and RPMI8226 cell lines
Concentration:

10 μM
Incubation Time:

48 h
Result:

Significantly increased apoptosis of MM cells with the increasing percentage of annexin-V-positive cells.

In Vivo

K-7174 (30 mg/kg; i.p. once daily for 9 days) reverses the decreasing of hemoglobin concentrations and reticulocyte counts by IL-1β or TNF-α^[2].
K-7174 (75 mg/kg; i.p. once daily for 14 days) inhibits the tumor growth in vivo^[3].
K-7174 (50 mg/kg; p.o. once daily for 14 days) inhibits the tumor growth in vivo and shows a better effect than intraperitoneal injection^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

ICR mice with IL-β or TNF-α injection^[2]
Dosage:

30 mg/kg
Administration:

Intraperitoneal injection; 30 mg/kg once daily for 9 days
Result:

Increased erythropoietin (Epo) production, reticulocyte counts, and hemoglobin (Hb) concentrations

Animal Model:

NOD/SCID mice with murine xenograft^[3]
Dosage:

75 mg/kg
Administration:

Intraperitoneal injection; once daily for 14 days
Result:

Significantly decreased tumor volume, but showed a significant body weight reduction after 10 days.

Animal Model:

NOD/SCID mice with murine xenograft^[3]
Dosage:

50 mg/kg
Administration:

Oral gavage; once daily for 14 days
Result:

Showed an anti-myeloma activity. Proved oral administration is more effective than intraperitoneal injection.

Chemical Information

CAS. Nr. 191089-59-5
Appearance Oil
Molecular Weight 568.74
Formel C₃₃H₄₈N₂O₆
Color Colorless to light yellow
SMILES

COC1=C(OC)C(OC)=CC(/C=C/CCCN2CCN(CCC/C=C/C3=CC(OC)=C(OC)C(OC)=C3)CCC2)=C1
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (15)

All IFWB

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Journal Impact Factor

Adv Sci (Weinh)

Blockade of ZMIZ1-GATA4 Axis Regulation Restores Youthfulness to Aged Cartilage. [Abstract]

2025 Apr;12(16):e2404311. PMID: 40040621
J Clin Invest

PRMT5-mediated FUBP1 methylation accelerates prostate cancer progression. [Abstract]

2024 Aug 15:e175023. PMID: 39146021
Biomaterials

Self-assembled hyaluronic acid nanoparticles for osteoarthritis treatment. [Abstract]

2021 Aug:275:120967. PMID: 34153786
Sci Adv

Androgen receptor-regulated lncRNA PRCAT71 promotes AR signaling through the interaction with KHSRP in prostate cancer. [Abstract]

2025 Apr 11;11(15):eadk6989. PMID: 40203114
Cell Rep Med

Inhibiting 3βHSD1 to eliminate the oncogenic effects of progesterone in prostate cancer. [Abstract]

2022 Mar 15;3(3):100561. PMID: 35492874
Adv Healthc Mater

Unveiling the Impact of Hemodynamics on Endothelial Inflammation-Mediated Hepatocellular Carcinoma Metastasis Using a Biomimetic Vascular Flow Model. [Abstract]

2024 Jul;13(18):e2304439. PMID: 38486060
FASEB J

Differentiation of c-Kit+ CD24+ natural killer cells into myeloid cells in a GATA-2-dependent manner. [Abstract]

2020 Mar;34(3):4462-4481. PMID: 31989715
J Assist Reprod Genet

SIRT5 suppresses the trophoblast cell proliferation, invasion, and migration to promote preeclampsia via desuccinylating HOXB3. [Abstract]

2024 Oct;41(10):2759-2770. PMID: 39145876
Brain Res

Small molecule compound K-7174 attenuates neuropsychiatric manifestations in lupus-prone mice. [Abstract]

2023 Feb 15:1801:148203. PMID: 36521514

K-7174 purchased from MedChemExpress. Usage Cited in: Brain Res. 2023 Feb 15:1801:148203. [Abstract]

K-7174 (30 mg/kg; i.p.; 3 times a week for 6 weeks) improves lupus-like symptoms in MRL/lpr mice. The deposition of IgG and C3 in the kidneys of mice in the MRL/lpr group is significantly higher than that in the control group, but K-7174 can significantly inhibit this deposition.

K-7174 purchased from MedChemExpress. Usage Cited in: Brain Res. 2023 Feb 15:1801:148203. [Abstract]

K-7174 significantly increases the protein and gene levels of ZO1 and Occludin in NPSLE model mouse (MRL/lpr mice) brain.
FEBS Open Bio

Chemokine-like factor 1 (CKLF1) aggravates neointimal hyperplasia through activating the NF-κB /VCAM-1 pathway. [Abstract]

2020 Sep;10(9):1880-1890. PMID: 32741140
Growth Horm IGF Res

Growth hormone regulates deiodinase type 2 and 3 expression via GATA. [Abstract]

2025 May 21:82:101659. PMID: 40413916
Biochem Genet

Let-7c-5p Targeting CHD7 Hinders Cervical Cancer Migration and Invasion by Regulating Cell Adhesion. [Abstract]

2024 Dec 16. PMID: 39680330
Research Square Preprint

2020 Dec.
Seoul National University

2020 May.
bioRxiv

2019 Sep.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (175.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.7583 mL

8.7914 mL

17.5827 mL

5 mM

0.3517 mL

1.7583 mL

3.5165 mL

10 mM

0.1758 mL

0.8791 mL

1.7583 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.40 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.40 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Umetani M, et al. A novel cell adhesion inhibitor, K-7174, reduces the endothelial VCAM-1 induction by inflammatory cytokines, acting through the regulation of GATA. Biochem Biophys Res Commun. 2000 Jun 7;272(2):370-4. [Content Brief]

[2]. Imagawa S, et al. A GATA-specific inhibitor (K-7174) rescues anemia induced by IL-1beta, TNF-alpha, or L-NMMA. FASEB J. 2003 Sep;17(12):1742-4. [Content Brief]

[3]. Kikuchi J, et al. The novel orally active proteasome inhibitor K-7174 exerts anti-myeloma activity in vitro and in vivo by down-regulating the expression of class I histone deacetylases. J Biol Chem. 2013 Aug 30;288(35):25593-602. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.7583 mL	8.7914 mL	17.5827 mL	43.9568 mL
	5 mM	0.3517 mL	1.7583 mL	3.5165 mL	8.7914 mL
	10 mM	0.1758 mL	0.8791 mL	1.7583 mL	4.3957 mL
	15 mM	0.1172 mL	0.5861 mL	1.1722 mL	2.9305 mL
	20 mM	0.0879 mL	0.4396 mL	0.8791 mL	2.1978 mL
	25 mM	0.0703 mL	0.3517 mL	0.7033 mL	1.7583 mL
	30 mM	0.0586 mL	0.2930 mL	0.5861 mL	1.4652 mL
	40 mM	0.0440 mL	0.2198 mL	0.4396 mL	1.0989 mL
	50 mM	0.0352 mL	0.1758 mL	0.3517 mL	0.8791 mL
	60 mM	0.0293 mL	0.1465 mL	0.2930 mL	0.7326 mL
	80 mM	0.0220 mL	0.1099 mL	0.2198 mL	0.5495 mL
	100 mM	0.0176 mL	0.0879 mL	0.1758 mL	0.4396 mL

Keywords

K-7174

15 Cited Publications

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K-7174

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

Anti-Cancer Compound Library

Ubiquitination Compound Library

Pyroptosis Compound Library

Orally Active Compound Library

Anti-Blood Cancer Compound Library

Protease Inhibitor Library

Cell Death Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) K-7174

Biologische Aktivität

Chemical Information

Publications (15)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

K-7174

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

Anti-Cancer Compound Library

Ubiquitination Compound Library

Pyroptosis Compound Library

Orally Active Compound Library

Anti-Blood Cancer Compound Library

Protease Inhibitor Library

Cell Death Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) K-7174

Biologische Aktivität

Chemical Information

Publications (15)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

K-7174 Related Classifications

Art. -Nr.: HY-12743