K-7174 dihydrochloride
Based on 16 publication(s) in Google Scholar
K-7174 dihydrochloride is an orally active proteasome and GATA inhibitor. K-7174 dihydrochloride is a cell adhesion inhibitor. K-7174 dihydrochloride induces cell apoptosis. K-7174 dihydrochloride shows antitumor activities, it can be used for the research of cancer.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté : 98.72%
- CAS No.: 191089-60-8
- Formule: C33H50Cl2N2O6
- Masse moléculaire:641.67
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Stockage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) K-7174 dihydrochloride
More- J Clin Invest. 2024 Aug 15;134(18):e175023. [Abstract]
- Adv Sci (Weinh). 2025 Apr;12(16):e2404311. [Abstract]
- Cell Rep Med. 2022 Mar 15;3(3):100561. [Abstract]
- Sci Adv. 2025 Apr 11;11(15):eadk6989. [Abstract]
- Biomaterials. 2021 Aug:275:120967. [Abstract]
- Adv Healthc Mater. 2024 Jul;13(18):e2304439. [Abstract]
- Acta Pharmacol Sin. 2026 Jul 13.
- FASEB J. 2020 Mar;34(3):4462-4481. [Abstract]
- Brain Res. 2023 Feb 15:1801:148203. [Abstract]
- J Assist Reprod Genet. 2024 Oct;41(10):2759-2770. [Abstract]
- FEBS Open Bio. 2020 Sep;10(9):1880-1890. [Abstract]
- Growth Horm IGF Res. 2025 Oct:82:101659. [Abstract]
- Biochem Genet. 2024 Dec 16. [Abstract]
- Research Square Preprint. 2020 Dec.
- Seoul National University. 2020 May.
- bioRxiv. 2019 Sep.
Customer Validation & Images
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Activité biologique
Description
In Vitro
K-7174 dihydrochloride (10 μM; 1 h) inhibits the adhesion by VCAM-1 and its ligand[1]. K-7174 dihydrochloride (1-30 μM; 1 h) dose-dependently suppresses the VCAM-1 expression with an IC50 value of 14 μM[1]. K-7174 dihydrochloride (1-30 μM; 1 h) dose-dependently suppresses the induction of VCAM-1 mRNA by TNFα with an IC50 value of 9 μM[1]. K-7174 dihydrochloride (10-20 μM; 24 h) dose-dependently rescues Epo production by Hep3B cells[2]. K-7174 dihydrochloride (2.5-30 μM; 24 h) inhibits the binding activity of GATA[2]. K-7174 dihydrochloride (0-25 μM; 72 h) inhibits MM cells growth and induces cell apoptosis[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:KMS12-BM, U266, and RPMI8226 cell lines
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Concentration:0-25 μM
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Incubation Time:72 h
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Result:Inhibited MM cells growth.
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Cell Line:KMS12-BM, U266, and RPMI8226 cell lines
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Concentration:10 μM
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Incubation Time:48 h
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Result:Significantly increased apoptosis of MM cells with the increasing percentage of annexin-V-positive cells.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:ICR mice with IL-β or TNF-α injection[2]
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Dosage:30 mg/kg
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Administration:Intraperitoneal injection; 30 mg/kg once daily for 9 days
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Result:Increased erythropoietin (Epo) production, reticulocyte counts, and hemoglobin (Hb) concentrations.
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Animal Model:NOD/SCID mice with murine xenograft[3]
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Dosage:75 mg/kg
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Administration:Intraperitoneal injection; once daily for 14 days
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Result:Significantly decreased tumor volume, but showed a significant body weight reduction after 10 days.
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Animal Model:NOD/SCID mice with murine xenograft[3]
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Dosage:50 mg/kg
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Administration:Oral gavage; once daily for 14 days
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Result:Showed an anti-myeloma activity. Porved oral administration is more effective than intraperitoneal injection.
Chemical Information
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CAS No. 191089-60-8
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Appearance Solid
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Masse moléculaire 641.67
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Formule C33H50Cl2N2O6
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Color White to yellow
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SMILES
COC1=C(OC)C(OC)=CC(/C=C/CCCN2CCN(CCC/C=C/C3=CC(OC)=C(OC)C(OC)=C3)CCC2)=C1.[H]Cl.[H]Cl
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (16)
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Journal Impact Factor
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Most Recent
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J Clin Invest
2024 Aug 15;134(18):e175023. PMID: 39146021 -
Adv Sci (Weinh)
2025 Apr;12(16):e2404311. PMID: 40040621 -
Cell Rep Med
2022 Mar 15;3(3):100561. PMID: 35492874 -
Sci Adv
Androgen receptor-regulated lncRNA PRCAT71 promotes AR signaling through the interaction with KHSRP in prostate cancer. [Abstract]2025 Apr 11;11(15):eadk6989. PMID: 40203114 -
Biomaterials
2021 Aug:275:120967. PMID: 34153786 -
Adv Healthc Mater
Unveiling the Impact of Hemodynamics on Endothelial Inflammation-Mediated Hepatocellular Carcinoma Metastasis Using a Biomimetic Vascular Flow Model. [Abstract]2024 Jul;13(18):e2304439. PMID: 38486060 -
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FASEB J
Differentiation of c-Kit+ CD24+ natural killer cells into myeloid cells in a GATA-2-dependent manner. [Abstract]2020 Mar;34(3):4462-4481. PMID: 31989715 -
Brain Res
Small molecule compound K-7174 attenuates neuropsychiatric manifestations in lupus-prone mice. [Abstract]2023 Feb 15:1801:148203. PMID: 36521514
K-7174 dihydrochloride purchased from MedChemExpress. Usage Cited in: Brain Res. 2023 Feb 15:1801:148203. [Abstract]
K-7174 (30 mg/kg; i.p.; 3 times a week for 6 weeks) improves lupus-like symptoms in MRL/lpr mice. The deposition of IgG and C3 in the kidneys of mice in the MRL/lpr group is significantly higher than that in the control group, but K-7174 can significantly inhibit this deposition.
K-7174 dihydrochloride purchased from MedChemExpress. Usage Cited in: Brain Res. 2023 Feb 15:1801:148203. [Abstract]
K-7174 significantly increases the protein and gene levels of ZO1 and Occludin in NPSLE model mouse (MRL/lpr mice) brain.
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J Assist Reprod Genet
SIRT5 suppresses the trophoblast cell proliferation, invasion, and migration to promote preeclampsia via desuccinylating HOXB3. [Abstract]2024 Oct;41(10):2759-2770. PMID: 39145876 -
FEBS Open Bio
Chemokine-like factor 1 (CKLF1) aggravates neointimal hyperplasia through activating the NF-κB /VCAM-1 pathway. [Abstract]2020 Sep;10(9):1880-1890. PMID: 32741140 -
Growth Horm IGF Res
2025 Oct:82:101659. PMID: 40413916 -
Biochem Genet
Let-7c-5p Targeting CHD7 Hinders Cervical Cancer Migration and Invasion by Regulating Cell Adhesion. [Abstract]2024 Dec 16. PMID: 39680330 -
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Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (155.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 50 mg/mL (77.92 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (7.79 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (7.79 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 10 mg/mL (15.58 mM); Clear solution; Need ultrasonic
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Protocole
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Apoptosis
Apoptosis, also called programmed cell death, is generally characterized by distinct morphological characteristics.
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TUNEL staining for apoptotic DNA fragmentation
TUNEL staining detects DNA strand breaks by using terminal deoxynucleotidyl transferase to add labeled nucleotides to exposed 3′-OH DNA termini, generating either microscopic staining in fixed cells or tissue sections, or fluorescence/cytometric signal in cell suspensions. TUNEL positivity reflects DNA fragmentation but should not be interpreted alone as definitive apoptosis, because TUNEL can also label necrotic, autolytic, mechanically damaged, or DNA-repair-associated DNA breaks.
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Annexin V plus membrane-impermeant dye apoptosis staining
Annexin V-based apoptosis assays rely on the detection of phosphatidylserine (PS) externalization from the inner leaflet of the plasma membrane to the outer leaflet, an early biochemical hallmark of apoptosis. Fluorescently labeled Annexin V binds PS in a calcium-dependent manner, enabling identification of early apoptotic cells by flow cytometry or fluorescence microscopy. When combined with a membrane-impermeant DNA-binding dye (e. g. , propidium iodide), this approach allows discrimination between viable (Annexin V−/dye−), early apoptotic (Annexin V+/dye−), and late apoptotic or necrotic (Annexin V+/dye+) cell populations by assessing membrane integrity and PS exposure.
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Apoptosis Solutions
Apoptosis is a regulated, generally non-lytic cell-death pathway that removes unwanted, damaged, infected, or abnormal cells through coordinated morphological changes, caspase activation, DNA fragmentation, and membrane remodeling. The intrinsic apoptosis pathway is controlled mainly by mitochondrial outer membrane permeabilization, BCL-2 family proteins, cytochrome c release, apoptosome formation, caspase-9 activation, and downstream executioner caspase-3/7 activation. The extrinsic apoptosis pathway is initiated by death receptors such as Fas, TNFR, and TRAIL receptors, which recruit adaptor proteins and activate caspase-8 before engaging executioner caspases or mitochondrial amplification through BID cleavage. Apoptosis is linked to many phenotypes, including cancer cell killing, tissue homeostasis, immune regulation, neurodegeneration, infection response, and treatment-induced cytotoxicity; unresolved questions include how apoptosis interacts with necroptosis, pyroptosis, ferroptos
Pureté et documentation
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Fiche technique (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Umetani M, et al. A novel cell adhesion inhibitor, K-7174, reduces the endothelial VCAM-1 induction by inflammatory cytokines, acting through the regulation of GATA. Biochem Biophys Res Commun. 2000 Jun 7;272(2):370-4. [Content Brief]
[2]. Imagawa S, et al. A GATA-specific inhibitor (K-7174) rescues anemia induced by IL-1beta, TNF-alpha, or L-NMMA. FASEB J. 2003 Sep;17(12):1742-4. [Content Brief]
[3]. Kikuchi J, et al. The novel orally active proteasome inhibitor K-7174 exerts anti-myeloma activity in vitro and in vivo by down-regulating the expression of class I histone deacetylases. J Biol Chem. 2013 Aug 30;288(35):25593-602. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.5584 mL | 7.7922 mL | 15.5843 mL | 38.9608 mL |
| 5 mM | 0.3117 mL | 1.5584 mL | 3.1169 mL | 7.7922 mL | |
| 10 mM | 0.1558 mL | 0.7792 mL | 1.5584 mL | 3.8961 mL | |
| 15 mM | 0.1039 mL | 0.5195 mL | 1.0390 mL | 2.5974 mL | |
| 20 mM | 0.0779 mL | 0.3896 mL | 0.7792 mL | 1.9480 mL | |
| 25 mM | 0.0623 mL | 0.3117 mL | 0.6234 mL | 1.5584 mL | |
| 30 mM | 0.0519 mL | 0.2597 mL | 0.5195 mL | 1.2987 mL | |
| 40 mM | 0.0390 mL | 0.1948 mL | 0.3896 mL | 0.9740 mL | |
| 50 mM | 0.0312 mL | 0.1558 mL | 0.3117 mL | 0.7792 mL | |
| 60 mM | 0.0260 mL | 0.1299 mL | 0.2597 mL | 0.6493 mL | |
| DMSO | 80 mM | 0.0195 mL | 0.0974 mL | 0.1948 mL | 0.4870 mL |
| 100 mM | 0.0156 mL | 0.0779 mL | 0.1558 mL | 0.3896 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
K-7174 dihydrochloride Related Classifications
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