Cat. No.: HY-12743A

K-7174 dihydrochloride

15 Cited Publications

Customer Review

Based on 15 publication(s) in Google Scholar

K-7174 dihydrochloride is an orally active proteasome and GATA inhibitor. K-7174 dihydrochloride is a cell adhesion inhibitor. K-7174 dihydrochloride induces cell apoptosis. K-7174 dihydrochloride shows antitumor activities, it can be used for the research of cancer.

For research use only. We do not sell to patients.

Purity: 98.72%
CAS No.: 191089-60-8
Formula: C₃₃H₅₀Cl₂N₂O₆
Molecular Weight:641.67

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

K-7174 dihydrochloride Chemical Structure

CAS No.: 191089-60-8

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (15)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

In Vitro

K-7174 dihydrochloride (10 μM; 1 h) inhibits the adhesion by VCAM-1 and its ligand^[1].
K-7174 dihydrochloride (1-30 μM; 1 h) dose-dependently suppresses the VCAM-1 expression with an IC₅₀ value of 14 μM^[1].
K-7174 dihydrochloride (1-30 μM; 1 h) dose-dependently suppresses the induction of VCAM-1 mRNA by TNFα with an IC₅₀ value of 9 μM^[1].
K-7174 dihydrochloride (10-20 μM; 24 h) dose-dependently rescues Epo production by Hep3B cells^[2].
K-7174 dihydrochloride (2.5-30 μM; 24 h) inhibits the binding activity of GATA^[2].
K-7174 dihydrochloride (0-25 μM; 72 h) inhibits MM cells growth and induces cell apoptosis^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:

KMS12-BM, U266, and RPMI8226 cell lines
Concentration:

0-25 μM
Incubation Time:

72 h
Result:

Inhibited MM cells growth.

Apoptosis Analysis^[3]

Cell Line:

KMS12-BM, U266, and RPMI8226 cell lines
Concentration:

10 μM
Incubation Time:

48 h
Result:

Significantly increased apoptosis of MM cells with the increasing percentage of annexin-V-positive cells.

In Vivo

K-7174 dihydrochloride (30 mg/kg; i.p. once daily for 9 days) reverses the decreasing of hemoglobin concentrations and reticulocyte counts by IL-1β or TNF-α^[2].
K-7174 dihydrochloride (75 mg/kg; i.p. once daily for 14 days) inhibits the tumor growth in vivo^[3].
K-7174 dihydrochloride (50 mg/kg; p.o. once daily for 14 days) inhibits the tumor growth in vivo and shows a better effect than intraperitoneal injection^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

ICR mice with IL-β or TNF-α injection^[2]
Dosage:

30 mg/kg
Administration:

Intraperitoneal injection; 30 mg/kg once daily for 9 days
Result:

Increased erythropoietin (Epo) production, reticulocyte counts, and hemoglobin (Hb) concentrations.

Animal Model:

NOD/SCID mice with murine xenograft^[3]
Dosage:

75 mg/kg
Administration:

Intraperitoneal injection; once daily for 14 days
Result:

Significantly decreased tumor volume, but showed a significant body weight reduction after 10 days.

Animal Model:

NOD/SCID mice with murine xenograft^[3]
Dosage:

50 mg/kg
Administration:

Oral gavage; once daily for 14 days
Result:

Showed an anti-myeloma activity. Porved oral administration is more effective than intraperitoneal injection.

Chemical Information

CAS No. 191089-60-8
Appearance Solid
Molecular Weight 641.67
Formula C₃₃H₅₀Cl₂N₂O₆
Color White to yellow
SMILES

COC1=C(OC)C(OC)=CC(/C=C/CCCN2CCN(CCC/C=C/C3=CC(OC)=C(OC)C(OC)=C3)CCC2)=C1.[H]Cl.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (15)

All IFWB

Sort by:

Journal Impact Factor

J Clin Invest

PRMT5-mediated FUBP1 methylation accelerates prostate cancer progression. [Abstract]

2024 Aug 15;134(18):e175023. PMID: 39146021
Adv Sci (Weinh)

Blockade of ZMIZ1-GATA4 Axis Regulation Restores Youthfulness to Aged Cartilage. [Abstract]

2025 Apr;12(16):e2404311. PMID: 40040621
Cell Rep Med

Inhibiting 3βHSD1 to eliminate the oncogenic effects of progesterone in prostate cancer. [Abstract]

2022 Mar 15;3(3):100561. PMID: 35492874
Sci Adv

Androgen receptor-regulated lncRNA PRCAT71 promotes AR signaling through the interaction with KHSRP in prostate cancer. [Abstract]

2025 Apr 11;11(15):eadk6989. PMID: 40203114
Biomaterials

Self-assembled hyaluronic acid nanoparticles for osteoarthritis treatment. [Abstract]

2021 Aug:275:120967. PMID: 34153786
Adv Healthc Mater

Unveiling the Impact of Hemodynamics on Endothelial Inflammation-Mediated Hepatocellular Carcinoma Metastasis Using a Biomimetic Vascular Flow Model. [Abstract]

2024 Jul;13(18):e2304439. PMID: 38486060
FASEB J

Differentiation of c-Kit+ CD24+ natural killer cells into myeloid cells in a GATA-2-dependent manner. [Abstract]

2020 Mar;34(3):4462-4481. PMID: 31989715
Brain Res

Small molecule compound K-7174 attenuates neuropsychiatric manifestations in lupus-prone mice. [Abstract]

2023 Feb 15:1801:148203. PMID: 36521514

K-7174 dihydrochloride purchased from MedChemExpress. Usage Cited in: Brain Res. 2023 Feb 15:1801:148203. [Abstract]

K-7174 (30 mg/kg; i.p.; 3 times a week for 6 weeks) improves lupus-like symptoms in MRL/lpr mice. The deposition of IgG and C3 in the kidneys of mice in the MRL/lpr group is significantly higher than that in the control group, but K-7174 can significantly inhibit this deposition.

K-7174 dihydrochloride purchased from MedChemExpress. Usage Cited in: Brain Res. 2023 Feb 15:1801:148203. [Abstract]

K-7174 significantly increases the protein and gene levels of ZO1 and Occludin in NPSLE model mouse (MRL/lpr mice) brain.
J Assist Reprod Genet

SIRT5 suppresses the trophoblast cell proliferation, invasion, and migration to promote preeclampsia via desuccinylating HOXB3. [Abstract]

2024 Oct;41(10):2759-2770. PMID: 39145876
FEBS Open Bio

Chemokine-like factor 1 (CKLF1) aggravates neointimal hyperplasia through activating the NF-κB /VCAM-1 pathway. [Abstract]

2020 Sep;10(9):1880-1890. PMID: 32741140
Growth Horm IGF Res

Growth hormone regulates deiodinase type 2 and 3 expression via GATA. [Abstract]

2025 Oct:82:101659. PMID: 40413916
Biochem Genet

Let-7c-5p Targeting CHD7 Hinders Cervical Cancer Migration and Invasion by Regulating Cell Adhesion. [Abstract]

2024 Dec 16. PMID: 39680330
Research Square Preprint

2020 Dec.
Seoul National University

2020 May.
bioRxiv

2019 Sep.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (155.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (77.92 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.5584 mL

7.7922 mL

15.5843 mL

5 mM

0.3117 mL

1.5584 mL

3.1169 mL

10 mM

0.1558 mL

0.7792 mL

1.5584 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 5 mg/mL (7.79 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 5 mg/mL (7.79 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 5 mg/mL (7.79 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 10 mg/mL (15.58 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Umetani M, et al. A novel cell adhesion inhibitor, K-7174, reduces the endothelial VCAM-1 induction by inflammatory cytokines, acting through the regulation of GATA. Biochem Biophys Res Commun. 2000 Jun 7;272(2):370-4. [Content Brief]

[2]. Imagawa S, et al. A GATA-specific inhibitor (K-7174) rescues anemia induced by IL-1beta, TNF-alpha, or L-NMMA. FASEB J. 2003 Sep;17(12):1742-4. [Content Brief]

[3]. Kikuchi J, et al. The novel orally active proteasome inhibitor K-7174 exerts anti-myeloma activity in vitro and in vivo by down-regulating the expression of class I histone deacetylases. J Biol Chem. 2013 Aug 30;288(35):25593-602. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	1.5584 mL	7.7922 mL	15.5843 mL	38.9608 mL
	5 mM	0.3117 mL	1.5584 mL	3.1169 mL	7.7922 mL
	10 mM	0.1558 mL	0.7792 mL	1.5584 mL	3.8961 mL
	15 mM	0.1039 mL	0.5195 mL	1.0390 mL	2.5974 mL
	20 mM	0.0779 mL	0.3896 mL	0.7792 mL	1.9480 mL
	25 mM	0.0623 mL	0.3117 mL	0.6234 mL	1.5584 mL
	30 mM	0.0519 mL	0.2597 mL	0.5195 mL	1.2987 mL
	40 mM	0.0390 mL	0.1948 mL	0.3896 mL	0.9740 mL
	50 mM	0.0312 mL	0.1558 mL	0.3117 mL	0.7792 mL
	60 mM	0.0260 mL	0.1299 mL	0.2597 mL	0.6493 mL
DMSO	80 mM	0.0195 mL	0.0974 mL	0.1948 mL	0.4870 mL
DMSO	100 mM	0.0156 mL	0.0779 mL	0.1558 mL	0.3896 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

K-7174 dihydrochloride

15 Cited Publications

100 mg Get Quote

Subscribe to the

MedChemExpress

Newsletter

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

K-7174 dihydrochloride

Bioactive Compound Library Max

Bioactive Compound Library

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

Anti-Cancer Compound Library

Ubiquitination Compound Library

Pyroptosis Compound Library

Orally Active Compound Library

Anti-Blood Cancer Compound Library

Protease Inhibitor Library

Cell Death Library

Anti-Hematopathy Compound Library

MCE Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) K-7174 dihydrochloride

Biological Activity

Chemical Information

Publications (15)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

K-7174 dihydrochloride

Bioactive Compound Library Max

Bioactive Compound Library

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

Anti-Cancer Compound Library

Ubiquitination Compound Library

Pyroptosis Compound Library

Orally Active Compound Library

Anti-Blood Cancer Compound Library

Protease Inhibitor Library

Cell Death Library

Anti-Hematopathy Compound Library

MCE Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) K-7174 dihydrochloride

Biological Activity

Chemical Information

Publications (15)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

K-7174 dihydrochloride Related Classifications

HY-12743A Related Classifications