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E.coli
Expression System -
E.coli Cell-Free Expression System
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Yeast
Expression System -
Mammalian Expression System
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Insect
Expression System
E.coli Expression System
MedChemExpress (MCE) offers a one-stop, customized E.coli protein expression service, including codon optimization, gene synthesis and subcloning, small-scale expression testing, amplified expression and purification, quality control, and delivery. We have an established protein refolding platform to recover and refold inclusion bodies into soluble proteins with biological activity. The overall success rate of our E.coli expression system exceeds 98%. Customers can flexibly choose options that meet their needs, experiencing unparalleled high-quality and competitively priced E.coli protein expression services.
Advantages of the E.coli expression system
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Has a transparent genetic background and a relatively simple structure.
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Short turnaround time and high cell density.
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High protein expression level and easy to amplify.
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A rich selection of expression vectors and fusion tags.
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Easy manipulation to achieve optimal expression.
Service Process
- Codon optimization; gene synthesis.
- Subcloning into appropriate expression vector.
- Transform plasmids into DH5α or TOP10 competent cells.
- Plasmid sequencing and preparation.
1-2 weeks
- Transform plasmids into host cells, e.g.BL21(DE3), BL21(DE3)pLysS, Rossetta(DE3), SHuffle T7, etc..
- Select a single colony for small-scale induced expression.
- Determine optimal expression conditions.
- Purified protein
- QC COA /Project report
1 week
- Scale up protein expression using optimized conditions.
- Multiple-methods purification to obtain sufficient amounts of high-purity soluble proteins.
1-2 weeks
- Purity analysis by SDS-PAGE
- WB/HPLC detection (Optional)
- Bradford assay for quantitation
- Provide COA/report
1 week
Gene Synthesis, Protein Construct Design
- Codon optimization; gene synthesis.
- Subcloning into appropriate expression vector.
- Transform plasmids into DH5α or TOP10 competent cells.
- Plasmid sequencing and preparation.
Deliverable
Purified protein
QC COA /Project report
Lead Time
1-2 weeks
Protein Expression Evaluation, Optimization
- Transform plasmids into host cells, e.g. BL21(DE3), BL21(DE3)pLysS, Rossetta(DE3), SHuffle T7, etc.
- Select a single colony for small-scale induced expression.
- Determine optimal expression conditions.
Deliverable
Purified protein
QC COA /Project report
Lead Time
1-2 weeks
Amplified expression and purification
- Scale up protein expression using optimized conditions.
- Multiple methods purification to obtain sufficient amounts of high-purity soluble proteins.
Deliverable
Purified protein
QC COA /Project report
Lead Time
1-2 weeks
QC & Delivery
- Purity analysis by SDS-PAGE
- WB/HPLC detection (Optional)
- Bradford assay for quantitation
- Provide COA/report
Deliverable
Purified protein
QC COA /Project report
Lead Time
1-2 weeks
E.coli Cell-Free Expression System
E.coli Cell-Free Protein Synthesis System (CFPS) is a new protein preparation system that is different from traditional expression systems. It is a transcription/translation system based on extracts. By adding exogenous target mRNA or DNA templates, utilizing ribosomes, RNA polymerase, ATP and other substances in the cell extracts, and continuously adding some raw materials needed for protein synthesis such as amino acids, nucleotides, salt ions, etc., it synthesizes proteins in an extracellular environment without relying on living cells.
E.coli Cell-Free Expression System
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No need for cell culture or transfection; efficient and convenient.
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Reaction conditions are controllable, open and flexible.
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Reaction system is easy to scale up, enabling large-scale preparation.
Service Process
- Codon optimization; gene synthesis.
- Subcloning into pET series, pCold series and pBV220 vectors.
- Plasmid sequencing and preparation.
1-2 weeks
- Simultaneously detect the protein expression under different vectors and reaction conditions.
- Determine optimal expression conditions.
- Purified protein
- QC COA /Project report
1 week
- Scale up protein expression using optimized conditions.
- Multiple-methods purification to obtain sufficient amounts of high-purity soluble proteins.
1-2 weeks
- Purity analysis by SDS-PAGE
- WB/HPLC detection (Optional)
- Bradford assay for quantitation
- Provide COA/report
1 week
Gene Synthesis, Protein Construct Design
- Codon optimization; gene synthesis.
- Subcloning into appropriate expression vector.
- Plasmid sequencing and preparation.
Deliverable
Purified protein
QC COA /Project report
Lead Time
1-2 weeks
Protein Expression Evaluation, Optimization
- Simultaneously detect the protein expression under different vectors and reaction conditions.
- Determine optimal expression conditions.
Deliverable
Purified protein
QC COA /Project report
Lead Time
1 week
Amplified expression and purification
- Scale up protein expression using optimized conditions.
- Multiple methods purification to obtain sufficient amounts of high-purity soluble proteins.
Deliverable
Purified protein
QC COA /Project report
Lead Time
1-2 weeks
QC & Delivery
- Purity analysis by SDS-PAGE
- WB/HPLC detection (Optional)
- Bradford assay for quantitation
- Provide COA/report
Deliverable
Purified protein
QC COA /Project report
Lead Time
1 week
Yeast Expression Syste
The yeast expression system mainly uses Saccharomyces cerevisiae and Pichia pastoris. Pichia pastoris is a rapidly developing and widely used eukaryotic expression system. It has two main advantages. On one hand, protein post-translational modifications can occur in yeast, which has a lower cost than mammalian expression systems. On the other hand, the heterologous proteins it expresses are first produced inside yeast cells and then secreted into the extracellular matrix through the yeast's secretion system, resulting in high-purity proteins that have undergone folding and modifications.
Yeast Expression Syste
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Has the post-translational modification function of eukaryotic cells.
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Fast growth rate and capable of high-density culture.
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High level of protein expression, conducive to purification.
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Can strictly regulate the expression of exogenous genes.
Service Process
- Codon optimization; gene synthesis.
- Subcloning into an appropriate expression vector, e.g.: pPIC9K, pPIC3.5K, etc..
- Plasmid sequencing and preparation.
1-2 weeks
- Linearize the plasmid and transform plasmids into host cells, e.g.: GS115, SMD1168, KM71, etc.
- Determine optimal expression conditions.
- Purified protein
- QC COA /Project report
2-3 weeks
- Scale up protein expression using optimized conditions.
- Multiple-methods purification to obtain sufficient amounts of high-purity soluble proteins.
2-3 weeks
- Purity analysis by SDS-PAGE
- WB/HPLC detection (Optional)
- Bradford assay for quantitation
- Provide COA/report
1 week
Gene Synthesis, Protein Construct Design
- Codon optimization; gene synthesis.
- Subcloning into an appropriate expression vector, e.g.: pPIC9K, pPIC3.5K, etc..
- Transform plasmids into DH5α or TOP10 competent cells.
- Plasmid sequencing and preparation.
Deliverable
Purified protein
QC COA /Project report
Lead Time
1-2 weeks
Protein Expression Evaluation, Optimization
- Linearize the plasmid and transform plasmids into host cells, e.g.: GS115, SMD1168, KM71, etc.
- screening positive colonies for small-scale induced expression.
- Determine optimal expression conditions.
Deliverable
Purified protein
QC COA /Project report
Lead Time
2-3 weeks
Amplified expression and purification
- Scale up protein expression using optimized conditions.
- Multiple methods purification to obtain sufficient amounts of high-purity soluble proteins.
Deliverable
Purified protein
QC COA /Project report
Lead Time
1-2 weeks
QC & Delivery
- Purity analysis by SDS-PAGE
- WB/HPLC detection (Optional)
- Bradford assay for quantitation
- Provide COA/report
Deliverable
Purified protein
QC COA /Project report
Lead Time
1 week
Mammalian Expression System
Mammalian cell expression systems can achieve correct protein folding and complex post-translational modifications, making the proteins expressed closer to natural proteins than those from other eukaryotic systems. This has been widely used in vaccine, antibody, and protein development. However, the yield of its transient expression system is relatively low, often requiring scale-up to obtain sufficient proteins, leading to increased cycle times and economic costs. We have a wealth of different series expression and proprietary vectors, which can stably and efficiently express sufficient quantities of recombinant proteins and antibodies.
Mammalian Expression System
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Can achieve complex post-translational modifications such as glycosylation, resulting in higher biological activity.
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High protein expression level, with solid control elements controlling protein expression.
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Transient transfection allows rapid acquisition of a certain amount of secreted soluble protein.
Service Process
- Codon optimization; gene synthesis.
- Subcloning into pTT3, pCEP4, pCMV and pCDNA series expression vectors.
- Transform plasmids into DH5α or TOP10 competent cells.
- Plasmid sequencing and preparation.
1-2 weeks
- Transient transfection of HEK293F, HEK293T, CHO and other cells.
- Determine optimal expression conditions.
- Purified protein
- QC COA /Project report
1-2 weeks
- Scale up protein expression using optimized conditions.
- Multiple-methods purification to obtain sufficient amounts of high-purity soluble proteins.
1-2 weeks
- Purity analysis by SDS-PAGE
- WB/HPLC detection (Optional)
- Bradford assay for quantitation
- Provide COA/report
1 week
Gene Synthesis, Protein Construct Design
- Codon optimization; gene synthesis.
- Subcloning into pTT3, pCEP4, pCMV and pCDNA series expression vectors.
- Transform plasmids into DH5α or TOP10 competent cells.
- Plasmid sequencing and preparation.
Deliverable
Purified protein
QC COA /Project report
Lead Time
1-2 weeks
Protein Expression Evaluation, Optimization
- Transient transfection of HEK293F, HEK293T, CHO and other cells.
- Determine optimal expression conditions.
Deliverable
Purified protein
QC COA /Project report
Lead Time
1-2 weeks
Amplified expression and purification
- Scale up protein expression using optimized conditions.
- Multiple methods purification to obtain sufficient amounts of high-purity soluble proteins.
Deliverable
Purified protein
QC COA /Project report
Lead Time
1-2 weeks
QC & Delivery
- Purity analysis by SDS-PAGE
- WB/HPLC detection (Optional)
- Bradford assay for quantitation
- Provide COA/report
Deliverable
Purified protein
QC COA /Project report
Lead Time
1 week
Insect Expression System
The Baculovirus-Insect Cell Expression System is one of the eukaryotic expression systems. This system uses baculovirus-infected insect cells for the expression, purification, and large-scale production of recombinant proteins. Due to the large baculovirus genome and multiple restriction enzyme cut sites on its genome, it has strong plasticity in accommodating exogenous genes and advantages in expressing sizeable molecular weight proteins. By modifying the baculovirus to become a highly efficient recombinant viral vector for expressing target proteins, MedChemExpress (MCE) can provide you with expression development for Sf9 and Hi-5 insect cell lines.
Insect Expression System
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High safety: the baculovirus expression vector has high species specificity.
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Large gene capacity, capable of expressing large molecular weight proteins.
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Strong operability and high level of exogenous gene expression.
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Has similar post-translational modifications, such as glycosylation to mammalian cells, resulting in high biological activity.
Service Process
- Codon optimization; gene synthesis.
- Subcloning into an appropriate expression vector, e.g.:pFastBac1, pFastBacHT, etc..
- Transform plasmids into DH5α or TOP10 competent cells.
- Plasmid sequencing and preparation.
1-2 weeks
- Transform DH10Bac cells to get recombinant Bacmid.
- Transfection of insect cells with recombinant Bacmid DNA.
- Based on protein demand and preliminary expression results, Prepare P1 generation virus (low titer) and P2 generation virus (high titer).
- Purified protein
- QC COA /Project report
2-3 weeks
- Infect insect cells with P1 or P2 generation viruses using optimized conditions.
- Multiple-methods purification to obtain sufficient amounts of high-purity soluble proteins.
1-2 weeks
- Purity analysis by SDS-PAGE
- WB/HPLC detection (Optional)
- Bradford assay for quantitation
- Provide COA/report
1 week
Gene Synthesis, Protein Construct Design
- Codon optimization; gene synthesis.
- Subcloning into pTT3, pCEP4, pCMV and pCDNA series expression vectors.
- Transform plasmids into DH5α or TOP10 competent cells.
- Plasmid sequencing and preparation.
Deliverable
Purified protein
QC COA /Project report
Lead Time
1-2 weeks
Protein Expression Evaluation, Optimization
- Transform DH10Bac cells to get recombinant Bacmid.
- Transfection of insect cells with recombinant Bacmid DNA.
- Based on protein demand and preliminary expression results, Prepare P1 generation virus (low titer) and P2 generation virus (high titer).
Deliverable
Purified protein
QC COA /Project report
Lead Time
2-3 weeks
Amplified expression and purification
- Infect insect cells with P1 or P2 generation viruses using optimized conditions.
- Multiple methods purification to obtain sufficient amounts of high-purity soluble proteins.
Deliverable
Purified protein
QC COA /Project report
Lead Time
1-2 weeks
QC & Delivery
- Purity analysis by SDS-PAGE
- WB/HPLC detection (Optional)
- Bradford assay for quantitation
- Provide COA/report
Deliverable
Purified protein
QC COA /Project report
Lead Time
1 week
Expression Services
Bulk and Custom Product Services
Each project of recombinant protein customization requires evaluation to determine the corresponding plan and price. For further information on service prices or technical details, please send an email to [email protected] Or call 609-228-6898 to contact us directly.