Nodinitib-1
Based on 12 publication(s) in Google Scholar
Nodinitib-1 (ML130;CID-1088438) is a NOD1 inhibitor with an IC50 of 0.56 μM.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.94%
- CAS 番号: 799264-47-4
- 分子式: C14H13N3O2S
- 分子量:287.34
-
保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
-
生物活性
-
化学情報
-
溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
-
Immunology/Inflammation Compound Library
HY-L007
-
NF-κB Signaling Compound Library
HY-L014
-
Antioxidant Compound Library
HY-L037
-
Oxygen Sensing Compound Library
HY-L045
-
Pyroptosis Compound Library
HY-L059
-
Targeted Diversity Library
HY-L099
-
Membrane Receptor-targeted Compound Library
HY-L150
-
Autoimmune Disease Compound Library
HY-L156
-
Radioprotector Library
HY-L178
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Bioactive Compound Library
HY-L001
-
High-Throughput Bioactive Compound Library
HY-L205
-
Pattern Recognition Receptors Library
HY-L237
MedChemExpress（MCE）の使用を引用している文献 Nodinitib-1
More- Protein Cell. 2024 May 28;15(6):419-440. [Abstract]
- J Exp Clin Cancer Res. 2023 Sep 9;42(1):236. [Abstract]
- Sci Adv. 2024 Oct 4;10(40):eadp8266. [Abstract]
- Front Immunol. 2018 Jul 2:9:1528. [Abstract]
- Cell Prolif. 2026 Jan 26:e70177. [Abstract]
- Respir Res. 2026 Jan 30;27(1):97. [Abstract]
- Front Pharmacol. 2022 Jul 22;13:920928. [Abstract]
- Int Immunopharmacol. 2024 May 14:134:112254. [Abstract]
- Cell Signal. 2022 May:93:110283. [Abstract]
- Mol Med Rep. 2024 Mar;29(3):36. [Abstract]
- Mol Oral Microbiol. 2021 Jun;36(3):172-181. [Abstract]
- J Biochem Mol Toxicol. 2025 Jan;39(1):e70126. [Abstract]
生物活性
|
NOD1 |
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| HEK-293T | IC50 |
>20 μM
Compound: 13
|
Inhibition of NOD2 (unknown origin) expressed in HEK293T cells coexpressing NF-kappaB driven luciferase reporter gene by HTS primary assay
Inhibition of NOD2 (unknown origin) expressed in HEK293T cells coexpressing NF-kappaB driven luciferase reporter gene by HTS primary assay
|
[PMID: 24707857] |
| HEK-293T | IC50 |
>20 μM
Compound: 1c, ML130
|
Inhibition of NOD-2 mediated NFkappaB activation in HEK293T cells assessed as inhibition of MDP-induced luciferase activity after 14 hrs by reporter gene assay
Inhibition of NOD-2 mediated NFkappaB activation in HEK293T cells assessed as inhibition of MDP-induced luciferase activity after 14 hrs by reporter gene assay
|
[PMID: 22003428] |
| HEK-293T | IC50 |
>20 μM
Compound: 1c, ML130
|
Inhibition of TNFalpha-induced NFkappaB activation in HEK293T cells after 14 hrs by luciferase reporter gene assay
Inhibition of TNFalpha-induced NFkappaB activation in HEK293T cells after 14 hrs by luciferase reporter gene assay
|
[PMID: 22003428] |
| HEK-293T | IC50 |
0.56 μM
Compound: 13
|
Inhibition of NOD1 (unknown origin) expressed in HEK293T cells coexpressing NF-kappaB driven luciferase reporter gene by HTS primary assay
Inhibition of NOD1 (unknown origin) expressed in HEK293T cells coexpressing NF-kappaB driven luciferase reporter gene by HTS primary assay
|
[PMID: 24707857] |
| HEK-293T | IC50 |
0.56 μM
Compound: 1c, ML130
|
Inhibition of NOD-1 mediated NFkappaB activation in HEK293T cells assessed as inhibition of gamma-tri-DAP-induced luciferase activity after 14 hrs by reporter gene assay
Inhibition of NOD-1 mediated NFkappaB activation in HEK293T cells assessed as inhibition of gamma-tri-DAP-induced luciferase activity after 14 hrs by reporter gene assay
|
[PMID: 22003428] |
Nodinitib-1 selectively inhibits IL-8 production induced by NOD1 ligand. Nodinitib-1 also inhibits γ-tri-DAP-induced expression of the prototypical NF-κB target gene IκBα at the mRNA level. Nodinitib-1 inhibits γ-tri-DAP-dependent activation of NF-κB (IκBα phosphorylation and degradation) and MAPK (p38 phosphorylation) signalings, without affecting Akt survival pathway. Nodinitib-1 selectively inhibits responses of primary dendritic cells to NOD1 ligand. Nodinitib-1 reduces cell surface expression of co-stimulatory molecules CD83, CD86 and HLA-DR and also inhibits expression of IL-1β, IL-6 and TNFα elicited by γ-tri-DAP (but not by LPS), without causing cytoxicity[1]. Nodinitib-1 is identified as NOD1-selective molecules from an HTS campaign involving ~290,000 compounds. Nodinitib-1 inhibits γ-tri-DAP-stimulated luciferase production in HEK 293T cells, which has endogenous NOD1 levels at submicromolar concentration as determined in a NF-κB-linked reporter assay[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
-
CAS 番号 799264-47-4
-
性状 Solid
-
分子量 287.34
-
分子式 C14H13N3O2S
-
Color White to off-white
-
SMILES
NC1=NC2=CC=CC=C2N1S(=O)(C3=CC=C(C)C=C3)=O
-
別名
ML130; CID-1088438
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (12)
-
Journal Impact Factor
-
Most Recent
-
Protein Cell
Microbiota enterotoxigenic Bacteroides fragilis-secreted BFT-1 promotes breast cancer cell stemness and chemoresistance through its functional receptor NOD1. [Abstract]2024 May 28;15(6):419-440. PMID: 38437016 -
J Exp Clin Cancer Res
Fusobacterium nucleatum-triggered neutrophil extracellular traps facilitate colorectal carcinoma progression. [Abstract]2023 Sep 9;42(1):236. PMID: 37684625 -
Sci Adv
Activation of NOD1 on tumor-associated macrophages augments CD8+ T cell-mediated antitumor immunity in hepatocellular carcinoma. [Abstract]2024 Oct 4;10(40):eadp8266. PMID: 39356756 -
Front Immunol
A Single Microorganism Epitope Attenuates the Development of Murine Autoimmune Arthritis: Regulation of Dendritic Cells via the Mannose Receptor. [Abstract]2018 Jul 2:9:1528. PMID: 30013572 -
Cell Prolif
Lactate Accelerates Early Angiogenesis and Bone Regeneration Through Macrophage M1 Polarisation. [Abstract]2026 Jan 26:e70177. PMID: 41587535 -
Respir Res
Peroxiredoxin 1 mediates bleomycin-induced acute lung injury in mice via macrophage NOD1/NF-κB axis. [Abstract]2026 Jan 30;27(1):97. PMID: 41618391 -
Front Pharmacol
Nucleotide-Binding Oligomerization Domain 1/Toll-Like Receptor 4 Co-Engagement Promotes Non-Specific Immune Response Against K562 Cancer Cells. [Abstract]2022 Jul 22;13:920928. PMID: 35935855 -
Int Immunopharmacol
High-glucose conditions attenuate the response of macrophages to Legionella pneumophila infection by inhibiting NOD1 and MAPK signaling. [Abstract]2024 May 14:134:112254. PMID: 38749333 -
Cell Signal
2022 May:93:110283. PMID: 35176453 -
Mol Med Rep
2024 Mar;29(3):36. PMID: 38214327 -
Mol Oral Microbiol
2021 Jun;36(3):172-181. PMID: 33715305 -
J Biochem Mol Toxicol
Cancer-Associated Fibroblast-Secreted Exosomes Regulate Macrophage Polarization in Pancreatic Cancer via the NOD1 Pathway. [Abstract]2025 Jan;39(1):e70126. PMID: 39756063
溶剤 & 溶解度
DMSO : 20 mg/mL (69.60 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
プロトコル
RAW264.7 cells are treated with 5 μM of CID-1088438 or CID-44229067 alone, or also infected with S. typhimurium at multiplicity of infection (MOI) of 20 and 200 bacteria per mammalian cell. Cell viability is analyzed two hours after Salmonella infection by measuring ATP levels. Percentage viability is calculated according to the ATP levels of respective non-infected cells. Values presented are averages of two replicates (+ SEM)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
純度とドキュメンテーション
-
データシート (282 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
-
取扱説明書 (2659 KB)
参考文献
[1]. Correa RG, et al. Discovery and characterization of 2-aminobenzimidazole derivatives as selective NOD1 inhibitors. Chem Biol. 2011 Jul 29;18(7):825-32. [Content Brief]
[2]. Hershberger PM, et al. Synthesis and physicochemical characterization of novel phenotypic probes targeting the nuclear factor-kappa B signaling pathway. Beilstein J Org Chem. 2013 May 8;9:900-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4802 mL | 17.4010 mL | 34.8020 mL | 87.0049 mL |
| 5 mM | 0.6960 mL | 3.4802 mL | 6.9604 mL | 17.4010 mL | |
| 10 mM | 0.3480 mL | 1.7401 mL | 3.4802 mL | 8.7005 mL | |
| 15 mM | 0.2320 mL | 1.1601 mL | 2.3201 mL | 5.8003 mL | |
| 20 mM | 0.1740 mL | 0.8700 mL | 1.7401 mL | 4.3502 mL | |
| 25 mM | 0.1392 mL | 0.6960 mL | 1.3921 mL | 3.4802 mL | |
| 30 mM | 0.1160 mL | 0.5800 mL | 1.1601 mL | 2.9002 mL | |
| 40 mM | 0.0870 mL | 0.4350 mL | 0.8700 mL | 2.1751 mL | |
| 50 mM | 0.0696 mL | 0.3480 mL | 0.6960 mL | 1.7401 mL | |
| 60 mM | 0.0580 mL | 0.2900 mL | 0.5800 mL | 1.4501 mL |
Powered by Bioz