MAT2A inhibitor 3
Based on 1 Customer Validation
MAT2A inhibitor 3 is a methionine adenosyltransferase 2A (MAT2A) inhibitor extracted from patent WO2020123395A1, compound 24, has an IC50 of <200 nM. MAT2A inhibitor 3 can be used for the research of cancers.
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- Purity: 99.27%
- CAS No.: 2439271-82-4
- 화학식: C16H14ClN3O
- 분자량:299.75
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
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Biological Activity
IC50: <200 nM (MAT2A)[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
1277 nM
Compound: AZ-28
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Antiproliferative activity against wild type human HCT116 cells assessed as cell viability at incubated for 8 days by multifunctional microplate reader analysis
Antiproliferative activity against wild type human HCT116 cells assessed as cell viability at incubated for 8 days by multifunctional microplate reader analysis
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[PMID: 38818879] |
| HCT-116 | IC50 |
306.4 nM
Compound: AZ-28
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Antiproliferative activity against MTAP-knock out human HCT-116 cells assessed as cell viability at incubated for 8 days by multifunctional microplate reader analysis
Antiproliferative activity against MTAP-knock out human HCT-116 cells assessed as cell viability at incubated for 8 days by multifunctional microplate reader analysis
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[PMID: 38818879] |
MAT2A inhibitor 3 is a potent methionine adenosyltransferase 2A inhibitor, with an IC50 of <200 nM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2439271-82-4
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Appearance Solid
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분자량 299.75
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화학식 C16H14ClN3O
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Color White to off-white
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SMILES
O=C1N(C2=CC=CC=C2)C3=C(C=CC(Cl)=C3)C(N(C)C)=N1
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
용액&용해도
DMSO : 50 mg/mL (166.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.34 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.34 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3361 mL | 16.6806 mL | 33.3611 mL | 83.4028 mL |
| 5 mM | 0.6672 mL | 3.3361 mL | 6.6722 mL | 16.6806 mL | |
| 10 mM | 0.3336 mL | 1.6681 mL | 3.3361 mL | 8.3403 mL | |
| 15 mM | 0.2224 mL | 1.1120 mL | 2.2241 mL | 5.5602 mL | |
| 20 mM | 0.1668 mL | 0.8340 mL | 1.6681 mL | 4.1701 mL | |
| 25 mM | 0.1334 mL | 0.6672 mL | 1.3344 mL | 3.3361 mL | |
| 30 mM | 0.1112 mL | 0.5560 mL | 1.1120 mL | 2.7801 mL | |
| 40 mM | 0.0834 mL | 0.4170 mL | 0.8340 mL | 2.0851 mL | |
| 50 mM | 0.0667 mL | 0.3336 mL | 0.6672 mL | 1.6681 mL | |
| 60 mM | 0.0556 mL | 0.2780 mL | 0.5560 mL | 1.3900 mL | |
| 80 mM | 0.0417 mL | 0.2085 mL | 0.4170 mL | 1.0425 mL | |
| 100 mM | 0.0334 mL | 0.1668 mL | 0.3336 mL | 0.8340 mL |
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