1. Cell Cycle/DNA Damage
    Apoptosis
  2. DNA/RNA Synthesis
    CRISPR/Cas9
    Apoptosis
  3. SCR7

SCR7 

Cat. No.: HY-12742
Handling Instructions

SCR7 is an unstable form that can be autocyclized into a stable form SCR7 pyrazine. SCR7 pyrazine is a DNA ligase IV inhibitor that blocks nonhomologous end-joining (NHEJ) in a ligase IV-dependent manner. SCR7 pyrazine is also a CRISPR/Cas9 enhancer which increases the efficiency of Cas9-mediated homology-directed repair (HDR). SCR7 pyrazine induces cell apoptosis and has anticancer activity.

For research use only. We do not sell to patients.

SCR7 Chemical Structure

SCR7 Chemical Structure

CAS No. : 1533426-72-0

Size Price Stock Quantity
10 mM * 1  mL in DMSO USD 92 In-stock
Estimated Time of Arrival: December 31
5 mg USD 84 In-stock
Estimated Time of Arrival: December 31
10 mg USD 144 In-stock
Estimated Time of Arrival: December 31
50 mg USD 420 In-stock
Estimated Time of Arrival: December 31
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Based on 4 publication(s) in Google Scholar

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Description

SCR7 is an unstable form that can be autocyclized into a stable form SCR7 pyrazine. SCR7 pyrazine is a DNA ligase IV inhibitor that blocks nonhomologous end-joining (NHEJ) in a ligase IV-dependent manner. SCR7 pyrazine is also a CRISPR/Cas9 enhancer which increases the efficiency of Cas9-mediated homology-directed repair (HDR). SCR7 pyrazine induces cell apoptosis and has anticancer activity[1][2].

IC50 & Target[1]

DNA Ligase IV

 

CRISPR/Cas9

 

In Vitro

SCR7 (SCR7 pyrazine; 20-100 μM; 24 hours; MCF7 cells) treatment interferes with NHEJ in cells, leading to accumulation of unrepaired double-strand breaks (DSBs)[1].
SCR7 (SCR7 pyrazine) treatment shows a dose-dependent decrease in cell proliferation with IC50 values of 40 μM, 34 μM, 44 μM, 8.5 μM, 120 μM, 10 μM and 50 μM for MCF7, A549, HeLa, T47D, A2780, HT1080 and Nalm6 cells, respectively[1].
In MCF7 cells, SCR7 (SCR7 pyrazine; 20, 40 μM) treatment increases phosphorylation of ATM and activates p53, decreases MDM2, BCL2, resulting in activation of proapoptotic proteins, PUMA and BAX. And the shorter fragments of MCL1, PARP1, Caspase 3, and Caspase 9 cleavage are upregulated in a dose-dependent manner[1].

Western Blot Analysis[1]

Cell Line: MCF7 cells
Concentration: 20 μM, 40 μM, 100 μM
Incubation Time: 24 hours
Result: Showed an increase in levels of gH2AX foci and protein.
In Vivo

SCR7 (SCR7 pyrazine; 10 mg/kg; intraperitoneal injection; six doses; BALB/c mice) treatment significantly reduces breast adenocarcinoma-induced tumor and increases lifespan[1].

Animal Model: BALB/c mice injected with breast adenocarcinoma cells[1]
Dosage: 10 mg/kg
Administration: Intraperitoneal injection; on alternate days (0, 2, 4, 6, 8, and 10)
Result: Significantly reduced breast adenocarcinoma-induced tumor and increased lifespan.
Molecular Weight

334.39

Formula

C₁₈H₁₄N₄OS

CAS No.

1533426-72-0

SMILES

O=C(C(/N=C/C1=CC=CC=C1)=C(/N=C/C2=CC=CC=C2)N3)NC3=S.[(E)].[(E)]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

DMSO : ≥ 45 mg/mL (134.57 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9905 mL 14.9526 mL 29.9052 mL
5 mM 0.5981 mL 2.9905 mL 5.9810 mL
10 mM 0.2991 mL 1.4953 mL 2.9905 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (7.48 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

SCR7SCR 7SCR-7DNA/RNA SynthesisCRISPR/Cas9ApoptosisDNAligase-IVnonhomologousend-joininganticancerphosphorylationCaspase 3Caspase 9genereplacementInhibitorinhibitorinhibit

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SCR7
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HY-12742
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