SRI-011381 hydrochloride
Based on 58 publication(s) in Google Scholar
SRI-011381 hydrochloride is an orally active TGF-β signaling agonist, exhibits neuroprotective effects, with blood-brain barrier permeability.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 99.94%
- CAS. Nr.: 2070014-88-7
- Formel: C20H32ClN3O
- Molecular Weight:365.94
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Speicherung:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
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Help & FAQs
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Immunology/Inflammation Compound Library
HY-L007
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Stem Cell Signaling Compound Library
HY-L017
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TGF-beta/Smad Compound Library
HY-L018
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Wnt/Hedgehog/Notch Compound Library
HY-L020
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Anti-Cancer Compound Library
HY-L025
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CNS-Penetrant Compound Library
HY-L028
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Anti-Aging Compound Library
HY-L034
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Differentiation Inducing Compound Library
HY-L038
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Orally Active Compound Library
HY-L061
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Neuroprotective Compound Library
HY-L070
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Angiogenesis-Related Compound Library
HY-L088
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Transcription Factor-Targeted Library
HY-L090
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Targeted Diversity Library
HY-L099
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Anti-Liver Cancer Compound Library
HY-L101
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Osteogenesis Compound Library
HY-L131
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Heterocyclic Compound Library
HY-L138
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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Cell Proliferation Compound Library
HY-L201
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High-Throughput Bioactive Compound Library
HY-L205
Publications Citing Use of MedChemExpress (MCE) SRI-011381 hydrochloride
More- Nature. 2023 Jan;613(7942):120-129. [Abstract]
- Theranostics. 2021 Jul 25;11(17):8480-8499. [Abstract]
- Int J Oral Sci. 2026 Mar 12;18(1):30. [Abstract]
- Cell Mol Biol Lett. 2023 Feb 27;28(1):15. [Abstract]
- Int J Biol Sci. 2018 Oct 31;14(13):1923-1934. [Abstract]
- Sci China Life Sci. 2025 Mar;68(3):746-763. [Abstract]
- Cell Commun Signal. 2023 Jul 4;21(1):168. [Abstract]
- Phytomedicine. 2022 Aug:103:154234. [Abstract]
- Phytomedicine. 2022 Mar 21;100:154067. [Abstract]
- Free Radic Biol Med. 2025 Apr:231:178-192. [Abstract]
- Biomed Pharmacother. 2025 May 1:187:118094. [Abstract]
- Int J Pharm X. 2024 Nov 4:8:100300. [Abstract]
- Phytother Res. 2023 May;37(5):1771-1786. [Abstract]
- J Agric Food Chem. 2022 Sep 7;70(35):10782-10793. [Abstract]
- Cancer Cell Int. 2022 May 16;22(1):191. [Abstract]
- Cell Prolif. 2025 Mar;58(3):e13765. [Abstract]
- Neurobiol Dis. 2024 Jun 18:199:106573. [Abstract]
- J Ethnopharmacol. 2024 Oct 1;337(Pt 2):118876. [Abstract]
- J Ethnopharmacol. 2024 Mar 25:322:117507. [Abstract]
- Life Sci. 2021 Aug 15;279:119359. [Abstract]
- Int J Mol Sci. 2025 Jan 4;26(1):395. [Abstract]
- Int J Mol Sci. 2024 Nov 2;25(21):11778. [Abstract]
- Int Immunopharmacol. 2026 Apr 1:174:116346. [Abstract]
- Eur J Pharmacol. 2026 Feb 15:1015:178549. [Abstract]
- Am J Physiol Cell Physiol. 2025 Jul 1;329(1):C170-C182. [Abstract]
- Eur J Pharmacol. 2021 Oct 5;908:174356. [Abstract]
- Biosci Rep. 2020 Nov 27;40(11):BSR20201545. [Abstract]
- Br Food J. 2025 Jun 11;127(8):2759–2776.
- Biochim Biophys Acta Mol Basis Dis. 2025 Jan 28:167696. [Abstract]
- J Cell Mol Med. 2024 Nov;28(21):e70199. [Abstract]
- J Cell Mol Med. 2021 Apr;25(7):3272-3283. [Abstract]
- Drug Dev Res. 2019 Nov;80(7):992-999. [Abstract]
- J Funct Foods. 2021 May 26.
- Sci Rep. 2024 Oct 15;14(1):24091. [Abstract]
- Chem Res Toxicol. 2022 Feb 21;35(2):233-243. [Abstract]
- Mol Cell Biochem. 2019 Jun;456(1-2):95-104. [Abstract]
- Stem Cells. 2024 May 8:sxae032. [Abstract]
- Mol Med Rep. 2021 Nov;24(5):758. [Abstract]
- Cell Cycle. 2021 Feb;20(3):256-270. [Abstract]
- Int J Med Sci. 2025 Mar 31;22(9):2031-2039. [Abstract]
- Pathol Res Pract. 2025 Mar 23:269:155899. [Abstract]
- Front Biosci (Landmark Ed). 2025 Oct 29;30(10):45595. [Abstract]
- Cell Immunol. 2024 Jan-Feb:395-396:104781. [Abstract]
- Exp Eye Res. 2019 May:182:44-56. [Abstract]
- Dev Comp Immunol. 2018 Nov:88:144-151. [Abstract]
- Biomed Res Int. 2020 Nov 16;2020:8039840. [Abstract]
- Biochem Biophys Res Commun. 2026 Apr 9:808:153453. [Abstract]
- Oncol Lett. 2024 May 30;28(2):346. [Abstract]
- Oncol Lett. 2024 Mar 26;27(5):227. [Abstract]
- Discov Med. 2025 Jun 30.
- Laryngoscope. 2024 Sep;134(9):4078-4087. [Abstract]
- Reprod Domest Anim. 2023 Feb;58(2):323-332. [Abstract]
- Biochem Genet. 2025 Jul 1. [Abstract]
- J Cardiothorac Surg. 2024 May 10;19(1):283. [Abstract]
- Curr Med Sci. 2019 Aug;39(4):534-540. [Abstract]
- Evid Based Complement Alternat Med. 2023 Jan 12:2023:6018375. [Abstract]
- Dis Markers. 2022 May 7;2022:9230647. [Abstract]
- Research Square Preprint. 2020 Dec.
Customer Validation & Images
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WB
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Cell Proliferation/Viability Assay
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RT-PCR
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IF
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Biologische Aktivität
TGF-beta[1]
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:YAPGFAP-CKO mice bearing experimental autoimmune encephalomyelitis (EAE)[2]
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Dosage:30 mg/kg
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Administration:Intraperitoneally injection; every 2 d; for 22 days
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Result:Significantly inhibited inflammatory infiltration and relieved the loss of neurons in YAPGFAP-CKO EAE mice.
Chemical Information
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CAS. Nr. 2070014-88-7
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Appearance Solid
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Molecular Weight 365.94
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Formel C20H32ClN3O
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Color White to off-white
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SMILES
O=C(NC1CCCCC1)N(CC2=CC=CC=C2)CC3CCNCC3.Cl[H]
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (58)
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Journal Impact Factor
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Most Recent
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Nature
2023 Jan;613(7942):120-129. PMID: 36517604 -
Theranostics
Astrocytic YAP protects the optic nerve and retina in an experimental autoimmune encephalomyelitis model through TGF-β signaling. [Abstract]2021 Jul 25;11(17):8480-8499. PMID: 34373754 -
Int J Oral Sci
Gli2 and Gli3 synergistically mediate HH-TGF-β crosstalk in mesenchymal progenitor cells to orchestrate tooth root morphogenesis. [Abstract]2026 Mar 12;18(1):30. PMID: 41813653 -
Cell Mol Biol Lett
IRE1α arm of unfolded protein response in muscle-specific TGF-β signaling-mediated regulation of muscle cell immunological properties. [Abstract]2023 Feb 27;28(1):15. PMID: 36849929 -
Int J Biol Sci
Saikosaponin A Protects From Pressure Overload-Induced Cardiac Fibrosis via Inhibiting Fibroblast Activation or Endothelial Cell EndMT. [Abstract]2018 Oct 31;14(13):1923-1934. PMID: 30443195
SRI-011381 hydrochloride purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2018 Oct 31;14(13):1923-1934. [Abstract]
CFs were stimulated with TGFβ for 24 h and then treated with SSA (30 μM) and SRI-011381 for 12 h, Immunofluorescence staining of α-SMA.
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Sci China Life Sci
Integrating spatial transcriptomics and single-nucleus RNA-seq revealed the specific inhibitory effects of TGF-β on intramuscular fat deposition. [Abstract]2025 Mar;68(3):746-763. PMID: 39422812 -
Cell Commun Signal
Myofiber directs macrophages IL-10-Vav1-Rac1 efferocytosis pathway in inflamed muscle following CTX myoinjury by activating the intrinsic TGF-β signaling. [Abstract]2023 Jul 4;21(1):168. PMID: 37403092 -
Phytomedicine
Modified Shenlingbaizhu Decoction represses the pluripotency of colorectal cancer stem cells by inhibiting TGF-β mediated EMT program. [Abstract]2022 Aug:103:154234. PMID: 35689903 -
Phytomedicine
2022 Mar 21;100:154067. PMID: 35349832 -
Free Radic Biol Med
MSC exosomes and MSC exosomes loaded with LncRNA H19 as nanotherapeutics regulate the neurogenetic potential of Müller Glial Cells in dry age-related macular degeneration. [Abstract]2025 Apr:231:178-192. PMID: 40015462 -
Biomed Pharmacother
Transforming growth factor-β receptor I kinase plays a crucial role in oligodendrocyte regeneration after demyelination. [Abstract]2025 May 1:187:118094. PMID: 40315672 -
Int J Pharm X
Ultrasound-targeted sirolimus-loaded microbubbles improves acute rejection of heart transplantation in rats by inhibiting TGF-β1-Smad signaling pathway, promoting autophagy and reducing inflammation. [Abstract]2024 Nov 4:8:100300. PMID: 39624342 -
Phytother Res
Asiaticoside hampers epithelial-mesenchymal transition by promoting PPARG expression and suppressing P2RX7-mediated TGF-β/Smad signaling in triple-negative breast cancer. [Abstract]2023 May;37(5):1771-1786. PMID: 36444395 -
J Agric Food Chem
Glabridin from Glycyrrhiza glabra Possesses a Therapeutic Role against Keloid via Attenuating PI3K/Akt and Transforming Growth Factor-β1/SMAD Signaling Pathways. [Abstract]2022 Sep 7;70(35):10782-10793. PMID: 36005946 -
Cancer Cell Int
Silencing of histone deacetylase 3 suppresses the development of esophageal squamous cell carcinoma through regulation of miR-494-mediated TGIF1. [Abstract]2022 May 16;22(1):191. PMID: 35578338 -
Cell Prolif
2025 Mar;58(3):e13765. PMID: 39407407
SRI-011381 hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2025 Mar;58(3):e13765. [Abstract]
qRT‐PCR confirmed the relative expression of foxj1a and its target genes in WT or gdf11 mutants treated with SRI‐011381 (0.2 μM).
SRI-011381 hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2025 Mar;58(3):e13765. [Abstract]
SRI‐011381 (0.2 μM) treatment restored cilia morphology in the mutants.
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Neurobiol Dis
Role of oxidative phosphorylation in the antidepressant effects of arketamine via the vagus nerve-dependent spleen-brain axis. [Abstract]2024 Jun 18:199:106573. PMID: 38901783 -
J Ethnopharmacol
The fruit of Rosa odorata sweet var. gigantea (Coll. et Hemsl.) Rehd. et Wils attenuates chronic atrophic gastritis induced by MNNG and its potential mechanism. [Abstract]2024 Oct 1;337(Pt 2):118876. PMID: 39362325 -
J Ethnopharmacol
The mechanism of Shenlong Jianji treatment of idiopathic pulmonary fibrosis inhibits fibroblast-to-myofibroblast transformation via the TGF-β1/smads signaling pathway. [Abstract]2024 Mar 25:322:117507. PMID: 38122910 -
Life Sci
Synergistic efficacy of curcumin and anti-programmed cell death-1 in hepatocellular carcinoma. [Abstract]2021 Aug 15;279:119359. PMID: 33753114
SRI-011381 hydrochloride purchased from MedChemExpress. Usage Cited in: Life Sci. 2021 Aug 15;279:119359. [Abstract]
The expression of pSmad2/3 in PBMCs treated with Curcumin or SRI-011381 (10 μM) detected by Western blot analysis normalized to GAPDH. SRI-011381 is found to up-regulate the phosphorylation of Smad2/3, while Curcumin reduces the activation of Smad2/3, whereas the inhibitory effect of curcumin in the activation of Smad2/3 is reversed by SRI-011381.
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Int J Mol Sci
Mechanism of THBS1 Regulation of MDCK Cell Proliferation and Apoptosis Through TGF-β/Smad Signalling. [Abstract]2025 Jan 4;26(1):395. PMID: 39796249
SRI-011381 hydrochloride purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Jan 4;26(1):395. [Abstract]
Effect of different concentrations of SRI-011381 (2 μg/mL, 5 μg/mL, 10 μg/mL) on THBS1-sh-122484 cells and different concentrations of LY2109761 on THBS1-OE cells TGF-β/Smad signalling downstream target genes and THBS1 protein level expression differences.
SRI-011381 hydrochloride purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Jan 4;26(1):395. [Abstract]
Effect of different concentrations of SRI-011381 (2, 5, 10, 10, 50 μg/mL) on the growth status of THBS1-sh-122484 cells.
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Int J Mol Sci
Loss of DNA Polymerase β Delays Atherosclerosis in ApoE-/- Mice Due to Inhibition of Vascular Smooth Muscle Cell Migration. [Abstract]2024 Nov 2;25(21):11778. PMID: 39519329 -
Int Immunopharmacol
Milk exosomes loaded with atorvastatin suppress fibroblast activation and extracellular matrix remodeling via the TGF-β/SMAD pathway in ligamentum flavum hypertrophy. [Abstract]2026 Apr 1:174:116346. PMID: 41707594 -
Eur J Pharmacol
Hirsutine attenuates renal injury in diabetic kidney disease by regulating Th17 cell differentiation via the TGFβ/SMAD Pathway. [Abstract]2026 Feb 15:1015:178549. PMID: 41544688 -
Am J Physiol Cell Physiol
THBS4 downregulation alters trophoblast function in preeclampsia via the TGF-β1/Smad signaling cascade. [Abstract]2025 Jul 1;329(1):C170-C182. PMID: 40478535 -
Eur J Pharmacol
2021 Oct 5;908:174356. PMID: 34280398 -
Biosci Rep
miR-200-3p suppresses cell proliferation and reduces apoptosis in diabetic retinopathy via blocking the TGF-β2/Smad pathway. [Abstract]2020 Nov 27;40(11):BSR20201545. PMID: 33150936 -
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Biochim Biophys Acta Mol Basis Dis
Programmed cell death protein 5 inhibits hepatocellular carcinoma progression by inducing pyroptosis through regulation of TGF-β/Smad2/3/Snail pathway. [Abstract]2025 Jan 28:167696. PMID: 39884472 -
J Cell Mol Med
Fibroblast-derived miR-425-5p alleviates cardiac remodelling in heart failure via inhibiting the TGF-β1/Smad signalling. [Abstract]2024 Nov;28(21):e70199. PMID: 39527465 -
J Cell Mol Med
Metformin attenuates post-epidural fibrosis by inhibiting the TGF-β1/Smad3 and HMGB1/TLR4 signaling pathways. [Abstract]2021 Apr;25(7):3272-3283. PMID: 33611840 -
Drug Dev Res
Curcumin mitigates the epithelial-to-mesenchymal transition in biliary epithelial cells through upregulating CD109 expression. [Abstract]2019 Nov;80(7):992-999. PMID: 31403228 -
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Sci Rep
Oroxylin A suppressed colorectal cancer metastasis by inhibiting the activation of the TGF-β/SMAD signal pathway. [Abstract]2024 Oct 15;14(1):24091. PMID: 39406881 -
Chem Res Toxicol
Advancing the Adverse Outcome Pathway for PPARγ Inactivation Leading to Pulmonary Fibrosis Using Bradford-Hill Consideration and the Comparative Toxicogenomics Database. [Abstract]2022 Feb 21;35(2):233-243. PMID: 35143163 -
Mol Cell Biochem
Knockdown of KDM2A inhibits proliferation associated with TGF-β expression in HEK293T cell. [Abstract]2019 Jun;456(1-2):95-104. PMID: 30604066 -
Stem Cells
Tibetan Mesenchymal Stem Cell-derived Exosomes Alleviate Pulmonary Vascular Remodeling in Hypoxic Pulmonary Hypertension Rats. [Abstract]2024 May 8:sxae032. PMID: 38717187 -
Mol Med Rep
Exosomes from miR-29a-modified adipose-derived mesenchymal stem cells reduce excessive scar formation by inhibiting TGF-β2/Smad3 signaling. [Abstract]2021 Nov;24(5):758. PMID: 34476508
SRI-011381 hydrochloride purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2021 Nov;24(5):758. [Abstract]
Mimics-exo significantly inhibits the expression levels of TGF-β2 and p-Smad3, as well as downregulated the expression levels of Col-I, Col-III and α-SMA, while SRI-011381 hydrochloride (10 µM) reverses the inhibition of miR-29a-overexpressing hADSCs-exo on the activation of TGF-β2/Smad3 signaling and the fibrosis of HSFBs.
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Cell Cycle
Bone mesenchymal stem cell-derived extracellular vesicles promote the repair of intervertebral disc degeneration by transferring microRNA-199a. [Abstract]2021 Feb;20(3):256-270. PMID: 33499725 -
Int J Med Sci
Modulation of Chemotherapy Sensitivity of Breast Cancer Cells through Transforming Growth Factor-beta Pathway-mediated Alterations in DNA Damage Response. [Abstract]2025 Mar 31;22(9):2031-2039. PMID: 40303493 -
Pathol Res Pract
2025 Mar 23:269:155899. PMID: 40168772 -
Front Biosci (Landmark Ed)
Astragalus Polysaccharide Improves Myocardial Fibrosis in Hypertrophic Cardiomyopathy Through the TGF-β1/Smad3 Signal Pathway. [Abstract]2025 Oct 29;30(10):45595. PMID: 41198559 -
Cell Immunol
Dexamethasone alleviates pulmonary sarcoidosis by regulating the TGF-β/Smad3 signaling to promote Th17/Treg cell rebalance. [Abstract]2024 Jan-Feb:395-396:104781. PMID: 38159414 -
Exp Eye Res
Expansion and maintenance of primary corneal epithelial stem/progenitor cells by inhibition of TGFβ receptor I-mediated signaling. [Abstract]2019 May:182:44-56. PMID: 30914160 -
Dev Comp Immunol
Identification and functional analysis of transforming growth factor-β type I receptor (TβR1) from Scylla paramamosain: The first evidence of TβR1 involved in development and innate immunity in crustaceans. [Abstract]2018 Nov:88:144-151. PMID: 30031868 -
Biomed Res Int
Inhibition of Wilms' Tumor Proliferation and Invasion by Blocking TGF- β Receptor I in the TGF- β/Smad Signaling Pathway. [Abstract]2020 Nov 16;2020:8039840. PMID: 33282954 -
Biochem Biophys Res Commun
Macrophage erythropoietin receptor signaling drives macrophage-myofibroblast transition via JAK2/STAT3 and TGF-β1/Smad3 pathways to exacerbate pulmonary fibrosis in mice. [Abstract]2026 Apr 9:808:153453. PMID: 41719615 -
Oncol Lett
Biological effect of ETV4 and the underlying mechanism of its regulatory effect on epithelial‑mesenchymal transition in intrahepatic cholangiocarcinoma cells. [Abstract]2024 May 30;28(2):346. PMID: 38872859 -
Oncol Lett
2024 Mar 26;27(5):227. PMID: 38586208 -
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Laryngoscope
POSTN Regulates Fibroblast Proliferation and Migration in Laryngotracheal Stenosis Through the TGF-β/RHOA Pathway. [Abstract]2024 Sep;134(9):4078-4087. PMID: 38771155 -
Reprod Domest Anim
Sirtuin 7 is essential for the survival and synthesis of estrogen in Yak (Bos grunniens) cumulus granulosa cells. [Abstract]2023 Feb;58(2):323-332. PMID: 36305218 -
Biochem Genet
Silencing THBS1 in M2 Macrophages Exerts an Inhibitory Effect on Tongue Squamous Cell Carcinoma by Suppressing TGF-β Pathway. [Abstract]2025 Jul 1. PMID: 40591142 -
J Cardiothorac Surg
Interval training suppresses nod-like receptor protein 3 inflammasome activation to improve cardiac function in myocardial infarction rats by hindering the activation of the transforming growth factor-β1 pathway. [Abstract]2024 May 10;19(1):283. PMID: 38730417 -
Curr Med Sci
Bcl6 Suppresses Cardiac Fibroblast Activation and Function via Directly Binding to Smad4. [Abstract]2019 Aug;39(4):534-540. PMID: 31346987 -
Evid Based Complement Alternat Med
The Carthamus tinctorius L. and Lepidium apetalum Willd. Drug Pair Inhibits EndMT through the TGF β 1/Snail Signaling Pathway in the Treatment of Myocardial Fibrosis. [Abstract]2023 Jan 12:2023:6018375. PMID: 36686974 -
Dis Markers
Iodine-125 Seeds Inhibit Carcinogenesis of Hepatocellular Carcinoma Cells by Suppressing Epithelial-Mesenchymal Transition via TGF- β 1/Smad Signaling. [Abstract]2022 May 7;2022:9230647. PMID: 35578690 -
Lösungsmittel & Löslichkeit
DMSO : 33.33 mg/mL (91.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 25 mg/mL (68.32 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.83 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.83 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 50 mg/mL (136.63 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Reinheit & Dokumentation
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Data Sheet (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Jingyin Han, et al. The Improvement Effect of Sodium Ferulate on the Formation of Pulmonary Fibrosis in Silicosis Mice Through the Neutrophil Alkaline Phosphatase 3 (NALP3)/Transforming Growth Factor-β1 (TGF-β1)/α-Smooth Muscle Actin (α-SMA) Pathway. Med Sci Monit. 2021 Jun 15;27:e927978. [Content Brief]
[2]. Qian Wu, et al. Astrocytic YAP protects the optic nerve and retina in an experimental autoimmune encephalomyelitis model through TGF-β signaling. Theranostics. 2021 Jul 25;11(17):8480-8499. [Content Brief]
[3]. Luo J. TGF-β as a Key Modulator of Astrocyte Reactivity: Disease Relevance and Therapeutic Implications. Biomedicines. 2022 May 23;10(5):1206. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.7327 mL | 13.6634 mL | 27.3269 mL | 68.3172 mL |
| 5 mM | 0.5465 mL | 2.7327 mL | 5.4654 mL | 13.6634 mL | |
| 10 mM | 0.2733 mL | 1.3663 mL | 2.7327 mL | 6.8317 mL | |
| 15 mM | 0.1822 mL | 0.9109 mL | 1.8218 mL | 4.5545 mL | |
| 20 mM | 0.1366 mL | 0.6832 mL | 1.3663 mL | 3.4159 mL | |
| 25 mM | 0.1093 mL | 0.5465 mL | 1.0931 mL | 2.7327 mL | |
| 30 mM | 0.0911 mL | 0.4554 mL | 0.9109 mL | 2.2772 mL | |
| 40 mM | 0.0683 mL | 0.3416 mL | 0.6832 mL | 1.7079 mL | |
| 50 mM | 0.0547 mL | 0.2733 mL | 0.5465 mL | 1.3663 mL | |
| 60 mM | 0.0455 mL | 0.2277 mL | 0.4554 mL | 1.1386 mL | |
| DMSO | 80 mM | 0.0342 mL | 0.1708 mL | 0.3416 mL | 0.8540 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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