Suc-AAPF-pNA
Based on 2 publication(s) in Google Scholar
Suc-AAPF-pNA (Suc-Ala-Ala-Pro-Phe-pNA) is a chromogenic p-nitroanilide (pNA) substrate with the Km of 1.7 mM. Cleavage of Suc-AAPF-pNA releases 4-nitroaniline, which is yellow in colour and can be measured spectrophotometrically. Suc-AAPF-pNA can be used for the measurement of free and membrane-bound cathepsin G in human neutrophils.
For research use only. We do not sell to patients.
- Purity: 99.55%
- CAS No.: 70967-97-4
- Formula: C30H36N6O9
- Molecular Weight:624.64
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Storage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Suc-AAPF-pNA
More
Customer Validation & Images
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
Biological Activity
The elastase activity is measured as activity against the substrate Suc-AAPF-Pna. Aliquots (10 μL) of SmCTF samples are incubated at 30 °C with 200 μM Suc-AAPF-pNA and the activity of cercarial elastase measured as abosorbance at 400 nm[2].
Proteolytic activity is measured by hydrolysis of the substrate suc-AAPF-pNA. Serial dilutions of the proteases are made in 100 mM Tris buffer, pH 8.6, containing 10 mM CaCl2. Enzyme activity is measured at 25°C in 100 mM Tris/HCl, pH 8.6 containing 1.6 mM suc-AAPF-pNA, 0.0005% Tween-80 and 1% DMSO by monitoring the absorbance change at 410 nm using a spectrophotometer. Each dilution of enzyme is tested in triplicate[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
{Suc}-Ala-Ala-Pro-Phe-{pNA}
Suc-AAPF-pNA
Chemical Information
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CAS No. 70967-97-4
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Appearance Solid
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Molecular Weight 624.64
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Formula C30H36N6O9
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Color White to off-white
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Synonyms
Suc-Ala-Ala-Pro-Phe-pNA
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Publications (2)
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Journal Impact Factor
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Most Recent
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Int J Biol Macromol
Dynamic catalytic domain plasticity governs substrate specificity in industrial serine proteases: Structural and functional implications. [Abstract]2025 Oct 17;331(Pt 1):148374. PMID: 41110577
Suc-AAPF-pNA purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Oct 17;331(Pt 1):148374. [Abstract]
Predicted binding free energies and interaction modes between the three proteases and various substrates (Suc-AAPA-pNA, Suc-AAPI-pNA, Suc-AAPL-pNA and Suc-AAPF-pNA).aUnits: kcal/mol.
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Biochem Pharmacol
2025 Oct 23:117451. PMID: 41138926
Suc-AAPF-pNA purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2025 Oct 23:117451. [Abstract]
HEK293 cells were pretreated with Suc-AAPF-pNA (5 uM) and then stimulated with MRGPRX2 agonist C48/80 (30 ug/mL). The fluorescence intensity was recorded for 2 min. Suc-AAPF-pNA (5 uM) exhibited inhibitory effects on MRGPRX2-mediated calcium mobilization.
Solvent & Solubility
DMSO : 100 mg/mL (160.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.00 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.00 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Sylvie Attucci, et al. Measurement of free and membrane-bound cathepsin G in human neutrophils using new sensitive fluorogenic substrates. Biochem J. 2002 Sep 15;366(Pt 3):965-70. [Content Brief]
[2]. A J Webb, et al. A protease-based biosensor for the detection of schistosome cercariae. Sci Rep. 2016 Apr 19:6:24725. [Content Brief]
[3]. Jeanette M Mucha, et al. Enhanced immunogenicity of a functional enzyme by T cell epitope modification. BMC Immunol. 2002:3:2. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6009 mL | 8.0046 mL | 16.0092 mL | 40.0231 mL |
| 5 mM | 0.3202 mL | 1.6009 mL | 3.2018 mL | 8.0046 mL | |
| 10 mM | 0.1601 mL | 0.8005 mL | 1.6009 mL | 4.0023 mL | |
| 15 mM | 0.1067 mL | 0.5336 mL | 1.0673 mL | 2.6682 mL | |
| 20 mM | 0.0800 mL | 0.4002 mL | 0.8005 mL | 2.0012 mL | |
| 25 mM | 0.0640 mL | 0.3202 mL | 0.6404 mL | 1.6009 mL | |
| 30 mM | 0.0534 mL | 0.2668 mL | 0.5336 mL | 1.3341 mL | |
| 40 mM | 0.0400 mL | 0.2001 mL | 0.4002 mL | 1.0006 mL | |
| 50 mM | 0.0320 mL | 0.1601 mL | 0.3202 mL | 0.8005 mL | |
| 60 mM | 0.0267 mL | 0.1334 mL | 0.2668 mL | 0.6671 mL | |
| 80 mM | 0.0200 mL | 0.1001 mL | 0.2001 mL | 0.5003 mL | |
| 100 mM | 0.0160 mL | 0.0800 mL | 0.1601 mL | 0.4002 mL |
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