Préparation d'échantillons de protéines

Lyse cellulaire (6)

Cat. No.: HY-K1001 RIPA Lysis Buffer (Strong)

MCE RIPA Lysis Buffer is one of the most reliable buffers used to lyse cells from both cultured cells and tissues.

Cat. No.: HY-K1002 NP-40 Lysis Buffer

MCE NP-40 Lysis Buffer is a relatively mild reliable buffers used to lyse cells from animal, plant tissue and fungi, bacteria etc.

Cat. No.: HY-K1003 Mammalian Active Protein Extraction Reagent

MCE Mammalian Active Protein Extraction Reagent are gentle, non-denaturing detergents designed for the rapid, high-quality, and high-activity extraction of cytoplasmic, nuclear, and membrane proteins from mammalian cells or tissues. The 100 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K1000 WB/IP Lysis Buffer

MCE WB/IP Lysis Buffer is a lysis buffer used to lyse cell or tissue samples under non-denaturing conditions to prepare protein samples. The lysed cell or tissue samples can be used for PAGE, Western Blot, Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP), ELISA, and other assays.

Cat. No.: HY-K3010 Red Blood Cell Lysis Buffer (10×)

MCE Red Blood Cell Lysis Buffer (10×) primarily contains ammonium chloride and is a ready-to-use solution designed for rapid and effective lysis and removal of anucleated red blood cells from human or mouse blood and tissue samples without affecting white blood cells, normal tissues, or tumor cells.

Cat. No.: HY-K0002 Bacterial Protein Extraction Reagent

MCE Bacterial Protein Extraction Reagent integrates both lysozyme and nuclease activities and is specifically formulated for E. coli lysis. It efficiently disrupts the peptidoglycan layer under mild conditions to rapidly release intracellular proteins. Simultaneously, the incorporated nucleases degrade genomic DNA/RNA, significantly reducing lysate viscosity and minimizing nucleic-acid interference, thereby preserving the native conformation and functional integrity of target proteins to the greatest extent.

Tampon de lavage (3)

Cat. No.: HY-K1023 PBS Powder (1 L of 1×)

MCE PBS Powder (1 L of 1×) consists of NaCl, KCl and Phosphate, and is widely used in immunoassay, microbiology and protein experiments. The 20 pouches are defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K1024 PBS Powder (2 L of 1×)

MCE PBS Powder (2 L of 1×) consists of NaCl, KCl and Phosphate, and is widely used in immunoassay, microbiology and protein experiments. The 10 pouches are defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K1026 TBS Powder (1 L of 1×)

MCE TBS Powder (1 L of 1×) consists of NaCl, KCl and Tris, and is widely used as washing buffer in immunocytochemistry, FISH or ELISA, and can also be used for antibody dilution and preparation of blocking solution. The 20 pouches are defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cocktail d'inhibiteurs de protéase (5)

Cat. No.: HY-K0010 Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO)

MCE Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) is a blend of 6 pan-protease inhibitors for protection of protein integrity.The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0011 Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free)

MCE Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) is a blend of 5 pan-protease inhibitors for protection of protein integrity. The 25 pcs is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0012 Protease Inhibitor Cocktail, Bacteria (EDTA-Free, 100× in DMSO)

MCE Protease Inhibitor Cocktail, Bacteria (EDTA-Free, 100× in DMSO) protects proteins from degradation by endogenous proteases released during protein extraction and purification.The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0013 Protease and Phosphatase Inhibitor Cocktail (EDTA-Free, 10× in ddH2O)

MCE Protease and Phosphatase Inhibitor Cocktail (EDTA-Free, 10× in ddH2O) protects protein from degradation by endogenous proteases released during protein extraction and purification.The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0004 PMSF (100×)

MCE PMSF (100×) is a ready-to-use protease inhibitor solution. It can be added directly to lysis buffers at a 1:100 dilution before use.

Cocktail d'inhibiteurs de phosphatase (3)

Cat. No.: HY-K0021 Phosphatase Inhibitor Cocktail I (100× in DMSO)

Phosphatase Inhibitor Cocktail I (100× in DMSO) is a blend of 2 phosphatase inhibitors that can effectively inhibit alkaline phosphatases and serine/threonine phosphatases. It is used to maintain the phosphorylation status of proteins.The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0022 Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)

Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) is a blend of 5 phosphatase inhibitors that can broadly inhibit acid phosphatases, alkaline phosphatases, and protein tyrosine phosphatases (PTPs).The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0023 Phosphatase Inhibitor Cocktail Ⅲ (100× in DMSO)

Phosphatase Inhibitor Cocktail Ⅲ (100× in DMSO) is a mixture of multiple phosphatase inhibitors that effectively inhibit serine/threonine phosphatases and alkaline phosphatases, and is used to preserve the phosphorylation status of proteins.The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cocktail d'inhibiteurs de désacétylase (1)

Cat. No.: HY-K0030 Deacetylase Inhibitor Cocktail (100× in 70% DMSO)

MCE Deacetylase Inhibitor Cocktail is a synergistic combination of chemicals designed to preserve the acetylation state of proteins.The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cocktail d'inhibiteurs de kinase (2)

Cat. No.: HY-K0016 Kinase Assay Buffer

MCE Kinase Assay Buffer can be used for assaying kinase protein. The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0017 Kinase Inhibitor Cocktail

MCE Kinase Inhibitor Cocktail controls the biological activity of kinases. This inhibitor is primarily used for Biochemicals applications.The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Dosage des protéines (2)

Cat. No.: HY-K2001 Bradford Protein Assay Kit

MCE Bradford Protein Assay Kit is suitable for detecting the total protein content in samples such as serum, plasma, animal tissues, and cells.

Cat. No.: HY-K2002 BCA Protein Assay Kit

MCE BCA Protein Assay Kit is a colorimetric assay based on the bicinchoninic acid (BCA) reaction principle for quantitative protein determination.