One- and two-photon live cell imaging using a mutant SNAP-Tag protein and its FRET substrate pairs

  • Org Lett. 2011 Aug 19;13(16):4160-3. doi: 10.1021/ol201430x.
Chong-Jing Zhang  1 ,  Lin Li ,  Grace Y J Chen ,  Qing-Hua Xu ,  Shao Q Yao
Affiliations
  • 1. Department of Chemistry, National University of Singapore, Singapore 117543.
PMID: 21786765 DOI: 10.1021/ol201430x
Abstract

A small molecule-assisted protein labeling strategy based on a mutant SNAP-Tag (mSNAP) and its FRET substrate pairs has been developed. Both one- and two-photon fluorescence microscopic experiments were successfully demonstrated in living cells.

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