Characterization of chlorinated valepotriates from Valeriana jatamansi

  • Phytochemistry. 2013 Jan;85:185-93. doi: 10.1016/j.phytochem.2012.08.015.
Sheng Lin  1 ,  Zhong-Xiao Zhang ,  Tao Chen ,  Ji Ye ,  Wei-Xing Dai ,  Lei Shan ,  Juan Su ,  Yun-Heng Shen ,  Hui-Liang Li ,  Run-Hui Liu ,  Xi-ke Xu ,  Hui Wang ,  Wei-dong Zhang
Affiliations
  • 1. Department of Natural Product Chemistry, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, People's Republic of China.
PMID: 23036722 DOI: 10.1016/j.phytochem.2012.08.015
Abstract

HPLC-PDA-MS and TLC analysis were used to look for minor cytotoxic chlorinated valepotriates from whole Plants of Valeriana jatamansi (syn. Valeriana wallichii DC.). This resulted in isolation of 15 chlorinated valepotriates, designated as chlorovaltrates A-O, together with six known analogues, (1S,3R,5R,7S,8S,9S)-3,8-epoxy-1,5-dihydroxyvalechlorine, volvaltrate B, chlorovaltrate, rupesin B, (1S,3R,5R,7S,8S,9S)-3,8-epoxy-1-O-ethyl-5-hydroxyvalechlorine, and (1R,3R,5R,7S,8S,9S)-3,8-epoxy-1-O-ethyl-5-hydroxyvalechlorine. Their structures were elucidated by spectroscopic methods including homo- and heteronuclear two-dimensional NMR experiments. Chlorovaltrates K-N, chlorovaltrate and rupesin B showed moderate cytotoxicity against lung adenocarcinoma (A 549), metastatic prostate Cancer (PC-3M), colon Cancer (HCT-8) and hepatoma (Bel 7402) cell lines with IC₅₀ values of 0.89-9.76 μM.

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