NCL - nucleolin Gene

Also Known as C23; Nsr1

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 4691

About NCL

Cytogenetic location: 2q37.1 Genomic coordinates (GRCh38): 2:231,453,531-231,464,484 (from NCBI)

This gene has 24 transcripts (splice variants), 125 orthologues and 36 paralogues. Ubiquitous expression in lymph node (RPKM 147.8), appendix (RPKM 146.7) and 25 other tissues.

Summary

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]

NCL Products (1)

mRNA	Protein	Name
NM_005381.3	NP_005372.2	nucleolin

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables DNA topoisomerase binding	IPI IPI: Inferred from physical interaction	8567649	GOA
enables RNA binding	IDA IDA: Inferred from direct assay	8321232	GOA
enables identical protein binding	IPI IPI: Inferred from physical interaction	21575138	GOA
enables mRNA 5'-UTR binding	IDA IDA: Inferred from direct assay	16213212	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	12147681	GOA
enables telomeric DNA binding	IDA IDA: Inferred from direct assay	8321232	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in angiogenesis	IDA IDA: Inferred from direct assay	16403913	GOA
involved in negative regulation of translation	IMP IMP: Inferred from mutant phenotype	16213212	GOA
involved in positive regulation of transcription of nucleolar large rRNA by RNA polymerase I	IMP IMP: Inferred from mutant phenotype	24071584	GOA

Cellular Component GO Annotation	Evidence	References	Source
located in cell cortex	IDA IDA: Inferred from direct assay	16403913	GOA
located in cytoplasmic ribonucleoprotein granule	IDA IDA: Inferred from direct assay	15121898	GOA
located in nucleolus	IDA IDA: Inferred from direct assay	12944467	GOA
located in nucleoplasm	IDA IDA: Inferred from direct assay	23353999	GOA
located in nucleus	IDA IDA: Inferred from direct assay	15121898	GOA
part of ribonucleoprotein complex	IDA IDA: Inferred from direct assay	17289661	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

NCL Protein Structure

RRM_1

0
200
400
600
710 a.a.

Protein Preferred Names	Protein Names
nucleolin

NCL Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	NCL	P19338	ARFGEF1	Homo sapiens	Q9Y6D6	Anti Bait CoIP	18292223
Intra	NCL	P19338	P39060-PRO_0000005794	Homo sapiens	P39060-PRO_0000005794	Anti Bait CoIP	17615292
Intra	NCL	P19338	P39060-PRO_0000005794	Homo sapiens	P39060-PRO_0000005794	Pull Down	17615292
Intra	NCL	P19338	P39060-PRO_0000005794	Homo sapiens	P39060-PRO_0000005794	SPR	19877579
Intra	NCL	P19338	P39060-PRO_0000005794	Homo sapiens	P39060-PRO_0000005794	FACS	19877579
Intra	NCL	P19338	P39060-PRO_0000005794	Homo sapiens	P39060-PRO_0000005794	SPR	17615292
Intra	NCL	P19338	P39060-PRO_0000005794	Homo sapiens	P39060-PRO_0000005794	Anti Bait CoIP	19877579
Intra	NCL	P19338	PAX8	Homo sapiens	Q06710	Display Tech	20195357
Intra	NCL	P19338	MDM2	Homo sapiens	Q00987	Display Tech	20195357
Intra	NCL	P19338	MDM2	Homo sapiens	Q00987	Pull Down	22103682
Intra	NCL	P19338	YWHAZ	Homo sapiens	P63104	Pull Down	15161933
Intra	NCL	P19338	MYH9	Homo sapiens	P35579	Confocal	16403913
Intra	NCL	P19338	TP53	Homo sapiens	P04637	Far-WB	22103682
Intra	NCL	P19338	TP53	Homo sapiens	P04637	Pull Down	22103682
Intra	NCL	P19338	RHOA	Homo sapiens	P61586	IF	21281639
Intra	NCL	P19338	RHOA	Homo sapiens	P61586	Anti Bait CoIP	21281639
Intra	NCL	P19338	H1-5	Homo sapiens	P16401	Crosslink	30021884
Intra	NCL	P19338	ARRB2	Homo sapiens	P32121	Anti Tag CoIP	17620599
Cross	NCL	P19338	Ankrd1	Mus musculus	Q9CR42	Anti Bait CoIP	24515436
Cross	NCL	P19338	Ankrd1	Mus musculus	Q9CR42	Anti Tag CoIP	24515436
Intra	NCL	P19338	PA2G4	Homo sapiens	Q9UQ80	Pull Down	15064750
Cross	NCL	P19338	P27958-PRO_0000037577	Hepatitis C virus	P27958-PRO_0000037577	Pull Down	12427757
Cross	NCL	P19338	P27958-PRO_0000037577	Hepatitis C virus	P27958-PRO_0000037577	Confocal	12427757

Cross: Cross-species interaction Intra: Intraspecies interaction

Recombinant NCL Proteins

Cat. No.	Product Name	Accession	Purity
HY-P71760	NCL Protein, Human (P.pastoris, His)	P19338 (V2-S482)	≥ 90%, as determined by reducing SDS-PAGE.

NCL Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P80252	Nucleolin Antibody (YA259)	WB, ICC/IF, IHC-P	Human, Mouse, Rat
HY-P810619	Phospho-Nucleolin (Thr76) Antibody (YA9881)	WB, IHC-P	Human
HY-P810620	Phospho-Nucleolin (Thr84) Antibody (YA9882)	WB, IHC-P	Human
HY-P81695	Phospho-Nucleolin (Thr84) Antibody (YA1440)	WB	Human
HY-P81695A	Phospho-Nucleolin (Thr84) Antibody (YA1440)(PBS only)	WB	Human
HY-P86502	Nucleolin Antibody (YA6194)	WB, IHC-P, ICC/IF, IP, ELISA	Human, Mouse

Related Diseases

Diseases	Alias
Cockayne Syndrome	Cockayne'S Syndrome Dwarfism-Retinal Atrophy-Deafness Syndrome Neill-Dingwall Syndrome Progeria-Like Syndrome Progeroid Nanism Cs
Spinal Muscular Atrophy	Sma 5q Sma Proximal Sma Sma-Associated Sma Spinal Amyotrophies Spinal Amyotrophy Spinal Muscle Degeneration Spinal Muscle Wasting Muscular Atrophy Spinal Atrophy, Muscular, Spinal Hereditary Motor Neuronopathy Progressive Muscular Atrophy Sma - [Spinal Muscular Atrophy]
Autoimmune Disease	Autoimmune Diseases Autoimmune Hypersensitivity Disease Hypersensitivity Reaction Type Ii Disease Type Ii Hypersensitivity Reaction Disease
Myelodysplastic Syndrome	Myelodysplastic Syndromes Myelodysplasia MDS Myelodysplastic Syndrome Included Myelodysplastic Syndrome, Susceptibility To, Included Myelodysplastic Syndrome, Somatic Myelodysplastic Syndrome, Susceptibility To
Breast Cancer	Breast Carcinoma Male Breast Cancer Breast Cancer, Familial Malignant Neoplasm Of Breast Breast Cancer, Susceptibility To Breast Cancer, Early-Onset Malignant Tumor Of Breast Carcinoma Of Male Breast Breast Cancer, Invasive Ductal Breast Cancer, Protection Against Breast Cancer, Somatic Breast Cancer, Male Breast Cancer, Lobular, Somatic Breast Tumor Mammary Cancer Mammary Tumor Malignant Neoplasm Of Male Breast Mammary Carcinoma Male Breast Carcinoma Familial Cancer Of Breast Invasive Ductal Breast Carcinoma Breast Cancer Susceptibility Breast Cancer, Male, Susceptibility To Breast Cancer, Early-Onset, Susceptibility To Malignant Tumor Of The Breast Mammary Neoplasm Primary Breast Cancer Neoplasm Of Male Breast Carcinoma Of Breast Breast Cancer In Men Familial Breast Cancer Cancer Of Breast BC Breast Cancer Familial Breast Cancer Familial Male Breast Cancer, Familial Male Breast Male Carcinoma Breast Neoplasms Breast Neoplasms, Male Mammary Tumors Mammary Carcinomas Cancer, Breast Cancer, Breast, Susceptibility Invasive Breast Ductal Carcinoma Breast Neoplasm Susceptibility To Breast Cancer Mammary Neoplasms Animal Mammary Neoplasms Primary Malignant Neoplasm Of Breast Infiltrating Ductal Carcinoma Of Breast Infiltrating Duct Carcinoma Of Unspecified Site Infiltrating Ductular Carcinoma Of Unspecified Site Invasive Breast Carcinoma Of No Special Type Microinvasive Carcinoma Of Breast Carcinoma With Apocrine Differentiation
Systemic Lupus Erythematosus	Lupus Nephritis SLE Disseminated Lupus Erythematosus Systemic Lupus Erythematosus, Susceptibility To Lupus Erythematosus, Systemic Lupus Nephritis, Susceptibility To Libman-Sacks Disease Systemic Lupus Erythematosus Susceptibility To Sle - Lupus Erythematosus, Systemic Le Syndrome Lupus Lupus Erythematosus Systemic Lupus Erythematosus, Systemic, Susceptibility To Lupus Vulgaris Lupus Erythematosus, Discoid Lupus Erythematosus Systemic Lupus Erythematosus Nos Sle - [Systemic Lupus Erythematosus]
Diamond-Blackfan Anemia	Congenital Pure Red Cell Aplasia Aase Syndrome Erythrogenesis Imperfecta Anemia, Diamond-Blackfan Congenital Hypoplastic Anemia Aase-Smith Ii Syndrome Bds Blackfan-Diamond Anemia Congenital Prca Congenital Hypoplastic Anemia, Blackfan-Diamond Type Dba Blackfan - Diamond Syndrome Chronic Constitutional Pure Red Cell Anaemia Anemia Diamond Blackfan Type Anemia Congenital Erythroid Hypoplastic Aregenerative Anemia Chronic Congenital Blackfan Diamond Syndrome Red Cell Aplasia, Pure Hereditary Aase-Smith Syndrome Ii Bda Blackfan Diamond Anemia Blackfan-Diamond Disease Blackfan-Diamond Syndrome Chronic Congenital Agenerative Anemia Congenital Erythroid Hypoplastic Anemia Congenital Hypoplastic Anemia Of Blackfan And Diamond Congenital Pure Red Cell Anemia Hypoplastic Congenital Anemia Inherited Erythroblastopenia Pure Hereditary Red Cell Aplasia Anemia, Hypoplastic, Congenital Anemia Hypoplastic Congenital Fanconi Anemia Constitutional Aplastic Anemia Diamond-Blackfan Anemia 1 Aase Smith Syndrome 2 Congenital Red Cell Aplasia Red Cell Aplasia Of Infants Pure Red Cell Aplasia Of Infants Congenital Red Cell Aplastic Anaemia Congenital Pure Red Cell Anaemia Congenital Erythroid Hypoplasia Pearson Marrow-Pancreas Syndrome

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (9)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-174346	Skp2-IN-4		98.60%	Unkown
HY-12645	NCL1	Inhibitor	/	Unkown
HY-178048	Neuroprotective agent 13	Activator	/	Unkown
HY-178052	Neuroprotective agent 14	Activator	/	Unkown
HY-RS03886	DNAJC5 Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS09086	NCL Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-P5551	TLN-58	Inhibitor	/	Unkown
HY-RS17936	Ncl Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS24407	Ncl Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Felis catus	NCL	VGNC	VGNC:68430
Canis familiaris	NCL	VGNC	VGNC:43657
Mus musculus	NCL	MGD	MGI:97286
Macaca mulatta	NCL	VGNC	VGNC:75144
Rattus norvegicus	NCL	RGD	RGD:3153
Others	NCL	NCBI