2. Academic Validation
  3. The little elongation complex functions at initiation and elongation phases of snRNA gene transcription

The little elongation complex functions at initiation and elongation phases of snRNA gene transcription

  • Mol Cell. 2013 Aug 22;51(4):493-505. doi: 10.1016/j.molcel.2013.07.003.
Deqing Hu 1,  Edwin R Smith,  Alexander S Garruss,  Nima Mohaghegh,  Joseph M Varberg,  Chengqi Lin,  Jessica Jackson,  Xin Gao,  Anita Saraf,  Laurence Florens,  Michael P Washburn,  Joel C Eissenberg,  Ali Shilatifard
Affiliations

Affiliation

  • 1 Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA.
PMID: 23932780 DOI: 10.1016/j.molcel.2013.07.003
Abstract

The small nuclear RNA (snRNA) genes have been widely used as a model system for understanding transcriptional regulation due to the unique aspects of their promoter structure, selectivity for either RNA polymerase (Pol) II or III, and because of their unique mechanism of termination that is tightly linked with the promoter. Recently, we identified the little elongation complex (LEC) in Drosophila that is required for the expression of Pol II-transcribed snRNA genes. Here, using Drosophila and mammalian systems, we provide genetic and molecular evidence that LEC functions in at least two phases of snRNA transcription: an initiation step requiring the ICE1 subunit, and an elongation step requiring ELL.

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