2. Academic Validation
  3. A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation

A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation

  • Nat Chem Biol. 2014 Feb;10(2):93-5. doi: 10.1038/nchembio.1432.
Jianzhao Liu 1,  Yanan Yue 1,  Dali Han 2,  Xiao Wang 2,  Ye Fu 2,  Liang Zhang 2,  Guifang Jia 2,  Miao Yu 2,  Zhike Lu 2,  Xin Deng 2,  Qing Dai 2,  Weizhong Chen 2,  Chuan He 2
Affiliations

Affiliations

  • 1 1] Department of Chemistry, University of Chicago, Chicago, Illinois, USA. [2] Institute for Biophysical Dynamics, University of Chicago, Chicago, Illinois, USA. [3].
  • 2 1] Department of Chemistry, University of Chicago, Chicago, Illinois, USA. [2] Institute for Biophysical Dynamics, University of Chicago, Chicago, Illinois, USA.
PMID: 24316715 DOI: 10.1038/nchembio.1432
Abstract

N(6)-methyladenosine (m(6)A) is the most prevalent and reversible internal modification in mammalian messenger and noncoding RNAs. We report here that human methyltransferase-like 14 (METTL14) catalyzes m(6)A RNA methylation. Together with METTL3, the only previously known m(6)A methyltransferase, these two proteins form a stable heterodimer core complex of METTL3-METTL14 that functions in cellular m(6)A deposition on mammalian nuclear RNAs. WTAP, a mammalian splicing factor, can interact with this complex and affect this methylation.

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