2. Academic Validation
  3. MicroRNA miR-93-5p regulates expression of IL-8 and VEGF in neuroblastoma SK-N-AS cells

MicroRNA miR-93-5p regulates expression of IL-8 and VEGF in neuroblastoma SK-N-AS cells

  • Oncol Rep. 2016 May;35(5):2866-72. doi: 10.3892/or.2016.4676.
Enrica Fabbri 1,  Giulia Montagner 1,  Nicoletta Bianchi 1,  Alessia Finotti 1,  Monica Borgatti 1,  Ilaria Lampronti 1,  Giulio Cabrini 2,  Roberto Gambari 1
Affiliations

Affiliations

  • 1 Department of Life Sciences and Biotechnology, I-44121 Ferrara, Italy.
  • 2 Department of Pathology and Diagnostics, University-Hospital, I-37126 Verona, Italy.
PMID: 26986724 DOI: 10.3892/or.2016.4676
Abstract

The role of the MicroRNA miR-93-5p on the secretome profile and the expression levels of vascular endothelial growth factor (VEGF) and interleukin-8 (IL-8) was investigated in the neuroblastoma SK-N-AS cell line by Bio-Plex analysis and RT-qPCR. The results indicate that VEGF and IL-8 are the major miR-93-5p molecular targets. This conclusion was based on in vitro transfection with pre-miR-93-5p and anti-miR-93-5p; these treatments inversely modulated both VEGF and IL-8 gene expression and protein release in the neuroblastoma SK-N-AS cell line. Computational analysis showed the presence of miR-93-5p consensus sequences in the 3'UTR region of both VEGF and IL-8 mRNAs, predicting possible interaction with miR-93-5p and confirming a potential regulatory role of this MicroRNA.

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