2. Academic Validation
  3. Cep44 functions in centrosome cohesion by stabilizing rootletin

Cep44 functions in centrosome cohesion by stabilizing rootletin

  • J Cell Sci. 2020 Feb 21;133(4):jcs239616. doi: 10.1242/jcs.239616.
Delowar Hossain 1 2,  Sunny Y-P Shih 1,  Xintong Xiao 1,  Julia White 1,  William Y Tsang 3 2 4
Affiliations

Affiliations

  • 1 Institut de Recherches Cliniques de Montréal, 110 avenue des Pins Ouest, Montréal, Québec H2W 1R7, Canada.
  • 2 Division of Experimental Medicine, McGill University, Montréal, Québec H3A 1A3, Canada.
  • 3 Institut de Recherches Cliniques de Montréal, 110 avenue des Pins Ouest, Montréal, Québec H2W 1R7, Canada [email protected].
  • 4 Faculté de Médecine, Département de pathologie et Biologie Cellulaire, Université de Montréal, Montréal, Québec H3C 3J7, Canada.
PMID: 31974111 DOI: 10.1242/jcs.239616
Abstract

The centrosome linker serves to hold the duplicated centrosomes together until they separate in late G2/early mitosis. Precisely how the linker is assembled remains an open question. In this study, we identify Cep44 as a novel component of the linker in human cells. Cep44 localizes to the proximal end of centrioles, including mother and daughter centrioles, and its ablation leads to loss of centrosome cohesion. Cep44 does not impinge on the stability of C-Nap1 (also known as CEP250), LRRC45 or Cep215 (also known as CDK5RAP2), and vice versa, and these proteins are independently recruited to the centrosome. Rather, Cep44 associates with rootletin and regulates its stability and localization to the centrosome. Our findings reveal a role of the previously uncharacterized protein Cep44 for centrosome cohesion and linker assembly.

Keywords

Centrosome; Cep44; Cohesion; Rootletin; Splitting.

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