N-Desmethyldauricine
N-Desmethyldauricine is a NF-κB p65 inhibitor and apoptosis inducer. N-Desmethyldauricine reduces the protein expression level of p65, induces apoptosis, arrests the cell cycle at the G0/G1 phase, attenuates intercellular adhesion, and inhibits the growth of 3D spheroids of triple-negative breast cancer. N-Desmethyldauricine can be used in studies related to triple-negative breast cancer.
For research use only. We do not sell to patients.
- CAS No.: 146763-55-5
- Formula: C37H42N2O6
- Molecular Weight:610.74
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
N-Desmethyldauricine (72 h) potently inhibits the proliferation of human triple-negative breast cancer cell lines SUM159, HCC1937, MDA-MB-231, and MDA-MB-468, with IC50 values ranging from 5.73 μM to 13.16 μM after 72 h of treatment[1].
N-Desmethyldauricine (10-30 μM; 24 h) induces apoptosis in human triple-negative breast cancer SUM159 cells, increasing apoptotic cell percentage to 22.77% at 30 μM after 24 h, and upregulates the proapoptotic protein cleaved-poly-ADP-ribose polymerase 1 in a concentration-dependent manner[1].
N-Desmethyldauricine (10-30 μM; 24 h) arrests the cell cycle of human triple-negative breast cancer SUM159 cells in the G0/G1 phase, with 79.8% of cells in G0/G1 at 30 μM after 24 h, and downregulates the G0/G1 phase-related proteins cyclin-dependent kinase 2 and cyclin D1 in a concentration-dependent manner[1].
N-Desmethyldauricine (10-30 μM; 24 h) suppresses the migration of human triple-negative breast cancer SUM159 cells in a concentration-dependent manner, and downregulates the migration-related protein matrix metallopeptidase 9 in a concentration-dependent manner[1].
N-Desmethyldauricine (10-60 μM) downregulates the expression of p65, an important subunit of the NF-κB pathway, in human triple-negative breast cancer cells in vitro in a concentration-dependent manner[1].
N-Desmethyldauricine (25-100 μM; 10 days) effectively suppresses the growth of human triple-negative breast cancer SUM159 3D tumor spheroids, with 83% growth inhibition at 100 μM after 10 days of treatment, and reduces cell-cell adhesion in spheroids[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:SUM159, HCC1937, MDA-MB-231, MDA-MB-468
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Concentration:Titrated for IC50 determination
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Incubation Time:72 h
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Result:Inhibited proliferation of all four cell lines in a concentration-dependent manner, with IC50 values of 5.93 μM (SUM159), 13.16 μM (HCC1937), 7.55 μM (MDA-MB-231), and 5.73 μM (MDA-MB-468).
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Cell Line:SUM159
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Concentration:10-30 μM (Apoptosis assay); 10, 20, 40, 60 μM (Western Blot)
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Incubation Time:24 h (Apoptosis assay)
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Result:Increased the percentage of apoptotic SUM159 cells from 3.75% (untreated) to 5.16% (10 μM), 10.22% (20 μM), and 22.77% (30 μM).
Significantly increased the relative protein level of cleaved-poly-ADP-ribose polymerase 1 in a concentration-dependent manner.
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Cell Line:SUM159
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Concentration:10-30 μM (Flow cytometry); 10, 20, 40, 60 μM (Western Blot)
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Incubation Time:24 h (Flow cytometry)
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Result:Increased the percentage of SUM159 cells in the G0/G1 phase from 56.22% (untreated) to 67.14% (20 μM) and 79.8% (30 μM).
Significantly decreased the relative protein levels of G0/G1 phase-related proteins cyclin-dependent kinase 2 and cyclin D1 in a concentration-dependent manner.
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Cell Line:SUM159
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Concentration:10-30 μM (Wound healing); 10, 20, 40, 60 μM (Western Blot)
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Incubation Time:24 h (Wound healing)
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Result:Reduced the migration rate of SUM159 cells in a concentration-dependent manner after 24 h.
Significantly decreased the relative protein level of migration-related matrix metallopeptidase 9 in a concentration-dependent manner.
Chemical Information
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CAS No. 146763-55-5
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Molecular Weight 610.74
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Formula C37H42N2O6
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SMILES
COC(C=C(CCN1C)C([C@H]1CC2=CC=C(C=C2)OC3=CC(C[C@@H]4C5=CC(OC)=C(OC)C=C5CCN4)=CC=C3O)=C6)=C6OC
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
N-Desmethyldauricine Related Classifications
HY-N18190 Related Classifications
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