Ultrafast Detection of Peroxynitrite in Parkinson's Disease Models Using a Near-Infrared Fluorescent Probe

  • Anal Chem. 2020 Mar 3;92(5):4038-4045. doi: 10.1021/acs.analchem.9b05599.
Qian Sun  1  2 ,  Jiajia Xu  1 ,  Chonglei Ji  3 ,  Mazen S S Shaibani  2 ,  Zheng Li  1 ,  Kahleong Lim  4 ,  Chengwu Zhang  1 ,  Lin Li  1 ,  Zhipeng Liu  1  2
Affiliations
  • 1. Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing 211816, P. R. China.
  • 2. College of Materials Science and Engineering, Nanjing Forestry University, 159 Longpan Road, Xuanwu District, Nanjing 210037, P. R. China.
  • 3. Department of Chemistry, Zhejiang University, Hangzhou 310027, P. R. China.
  • 4. Department of Physiology, National University of Singapore, 2 Medical Drive, Singapore 117593.
PMID: 32028762 DOI: 10.1021/acs.analchem.9b05599
Abstract

Parkinson's disease (PD) is a neurodegenerative disease that devastatingly affects people's lives. Numerous research studies have shown that peroxynitrite (ONOO-) plays a pivotal role in the pathogenesis of PD. However, a suitable tool that could quickly and sensitively detect ONOO- in various PD models is still lacking. To this end, we designed and synthesized a series of near-infrared probes that could detect ONOO- within seconds by near-infrared fluorescent imaging in an ultrafast and highly selective manner. It is noteworthy that one of those developed probes, NIR-PN1, showed excellent sensing performance and blood-brain barrier penetrating ability. NIR-PN1 was successfully applied for imaging of ONOO- fluxes in multiple PD models including PC12 cell, Drosophila, C. elegans, and mouse brain, indicating its great potential application not only for understanding the biological roles that ONOO- played in PD but also for early PD diagnosis and treatment.

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