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Cat. No.: HY-E70094

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T4 UvsX Recombinase

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T4 UvsX Recombinase helps initiate DNA replication on a double-stranded DNA template by catalyzing synapsis between the template and a homologous DNA single strand that serves as primer. T4 UvsX Recombinase greatly amplifies the snap-back (hairpin-primed) DNA synthesis that is catalyzed by the T4 DNA polymerase holoenzyme on linear, single-stranded DNA templates.

For research use only. We do not sell to patients.

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

T4 UvsX Recombinase Chemical Structure

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

DNA Polymerase

All DNA/RNA Synthesis Isoforms :

In Vitro

Product Information
T4 UvsX recombinase is derived from T4 bacteriophage and is a homolog of the RecA/Rad51 family. It plays an important role in the repair of double-strand DNA breaks and the restart of replication forks. It is recombinantly expressed in E.coli and has no nuclease activity. The storage solution is 50 mM Tris-HCl, 50 mM KCI, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, pH 7.5.

Instructions
Applied to isothermal amplification RPA reactions, and the recommended addition amount is 300-400 ng/μL.

Precautions
Avoid repeated freezing and thawing.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

Appearance Liquid
Color Colorless to light yellow
SMILES

[T4 UvsX Recombinase]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (265 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Morrical SW, et al. Amplification of snap-back DNA synthesis reactions by the uvsX recombinase of bacteriophage T4. J Biol Chem. 1991 Jul 25;266(21):14031-8. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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