1. Anti-infection
  2. Arenavirus
  3. GP(33-41)

GP(33-41) 

Cat. No.: HY-P0323
Handling Instructions

GP(33-41), a 9-aa-long peptide, is the optimal sequence of the GP1 epitope of lymphocytic choriomeningitis virus, and can upregulate H-2Db molecules at the RMA-S (Db Kb) cell surface with SC50 of 344 nM.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

GP(33-41) Chemical Structure

GP(33-41) Chemical Structure

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Description

GP(33-41), a 9-aa-long peptide, is the optimal sequence of the GP1 epitope of lymphocytic choriomeningitis virus, and can upregulate H-2Db molecules at the RMA-S (Db Kb) cell surface with SC50 of 344 nM.

In Vitro

GP(33-41) sensitizes MC57 and T2-Db cells to lysis with ED50s of 0.9 ± 0.6 and 2.5 ± 0.7 nM[1].

Molecular Weight

1016.18

Formula

C₄₆H₆₉N₁₁O₁₃S

Sequence

Lys-Ala-Val-Tyr-Asn-Phe-Ala-Thr-Cys

Sequence Shortening

KAVYNFATC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
Kinase Assay
[1]

Binding experiments are performed at 37°C with T2-Db cells, with a Millipore MultiScreen assay system. The H-2Db LCMV antigen gp276-286 (SGVENPGGYCL) is radioiodinated, and the radiolabeled peptide is purified. Cells (2×105 per well) are incubated in MultiScreen-HV 96-well filtration plates (pore size, 0.45 mm) with 125I-gp276-286 (10 nM [final concentration]) for 90 min at 37°C. Cells are washed three times with ice-cold 1% BSA-PBS and by filtration under vacuum. The radioactivity bound to the cells retained on the filter is counted with a gamma counter. Direct binding is measured in the absence (total binding) or the presence (nonspecific binding) of a 1,000-fold excess (10 mM) of unlabeled gp276-286. Specific binding to H-2Db is defined as the difference between total binding and nonspecific binding. Nontransfected T2 cells are used as a negative control under the same experimental conditions. Competition assays are performed with increasing concentrations (10-10 to 10-5 M) of unlabeled peptides competing against a fixed concentration (10-8 M) of 125Igp276-286. The percent inhibition of binding is calculated as 100 × [1-(counts per minute in the presence of competitor - counts per minute of nonspecific binding)/counts per minute of specific binding].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Keywords:

GP(33-41)ArenavirusInhibitorinhibitorinhibit

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