1. Anti-infection
  2. Arenavirus
  3. GP(33-41)

GP(33-41), a 9-aa-long peptide, is the optimal sequence of the GP1 epitope of lymphocytic choriomeningitis virus, and can upregulate H-2Db molecules at the RMA-S (Db Kb) cell surface with a SC50 of 344 nM.

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GP(33-41) Chemical Structure

GP(33-41) Chemical Structure

CAS No. : 161928-86-5

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Description

GP(33-41), a 9-aa-long peptide, is the optimal sequence of the GP1 epitope of lymphocytic choriomeningitis virus, and can upregulate H-2Db molecules at the RMA-S (Db Kb) cell surface with a SC50 of 344 nM[1].

In Vitro

GP(33-41) sensitizes MC57 and T2-Db cells to lysis with ED50s of 0.9±0.6 and 2.5±0.7 nM[1].
The interaction between T cell receptors (TCR) and peptide-major histocompatibility complex (pMHC) antigens can lead to varying degrees of agonism (T cell activation), or antagonism. The P14 TCR recognises the lymphocytic choriomeningitis virus (LCMV)-derived peptide, GP(33-41) (KAVYNFATC), presents in the context of H-2D[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1016.17

Formula

C46H69N11O13S

CAS No.
Sequence

Lys-Ala-Val-Tyr-Asn-Phe-Ala-Thr-Cys

Sequence Shortening

KAVYNFATC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
Kinase Assay
[1]

Binding experiments are performed at 37°C with T2-Db cells, with a Millipore MultiScreen assay system. The H-2Db LCMV antigen gp276-286 (SGVENPGGYCL) is radioiodinated, and the radiolabeled peptide is purified. Cells (2×105 per well) are incubated in MultiScreen-HV 96-well filtration plates (pore size, 0.45 mm) with 125I-gp276-286 (10 nM [final concentration]) for 90 min at 37°C. Cells are washed three times with ice-cold 1% BSA-PBS and by filtration under vacuum. The radioactivity bound to the cells retained on the filter is counted with a gamma counter. Direct binding is measured in the absence (total binding) or the presence (nonspecific binding) of a 1,000-fold excess (10 mM) of unlabeled gp276-286. Specific binding to H-2Db is defined as the difference between total binding and nonspecific binding. Nontransfected T2 cells are used as a negative control under the same experimental conditions. Competition assays are performed with increasing concentrations (10-10 to 10-5 M) of unlabeled peptides competing against a fixed concentration (10-8 M) of 125Igp276-286. The percent inhibition of binding is calculated as 100 × [1-(counts per minute in the presence of competitor - counts per minute of nonspecific binding)/counts per minute of specific binding].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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GP(33-41) Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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