alpha-1,4-Galactosyltransferase (LgtC)
Based on 1 Customer Validation
alpha-1,4-Galactosyltransferase (LgtC) (A4GALT) is a glycosphingolipid-specific glycosyltransferase. alpha-1,4-Galactosyltransferase (LgtC) transfers a galactose to the alpha-1,4 position of lactosylceramide to form globotriaosylceramide. alpha-1,4-Galactosyltransferase (LgtC) can be used for the synthesis of P1 blood group antigens.
For research use only. We do not sell to patients.
- CAS No.: 52725-57-2
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Product Information This product is recombinantly expressed in E. coli, with an N-terminal His-tag, a molecular weight of 32 kDa, and a buffer composition of 20 mM Tris-HCl, pH 7.5, 5% glycerol. It can be used to transfer galactose to sugar chains with Gal as the non-reducing end. Instructions 1. Add substrate according to the reaction system, 5 mM MgCl2, 1 mM DTT, and appropriately add buffer salt (such as Tris-HCl), adjust the pH to 7.5, and add appropriate amount of LgtC (according to the reaction requirements); 2. After mixing, incubate the sample at 37 °C for 2-4h. Notes When the system is scaled up, the incubation time or enzyme amount needs to be adjusted according to the actual situation, and can be mixed by low-speed shaking.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
2.4.1.38
≥1 U/mg soild
One unit is defined as the amount of enzyme that will required to convert 1 μmol Gal from UDP-Gal to lactose forming Galα1,4 lac per minute at 37°C.
Technical Parameters
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Source
E. coli
Chemical Information
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CAS No. 52725-57-2
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[alpha-1,4-Galactosyltransferase (LgtC)]
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Synonyms
A4GALT
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (265 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Kaczmarek R, et al. Evaluation of an amino acid residue critical for the specificity and activity of human Gb3/CD77 synthase. Glycoconj J. 2016 Dec;33(6):963-973. [Content Brief]
[2]. Iwamura K, et al. The blood group P1 synthase gene is identical to the Gb3/CD77 synthase gene. A clue to the solution of the P1/P2/p puzzle. J Biol Chem. 2003 Nov 7;278(45):44429-38. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
alpha-1,4-Galactosyltransferase (LgtC) Related Classifications
HY-E70047 Related Classifications
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