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Cat. No.: HY-W1061585

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DNP Phosphoramidite

DNP Phosphoramidite is a non-nucleoside-based phosphoramidite carrying a 2,4-dinitrophenyl (DNP) hapten group, which enables multiple labeling of oligonucleotides during solid-phase synthesis. DNP Phosphoramidite binds to anti-DNP antibodies, allowing the detection of labeled oligonucleotides via enzyme-linked colorimetric techniques.

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CAS No.: 136949-85-4
Formule: C₂₁H₃₄N₅O₆P
Masse moléculaire:483.50

Stockage:

Please store the product under the recommended conditions in the Certificate of Analysis.

DNP Phosphoramidite Chemical Structure

CAS No.: 136949-85-4

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Activité biologique
- Description
- In Vitro
Chemical Information
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
Bioz

Activité biologique

Description

In Vitro

DNP Phosphoramidite (0.15 M; 5 h deprotection, 30 mins detritylation) enables efficient solid-phase labelling of oligonucleotides, with "trityl-on" synthesis preventing degradation during 5-hour 60°C concentrated ammonia deprotection, and resulting labelled oligonucleotides showing increased lipophilicity for easy HPLC purification^[1].
DNP Phosphoramidite enables DNP-labelled 24-mer oligonucleotides to exhibit optimal colorimetric dot blot detection sensitivity with 3 DNP labels, achieving limits comparable to biotin-labelled oligonucleotides regardless of spacer arm length^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 136949-85-4
Masse moléculaire 483.50
Formule C₂₁H₃₄N₅O₆P
SMILES

CC(N(P(OCCC#N)OCCCCCCNC(C([N+]([O-])=O)=C1)=CC=C1[N+]([O-])=O)C(C)C)C
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureté et documentation

Instruction de manipulation (2659 KB)

Références

[1]. Grzybowski J, et al. Synthesis and antibody-mediated detection of oligonucleotides containing multiple 2,4-dinitrophenyl reporter groups. Nucleic acids research. 1993 Apr 25;21(8):1705-12. [Content Brief]

Calculators

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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