4-Aminobenzamidine dihydrochloride
Based on 1 publication(s) in Google Scholar
4-Aminobenzamidine (p-Aminobenzamidine) dihydrochloride is a strong trypsin inhibitor. 4-Aminobenzamidine dihydrochloride acts as an orally active urokinase type plasminogen activator (uPA) inhibitor (Ki=82 μM). 4-Aminobenzamidine dihydrochloride can be used for cardiovascular diseases and anti-tumor study.
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- Reinheit: 99.97%
- CAS. Nr.: 2498-50-2
- Formel: C7H11Cl2N3
- Molecular Weight:208.09
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Speicherung:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) 4-Aminobenzamidine dihydrochloride
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Biologische Aktivität
4-Aminobenzamidine dihydrochloride causes a dose-dependent inhibition of uPA activity in DU145 cells[2].
4-Aminobenzamidine dihydrochloride has an anti-proliferative effect in DU145 cells. 4-Aminobenzamidine (100-250 μM, 24 h) abolishes rat hepatocyte proliferation induced by plasminogen and EGF[2][3].
4-Aminobenzamidine (1-100 μM) dihydrochloride induces a dose-dependent vasodilator effect in intact endothelium aortic rings from Wistar and SHR[4].
4-Aminobenzamidine (1 nM) dihydrochloride evokes coronary vasodilation in the isolated hearts of Wistar rats and SHR[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
4-Aminobenzamidine (0.01-10 μg/kg; i.v.; 5-min interval between each dose injection. 1mg/kg; p.o.; daily for 60 days) dihydrochloride prevents the progress of hypertension, improved endothelial function, and attenuated the cardiac remodeling in hypertensive rats[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Normotensive (Wistar) and spontaneously hypertensive (SHR) male rats (180-220 g and 11-12 weeks old)[4]
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Dosage:1 mg/kg
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Administration:Oral gavage (p.o.); daily for 60 days
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Result:Restored the endothelium-dependent vasorelaxation in the aorta from hypertensive rats.
Ameliorated sodium nitroprusside (SNP)-induced impaired relaxation in aortic rings from SHR.
Significantly attenuated the increase of systolic blood pressure (SBP) and improved the aorta vasorelaxation induced by acetylcholine and bradykinin-induced coronary vasodilation.
Reduced the cardiomyocyte diameter in SHR heart.
Significantly reduced the interstitial collagen content in the left ventricular tissue of SHR and attenuated the perivascular fibrosis area.
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Animal Model:Spontaneously hypertensive (SHR) male rats (16-20 weeks old)[4]
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Dosage:0.01, 0.1, 1, and 10 μg/kg (in 0.1 mL)
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Administration:Intravenous injection (i.v.); 5-min interval between each dose injection
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Result:Reduced mean arterial pressure (MAP) and increased both aortic (AVC) and mesenteric vascular conductance (MVC) in SHR.
Chemical Information
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CAS. Nr. 2498-50-2
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Appearance Solid
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Molecular Weight 208.09
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Formel C7H11Cl2N3
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Color White to off-white
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SMILES
N=C(N)C1=CC=C(N)C=C1.[H]Cl.[H]Cl
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Synonyms
p-Aminobenzamidine dihydrochloride
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
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Bioorg Chem
2022 Nov:128:106109. PMID: 36049322
Lösungsmittel & Löslichkeit
DMSO : 23.33 mg/mL (112.11 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (4.81 mM); Clear solution
This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 1 mg/mL (4.81 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 1 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Nguyen AL, et al. The development and application of a new affinity partitioning system for enzyme isolation and purification. Enzyme Microb Technol. 1990 Sep;12(9):663-8. [Content Brief]
[2]. Billström A, et al. The urokinase inhibitor p-aminobenzamidine inhibits growth of a human prostate tumor in SCID mice. Int J Cancer. 1995 May 16;61(4):542-7. [Content Brief]
[3]. Okumura N, et al. Cell surface-bound plasminogen regulates hepatocyte proliferation through a uPA-dependent mechanism. Biosci Biotechnol Biochem. 2007 Jun;71(6):1542-9. [Content Brief]
[4]. Dutra JBR, et al. p-Aminobenzamidine attenuates cardiovascular dysfunctions in spontaneously hypertensive rats. Life Sci. 2022 Sep 1;304:120693. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.8056 mL | 24.0278 mL | 48.0557 mL | 120.1392 mL |
| 5 mM | 0.9611 mL | 4.8056 mL | 9.6111 mL | 24.0278 mL | |
| 10 mM | 0.4806 mL | 2.4028 mL | 4.8056 mL | 12.0139 mL | |
| 15 mM | 0.3204 mL | 1.6019 mL | 3.2037 mL | 8.0093 mL | |
| 20 mM | 0.2403 mL | 1.2014 mL | 2.4028 mL | 6.0070 mL | |
| 25 mM | 0.1922 mL | 0.9611 mL | 1.9222 mL | 4.8056 mL | |
| 30 mM | 0.1602 mL | 0.8009 mL | 1.6019 mL | 4.0046 mL | |
| 40 mM | 0.1201 mL | 0.6007 mL | 1.2014 mL | 3.0035 mL | |
| 50 mM | 0.0961 mL | 0.4806 mL | 0.9611 mL | 2.4028 mL | |
| 60 mM | 0.0801 mL | 0.4005 mL | 0.8009 mL | 2.0023 mL | |
| 80 mM | 0.0601 mL | 0.3003 mL | 0.6007 mL | 1.5017 mL | |
| 100 mM | 0.0481 mL | 0.2403 mL | 0.4806 mL | 1.2014 mL |
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