PNU-176798
PNU-176798 is an antimicrobial agent, targeting protein synthesis in a wide spectrum of gram-positive and anaerobic bacteria.
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- CAS. Nr.: 428861-91-0
- Formel: C16H13FN4O3S
- Molecular Weight:360.36
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
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Chemical Information
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Biologische Aktivität
Bacterial[1]
PNU-176798 is an antimicrobial agent, with a minimum inhibitory concentration (MIC) of 1.4 μM for E. coli. PNU-176798 inhibits fMet-tRNA binding to the 70S ribosomes, with an IC50 of 32 μM. PNU-176798 also blocks translation, 70S initiation with IC50s of 0.53, and 32 μM, respectively. PNU-176798 inhibits peptidyl transferase (IC50, 40 μM), and the inhibition is more pronounced in the presence of elongation factor P (EF-P). PNU-176798 markedly inhibits the EF-G-mediated translocation of fMet-tRNA (IC50, 8 μM)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 428861-91-0
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Molecular Weight 360.36
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Formel C16H13FN4O3S
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SMILES
CC(NC[C@H]1CN(C2=CC=C(C3=NC=C(C#N)S3)C(F)=C2)C(O1)=O)=O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Protokoll
Binding of the f[35S]Met-tRNA to E. coli 70S ribosomes is conducted and the initiation reaction mixtures are prepared without initiation factors and contain 6 mM magnesium acetate [Mg(Ac)2], 0.08 μM AUG, 30 mM NH4Cl, 10 mM Tris (pH 7.4), and 20 pmol of 70S ribosomes in a final volume of 60 μL. The reaction mixtures are incubated for 15 min at 35°C, and the reactions are terminated by addition of cold buffer A, washed with buffer A, and counted by liquid scintillation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
PNU-176798 Related Classifications
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