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Art. -Nr.: HY-W127795

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Fluo-3 ammonium

Fluo-3 ammonium is a cell-impermeable calcium ion (Ca²⁺) indicator. Fluo-3 ammonium itself is not fluorescent, but it becomes fluorescent after binding to calcium ions (Ex/Em = 488/525 nm).

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CAS. Nr.: 134907-84-9
Formel: C₃₅H₂₈Cl₂N₂O₁₃.xH₃N

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (272 KB)

Fluo-3 ammonium Chemical Structure

CAS. Nr.: 134907-84-9

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Biologische Aktivität
- Beschreibung
- In Vitro
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Fluo-3 ammonium is a cell-impermeable calcium ion (Ca²⁺) indicator. Fluo-3 ammonium itself is not fluorescent, but it becomes fluorescent after binding to calcium ions (Ex/Em = 488/525 nm)^[1].

In Vitro

Fluo-3 can be introduced into cells using methods such as patch clamping and microinjection.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 134907-84-9
Formel C₃₅H₂₈Cl₂N₂O₁₃.xH₃N
SMILES

O=C(CN(CC(O)=O)C1=C(OCCOC2=C(N(CC(O)=O)CC(O)=O)C=CC(C3=C4C=C(C(C=C4OC5=C3C=C(Cl)C(O)=C5)=O)Cl)=C2)C=CC=C1)O.N.[x]
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. P A Vandenberghe, et al. Flow cytometric measurement of cytoplasmic free calcium in human peripheral blood T lymphocytes with fluo-3, a new fluorescent calcium indicator. J Immunol Methods. 1990 Mar 9;127(2):197-205. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

Minimum order quantity

100 mg

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