Alkaloids from the mangrove endophytic fungus Diaporthe phaseolorum SKS019

  • Bioorg Med Chem Lett. 2017 Feb 15;27(4):803-807. doi: 10.1016/j.bmcl.2017.01.029.
Hui Cui  1 ,  Jianchen Yu  2 ,  Senhua Chen  1 ,  Meng Ding  1 ,  Xishan Huang  1 ,  Jie Yuan  2 ,  Zhigang She  3
Affiliations
  • 1. School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China.
  • 2. Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
  • 3. School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou 510006, China. Electronic address: [email protected].
PMID: 28119026 DOI: 10.1016/j.bmcl.2017.01.029
Abstract

Six new Alkaloids including four new chromeno[3,2-c]pyridines, diaporphasines A-D (1-4), and two new isoindolinones, meyeroguillines C and D (6-7), as well as three known compounds meyeroguilline A (5), 5-deoxybostrycoidin (8), and fusaristatin A (9), were isolated from an endophytic fungus Diaporthe phaseolorum SKS019. Their structures were determined by analysis of 1D and 2D NMR and mass spectroscopic data. Compounds 1-9 are alkaloid components reported for the first time from the Diaporthe sp., and diaporphasines A-D (1-4) are the third examples of Alkaloids possessing the unique chromeno[3,2-c]pyridine nucleus. All isolated compounds 1-9 were evaluated for their cytotoxic activity in vitro using MDA-MB-435, HepG2, MCF10A, HCT116, and NCI-H460 human cell lines. Compound 8 exhibited cytotoxicity against MDA-MB-435 and NCI-H460 human Cancer cell lines with IC50 values of 5.32 and 6.57μM, respectively, and compound 9 showed growth-inhibitory activity against MDA-MB-435 human Cancer cell line with IC50 value of 8.15μM.

Keywords
Alkaloid; Chromeno[3,2-c]pyridine; Cytotoxicity; Diaporthe phaseolorum; Isoindolinone.