Anemarrhenasaponin Ia
Anemarrhenasaponin Ia is a steroidal saponin that can be isolated from the rhizomes of Anemarrhena asphodeloides Bunge. Anemarrhenasaponin Ia inhibits platelet aggregation. Anemarrhenasaponin Ia induces mild concentration-dependent hemolysis. Anemarrhenasaponin Ia inhibits fMLP- and AA-induced superoxide anion production, while enhancing PMA-induced superoxide anion production. Anemarrhenasaponin Ia can be used in studies related to thrombosis.
For research use only. We do not sell to patients.
- Purity: 99.94%
- CAS No.: 221317-02-8
- Formula: C40H68O14
- Molecular Weight:772.96
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Anemarrhenasaponin Ia (0.05 mM) significantly inhibits platelet aggregation, as evidenced by a 1.33 s delay in the activated partial thromboplastin time (APTT) of citrated plasma[1].
Anemarrhenasaponin Ia (0.2-0.5 mM; 5 min) induces mild, concentration-dependent hemolysis in red blood cells[1].
Anemarrhenasaponin Ia (0-100 μM; 3 min) concentration-dependently inhibits fMLP- and AA-induced superoxide anion production in neutrophils, while enhances PMA-induced superoxide anion production; specifically, treatment with 40 μM reduces fMLP-induced production to 23.3% of that in the control group, increases PMA-induced production to 299% of that in the control group, and reduces AA-induced production to 48.7% of that in the control group[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 221317-02-8
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Appearance Solid
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Molecular Weight 772.96
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Formula C40H68O14
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Color Off-white to light yellow
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SMILES
C[C@@]12[C@]3([H])[C@](OC(OC)([C@H]3C)CCC(C)C)([H])[C@@H]([C@@]1([H])[C@@]4([H])[C@]([C@@]5([C@](C[C@H](CC5)O[C@H]6[C@@H]([C@H]([C@H]([C@H](O6)CO)O)O)O[C@@H]7O[C@@H]([C@H]([C@@H]([C@H]7O)O)O)CO)([H])CC4)C)([H])CC2)O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 50 mg/mL (64.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (1.62 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Zhang J, et al. Effect of six steroidal saponins isolated from anemarrhenae rhizoma on platelet aggregation and hemolysis in human blood. Clin Chim Acta. 1999;289(1-2):79-88. [Content Brief]
[2]. Zhang J, et al. Effect of steroidal saponins of Anemarrhenae rhizoma on superoxide generation in human neutrophils. Biochem Biophys Res Commun. 1999;259(3):636-639. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2937 mL | 6.4686 mL | 12.9373 mL | 32.3432 mL |
| 5 mM | 0.2587 mL | 1.2937 mL | 2.5875 mL | 6.4686 mL | |
| 10 mM | 0.1294 mL | 0.6469 mL | 1.2937 mL | 3.2343 mL | |
| 15 mM | 0.0862 mL | 0.4312 mL | 0.8625 mL | 2.1562 mL | |
| 20 mM | 0.0647 mL | 0.3234 mL | 0.6469 mL | 1.6172 mL | |
| 25 mM | 0.0517 mL | 0.2587 mL | 0.5175 mL | 1.2937 mL | |
| 30 mM | 0.0431 mL | 0.2156 mL | 0.4312 mL | 1.0781 mL | |
| 40 mM | 0.0323 mL | 0.1617 mL | 0.3234 mL | 0.8086 mL | |
| 50 mM | 0.0259 mL | 0.1294 mL | 0.2587 mL | 0.6469 mL | |
| 60 mM | 0.0216 mL | 0.1078 mL | 0.2156 mL | 0.5391 mL |
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