2. Protein Tyrosine Kinase/RTK
  3. FAK
  4. FAK-IN-9

FAK-IN-9 (Compound 8f) is a potent and orally active FAK inhibitor with an IC50 of 27.44 nM. FAK-IN-9 induces triple-negative breast cancer (TNBC) cell apoptosis.

For research use only. We do not sell to patients.

FAK-IN-9 Chemical Structure

FAK-IN-9 Chemical Structure

CAS No. : 2911655-93-9

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  
Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

FAK-IN-9 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

FAK-IN-9 (Compound 8f) is a potent and orally active FAK inhibitor with an IC50 of 27.44 nM. FAK-IN-9 induces triple-negative breast cancer (TNBC) cell apoptosis[1].

IC50 & Target

IC50: 27.44 nM (FAK)[1]
In Vitro

FAK-IN-9 (Compound 8f; 72 h) shows antiproliferative activity with IC50s of 0.167±0.025, 0.126±0.012 and 0.159±0.017 μM against MDA-MB-157, MDA-MB-231 and MDA-MB-453 cells, respectively[1].
FAK-IN-9 (1-4 μM; 72 h) leads to relatively high levels of NO production in a dose-dependent manner in MDA-MB-231 cells[1].
FAK-IN-9 (1-4 μM; 48 h) inhibits invasion and migration of MDA-MB-231 cells[1].
FAK-IN-9 (1-4 μM; 72 h) efficiently blocks FAK mediated-signaling pathways[1].
FAK-IN-9 (4 μM; 72 h) inhibits the formation of focal adhesions (FAs) and stress fibers (SFs) in MDA-MB-231 cells[1].
FAK-IN-9 (1-4 μM; 72 h) induces MDA-MB-231 cell apoptosis[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

FAK-IN-9 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: MDA-MB-157, MDA-MB-231, MDA-MB-453 and MCF10A
Concentration:
Incubation Time: 72 h
Result: Inhibited proliferation with IC50s of 0.167±0.025, 0.126±0.012, 0.159±0.017 and 2.401±0.131 μM against MDA-MB-157, MDA-MB-231, MDA-MB-453 and MCF10A, respectively.

Cell Invasion Assay[1]

Cell Line: MDA-MB-231 cells
Concentration: 1, 2 and 4 μM
Incubation Time: 48 h
Result: The numbers of invasive MDA-MB-231 cells were reduced dose-dependently.

Cell Migration Assay [1]

Cell Line: MDA-MB-231 cells
Concentration: 1, 2 and 4 μM
Incubation Time: 48 h
Result: Remarkably block the migration of MDA-MB-231 cells in a dose-dependent manner.

Western Blot Analysis[1]

Cell Line: MDA-MB-231 cells
Concentration: 1, 2 and 4 μM
Incubation Time: 72 h
Result: Potently suppressed the autophosphorylation of Y397 in a dose-dependent manner. Decreased the levels of p-AKT, MMP-2 and MMP-9 dose dependently.

Apoptosis Analysis[1]

Cell Line: MDA-MB-231 cells
Concentration: 1, 2 and 4 μM
Incubation Time: 72 h
Result: The percentage of apoptotic MDA-MB-231 cells gradually increased ranging from 19.06% to 77.66% at 4 μM.
In Vivo

FAK-IN-9 (Compound 8f; 15 or 30 mg/kg; oral; once daily for 30 days) inhibits MDA-MB-231 lung metastasis in mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c nude mice, MDA-MB-231 experimental pulmonary metastasis model[1]
Dosage: 15 or 30 mg/kg
Administration: Oral, once daily for 30 days
Result: Potently reduced the numbers of lung tumor nodules dose-dependently.
Molecular Weight

764.25

Formula

C36H38ClN7O8S
CAS No.
SMILES

CNC(C1=C(C=CC=C1)NC2=C(C=NC(NC3=CC=C(C=C3)CC(OCCCCCCCCOC4=NO[N+]([O-])=C4S(=O)(C5=CC=CC=C5)=O)=O)=N2)Cl)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

FAK-IN-9 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

FAK-IN-92911655-93-9FAKPTK2 protein tyrosine kinase 2PTK2Focal adhesion kinasetriple-negative breast cancerTNBCMDA-MB-157MDA-MB-231MDA-MB-453Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
FAK-IN-9
Cat. No.:
HY-149259
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.