Biotin-PEG5-NHS ester
Based on 1 Customer Validation
Biotin-PEG5-NHS ester is a heterobifunctional crosslinker as well as a polyethylene glycol-containing bioconjugation reagent. Biotin-PEG5-NHS ester is widely used in scientific research fields such as the construction of highly sensitive biosensors, the development of targeted drug delivery systems (e.g., liposome modification), immunofluorescence imaging, and protein affinity purification, and serves as an important tool for achieving precise labeling and functionalization of biomolecules.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 98.0%
- 分子式: C27H44N4O11S
- 分子量:632.72
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保管条件:Pure form -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
生物活性
Biotin-PEG5-NHS ester is a highly efficient bioconjugation reagent, mainly used for the specific introduction of biotin tags onto molecules containing primary amine groups (-NH2) (such as proteins, antibodies, polypeptides) or material surfaces. The NHS ester group in its molecular structure rapidly reacts with amine groups under mild conditions to form stable amide bonds, while the intermediate PEG5 chain (pentaethylene glycol) acts as a flexible spacer arm to increase the spatial freedom of the molecule, and can also significantly improve water solubility and reduce non-specific adsorption.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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性状 Oil
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分子量 632.72
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分子式 C27H44N4O11S
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Color White to off-white
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SMILES
[H][C@]12NC(N[C@]1(CS[C@H]2CCCCC(NCCOCCOCCOCCOCCOCCC(ON3C(CCC3=O)=O)=O)=O)[H])=O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Pure form -20°C 3 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
DMSO : 100 mg/mL (158.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (266 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5805 mL | 7.9024 mL | 15.8048 mL | 39.5120 mL |
| 5 mM | 0.3161 mL | 1.5805 mL | 3.1610 mL | 7.9024 mL | |
| 10 mM | 0.1580 mL | 0.7902 mL | 1.5805 mL | 3.9512 mL | |
| 15 mM | 0.1054 mL | 0.5268 mL | 1.0537 mL | 2.6341 mL | |
| 20 mM | 0.0790 mL | 0.3951 mL | 0.7902 mL | 1.9756 mL | |
| 25 mM | 0.0632 mL | 0.3161 mL | 0.6322 mL | 1.5805 mL | |
| 30 mM | 0.0527 mL | 0.2634 mL | 0.5268 mL | 1.3171 mL | |
| 40 mM | 0.0395 mL | 0.1976 mL | 0.3951 mL | 0.9878 mL | |
| 50 mM | 0.0316 mL | 0.1580 mL | 0.3161 mL | 0.7902 mL | |
| 60 mM | 0.0263 mL | 0.1317 mL | 0.2634 mL | 0.6585 mL | |
| 80 mM | 0.0198 mL | 0.0988 mL | 0.1976 mL | 0.4939 mL | |
| 100 mM | 0.0158 mL | 0.0790 mL | 0.1580 mL | 0.3951 mL |