PE-Cy5.5
PE-Cy5.5 is a far-red emitting tandem dye designed based on the principle of fluorescence resonance energy transfer (FRET), and is widely used in multicolor flow cytometry. PE-Cy5.5 consists of phycoerythrin (PE) as the energy donor and the cyanine dye Cy5.5 as the energy acceptor. Upon excitation by blue or green laser light, PE absorbs energy and transfers it to Cy5.5 via FRET, ultimately resulting in Cy5.5 emitting characteristic far-red fluorescence (Ex/Em = 450-500 nm/698 nm).
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
生物活性
PE-Cy5.5 conjugates (0.25-5 μg/mL; 30 min at 4 °C) bind strongly and nonspecifically to mouse DEC205-positive CHO/mDEC205, KIT6/mDEC205, and HEK293/mDEC205 cells in proportion to surface mouse DEC205 expression, do not bind to human DEC205-positive cells, and exhibit stronger binding than other Cy5.5-based conjugates[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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SMILES
[PE-Cy5.5]
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)