PGD Protein, Human (His)

PGD Protein is crucial in cellular metabolism, catalyzing the oxidative decarboxylation of 6-phosphogluconate. This process yields ribulose 5-phosphate, releases CO(2), and simultaneously reduces NADP to NADPH, contributing to essential cellular redox balance. The enzyme's functionality underscores its significance in maintaining cellular homeostasis and participating in key metabolic pathways. PGD Protein, Human (His) is the recombinant human-derived PGD protein, expressed by E. coli , with N-His labeled tag.

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  • Species: Human
  • Source: E. coli
  • 保管条件:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • 生物活性
  • Technical Parameters
  • Product Properties
  • ドキュメント
  • Help & FAQs

生物活性

製品説明

PGD Protein is crucial in cellular metabolism, catalyzing the oxidative decarboxylation of 6-phosphogluconate. This process yields ribulose 5-phosphate, releases CO(2), and simultaneously reduces NADP to NADPH, contributing to essential cellular redox balance. The enzyme's functionality underscores its significance in maintaining cellular homeostasis and participating in key metabolic pathways. PGD Protein, Human (His) is the recombinant human-derived PGD protein, expressed by E. coli , with N-His labeled tag.

Background

PGD Protein plays a pivotal role in cellular metabolism by catalyzing the oxidative decarboxylation of 6-phosphogluconate, leading to the formation of ribulose 5-phosphate and the release of CO(2). This enzymatic process is coupled with the simultaneous reduction of NADP to NADPH, contributing to vital cellular redox balance. The functionality of PGD Protein in mediating these biochemical reactions underscores its significance in maintaining cellular homeostasis and participating in key metabolic pathways.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-His
  • アクセッション番号
  • 遺伝子ID
  • Molecular Construction
    • N-term
    • His
    • PGD (M1-A483)
      Accession # P52209-1
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • 別名

    PGD; Phosphogluconate Dehydrogenase (NADP(+)-Dependent, Decarboxylating); Phosphogluconate Dehydrogenase; 6PGD; 6-Phosphogluconate Dehydrogenase, Decarboxylating; PGDH

  • AA Sequence

    MAQADIALIGLAVMGQNLILNMNDHGFVVCAFNRTVSKVDDFLANEAKGTKVVGAQSLKEMVSKLKKPRRIILLVKAGQAVDDFIEKLVPLLDTGDIIIDGGNSEYRDTTRRCRDLKAKGILFVGSGVSGGEEGARYGPSLMPGGNKEAWPHIKTIFQGIAAKVGTGEPCCDWVGDEGAGHFVKMVHNGIEYGDMQLICEAYHLMKDVLGMAQDEMAQAFEDWNKTELDSFLIEITANILKFQDTDGKHLLPKIRDSAGQKGTGKWTAISALEYGVPVTLIGEAVFARCLSSLKDERIQASKKLKGPQKFQFDGDKKSFLEDIRKALYASKIISYAQGFMLLRQAATEFGWTLNYGGIALMWRGGCIIRSVFLGKIKDAFDRNPELQNLLLDDFFKSAVENCQDSWRRAVSTGVQAGIPMPCFTTALSFYDGYRHEMLPASLIQAQRDYFGAHTYELLAKPGQFIHTNWTGHGGTVSSSSYNA

  • Predicted Molecular Mass

    54.6 kDa

  • 分子量

    Approximately 46 kDa, based on SDS-PAGE under reducing conditions.

  • 純度

    ≥ 85%, as determined by reducing SDS-PAGE.

Product Properties

性状

Solution

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

輸送条件

Shipping with dry ice.

Calculators

再構成計算機

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
希釈計算機

濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)

濃度 (開始) 濃度 (開始)
×
体積 (開始) 体積 (開始)
=
濃度 (終了) 濃度 (終了)
×
体積 (終了) 体積 (終了)
比活性計算機
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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