CESTA Assay Service

Cellular Thermal Shift Assay (CETSA) is a technique for directly detecting drug-target interactions within cells under physiological conditions. By monitoring changes in the thermal stability of target proteins upon drug binding, CETSA provides a direct indication of drug binding capacity and specificity in living cells. It is widely used in drug target identification, protein-ligand interaction studies, and drug screening. CETSA offers several advantages including high sensitivity, no requirement for protein purification, and applicability to complex biological systems such as protein lysates or living cells, making it a crucial tool in modern drug discovery and development and protein functional research.

How CETSA Works

CETSA is based on the principle that drug binding can alter the thermal stability of target proteins. Proteins are structurally stable under physiological conditions, however, as temperature rises, they gradually denature, transforming from a soluble state to an insoluble precipitate. For certain proteins, the melting temperature (Tm) is defined as the temperature at which 50% of the protein denatures. The Tm value reflects the stability of the protein structure. When small molecules (such as drugs) bind to target proteins, they increase the thermal stability of the target proteins. As the temperature increases, unbound proteins denature first, while drug-bound proteins remain relatively stable. Using techniques such as western blot or mass spectrometry (MS), the extent of protein degradation at different temperatures can be detected, allowing for the inference of drug binding to the target protein.

Applications

• Drug target
  validation
• Off-target effect screening
• Compound
  screening
• Mechanistic
  studies
• Biomarker
  discovery

Key Features

• No protein
  purification required
• Flexible sample sources
  and wide application
• Applicable to living cells
• Diverse detection
  methods

Quote for Service

CETSA Assay Service requires evaluation to determine the appropriate scheme and pricing For additional information about service price and technical specifics, please send an email to [email protected] or contact the sales staff of MedChemExpress directly.