Panosialins, inhibitors of an alpha1,3-fucosyltransferase Fuc-TVII, suppress the expression of selectin ligands on U937 cells

  • Glycoconj J. 1998 Nov;15(11):1079-83. doi: 10.1023/a:1006953626578.
K Shinoda  1 ,  K Shitara ,  Y Yoshihara ,  A Kusano ,  Y Uosaki ,  S Ohta ,  N Hanai ,  I Takahashi
Affiliations
PMID: 10386892 DOI: 10.1023/a:1006953626578
Abstract

Panosialins A and B were isolated as inhibitors of an alpha1,3-fucosyltransferase, Fuc-TVII, which is a key enzyme in the biosynthesis of Selectin ligands, from culture broth of Streptomyces sp. Panosialins A and B inhibited the Fuc-TVII activity with IC50 values of 4.8 and 5.3 microg/ml, respectively. Panosialin A suppressed expression of Selectin ligands on U937 cells, and inhibited the cell adhesion to immobilized E-selectin-immunoglobulin. Panosialins are the first reported Fuc-TVII inhibitors which can suppress the biosynthesis of Selectin ligands and then inhibit selectin-mediated cell adhesion.