1. Academic Validation
  2. Sp100 is important for the stimulatory effect of homeodomain-interacting protein kinase-2 on p53-dependent gene expression

Sp100 is important for the stimulatory effect of homeodomain-interacting protein kinase-2 on p53-dependent gene expression

  • Oncogene. 2003 Nov 27;22(54):8731-7. doi: 10.1038/sj.onc.1207079.
Andreas Möller 1 Hüseyin Sirma Thomas G Hofmann Hannah Staege Ekaterina Gresko Katharina Schmid Lüdi Elisabeth Klimczak Wulf Dröge Hans Will M Lienhard Schmitz
Affiliations

Affiliation

  • 1 German Cancer Research Center, Division of Immunochemistry (G0200), Im Neuenheimer Feld 280, Heidelberg D-69120, Germany.
Abstract

HIPK2 shows overlapping localization with p53 in promyelocytic leukemia (PML) nuclear bodies (PML-NBs) and functionally interacts with p53 to increase gene expression. Here we demonstrate that HIPK2 and the PML-NB resident protein Sp100 synergize for the activation of p53-dependent gene expression. Sp100 and HIPK2 interact and partially colocalize in PML-NBs. The cooperation of HIPK2 and Sp100 for the induction of p21(Waf1) is completely dependent on the presence of p53 and the kinase function of HIPK2. Downregulation of Sp100 levels by expression of siRNA does not interfere with p53-mediated transcription, but obviates the enhancing effect of HIPK2. In summary, these experiments reveal a novel function for Sp100 as a coactivator for HIPK2-mediated p53 activation.

Figures