2. Academic Validation
  3. SNAT2 silencing prevents the osmotic induction of transport system A and hinders cell recovery from hypertonic stress

SNAT2 silencing prevents the osmotic induction of transport system A and hinders cell recovery from hypertonic stress

  • FEBS Lett. 2005 Jun 20;579(16):3376-80. doi: 10.1016/j.febslet.2005.05.002.
Elena Bevilacqua 1,  Ovidio Bussolati,  Valeria Dall'Asta,  Francesca Gaccioli,  Roberto Sala,  Gian C Gazzola,  Renata Franchi-Gazzola
Affiliations

Affiliation

  • 1 Sezione di Patologia Generale e Clinica, Dipartimento di Medicina, Sperimentale, Università degli Studi di Parma, Via Volturno 39, 43100 Parma, Italy.
PMID: 15922329 DOI: 10.1016/j.febslet.2005.05.002
Abstract

Under hypertonic conditions the induction of SLC38A2/SNAT2 leads to the stimulation of transport system A and to the increase in the cell content of Amino acids. In hypertonically stressed human fibroblasts transfection with two siRNAs for SNAT2 suppressed the increase in SNAT2 mRNA and the stimulation of system A transport activity. Under the same condition, the expansion of the intracellular amino acid pool was significantly lowered and cell volume recovery markedly delayed. It is concluded that the up-regulation of SNAT2 is essential for the rapid restoration of cell volume after hypertonic stress.

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