Enzymatic total synthesis of rabelomycin, an angucycline group antibiotic

A one-pot enzymatic total synthesis of angucycline Antibiotic rabelomycin was accomplished, starting from acetyl-CoA and malonyl-CoA, using a mixture of polyketide synthase (PKS) Enzymes of the gilvocarcin, ravidomycin, and jadomycin biosynthetic pathways. The in vitro results were compared to in vivo catalysis using analogous sets of Enzymes.