Regulation of cell death in human fetal and adult ovaries--role of Bok and Bcl-X(L)

Of eight million oocytes formed in fetal ovaries, only 400 are ovulated and the rest are degraded via Apoptosis. Studies in rodents suggest an important role for Bok and Bcl-X(L) in ovarian Apoptosis, but their expression patterns and roles in human ovaries are not well known. Protein expression of Bok and Bcl-X(L) as well as the death pathway effectors TNF and Caspase-3 were determined in an important collection of samples consisting of human fetal and adult ovaries. A penetrant expression of Bok, Bcl-X(L), TNF and full length and cleaved Caspase-3 were characterized in fetal ovaries, with specific patterns in oocytes and pre-granulosa/granulosa cells. Bok and Bcl-X(L) were detected also in adult ovaries. Lentiviral shRNA delivery demonstrated that loss of Bok markedly reduces vulnerability to Apoptosis and, conversely, loss of Bcl-X(L) increases Apoptosis in human granulosa tumour cell line. The results suggest important roles for Bok and Bcl-X(L) in human ovarian development, follicle maturation and Apoptosis.