2. Academic Validation
  3. HELB Is a Feedback Inhibitor of DNA End Resection

HELB Is a Feedback Inhibitor of DNA End Resection

  • Mol Cell. 2016 Feb 4;61(3):405-418. doi: 10.1016/j.molcel.2015.12.013.
Ján Tkáč 1,  Guotai Xu 2,  Hemanta Adhikary 3,  Jordan T F Young 1,  David Gallo 4,  Cristina Escribano-Díaz 5,  Jana Krietsch 3,  Alexandre Orthwein 5,  Meagan Munro 5,  Wendy Sol 2,  Abdallah Al-Hakim 5,  Zhen-Yuan Lin 5,  Jos Jonkers 6,  Piet Borst 2,  Grant W Brown 4,  Anne-Claude Gingras 1,  Sven Rottenberg 7,  Jean-Yves Masson 3,  Daniel Durocher 8
Affiliations

Affiliations

  • 1 Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, ON M5G 1X5, Canada; Department of Molecular Genetics, University of Toronto, ON M5S 1A8, Canada.
  • 2 Division of Molecular Oncology, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, the Netherlands.
  • 3 Genome Stability Laboratory, CHU de Québec Research Center, HDQ Pavilion, Oncology Axis, 9 McMahon, Québec City, QC G1R 2J6, Canada; Department of Molecular Biology, Medical Biochemistry and Pathology, Laval University, Québec City, QC G1V 0A6, Canada.
  • 4 Department of Biochemistry and Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, ON M5S 3E1, Canada.
  • 5 Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, ON M5G 1X5, Canada.
  • 6 Division of Molecular Pathology, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, the Netherlands.
  • 7 Division of Molecular Oncology, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, the Netherlands; Institute of Animal Pathology, Vetsuisse Faculty, University of Bern, Laenggasstrasse 122, 3012 Bern, Switzerland.
  • 8 Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, ON M5G 1X5, Canada; Department of Molecular Genetics, University of Toronto, ON M5S 1A8, Canada. Electronic address: [email protected].
PMID: 26774285 DOI: 10.1016/j.molcel.2015.12.013
Abstract

DNA double-strand break repair by homologous recombination is initiated by the formation of 3' single-stranded DNA (ssDNA) overhangs by a process termed end resection. Although much focus has been given to the decision to initiate resection, little is known of the mechanisms that regulate the ongoing formation of ssDNA tails. Here we report that DNA helicase B (HELB) underpins a feedback inhibition mechanism that curtails resection. HELB is recruited to ssDNA by interacting with RPA and uses its 5'-3' ssDNA translocase activity to inhibit EXO1 and BLM-DNA2, the nucleases catalyzing resection. HELB acts independently of 53BP1 and is exported from the nucleus as cells approach S phase, concomitant with the upregulation of resection. Consistent with its role as a resection antagonist, loss of HELB results in PARP Inhibitor resistance in BRCA1-deficient tumor cells. We conclude that mammalian DNA end resection triggers its own inhibition via the recruitment of HELB.

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