2. Academic Validation
  3. Dual Chromatin and Cytoskeletal Remodeling by SETD2

Dual Chromatin and Cytoskeletal Remodeling by SETD2

  • Cell. 2016 Aug 11;166(4):950-962. doi: 10.1016/j.cell.2016.07.005.
In Young Park 1,  Reid T Powell 1,  Durga Nand Tripathi 1,  Ruhee Dere 1,  Thai H Ho 2,  T Lynne Blasius 3,  Yun-Chen Chiang 4,  Ian J Davis 5,  Catherine C Fahey 4,  Kathryn E Hacker 4,  Kristen J Verhey 3,  Mark T Bedford 6,  Eric Jonasch 7,  W Kimryn Rathmell 8,  Cheryl Lyn Walker 9
Affiliations

Affiliations

  • 1 Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M Health Science Center, Houston, TX 77030, USA.
  • 2 Division of Hematology/Oncology, Mayo Clinic Arizona, Scottsdale, AZ 85259, USA.
  • 3 Department of Cell and Developmental Biology, Medical School, University of Michigan, Ann Arbor, MI 48109, USA.
  • 4 Department of Genetics and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA.
  • 5 Department of Genetics and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA; Department of Pediatrics, University of North Carolina, Chapel Hill, NC 27599, USA.
  • 6 Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, Smithville, TX 78957, USA.
  • 7 Department of Genitourinary Medical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
  • 8 Department of Genetics and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA; Division of Hematology/Oncology, Vanderbilt-Ingram Cancer Center, Vanderbilt University, Nashville, TN 37232, USA.
  • 9 Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M Health Science Center, Houston, TX 77030, USA. Electronic address: [email protected].
PMID: 27518565 DOI: 10.1016/j.cell.2016.07.005
Abstract

Posttranslational modifications (PTMs) of tubulin specify microtubules for specialized cellular functions and comprise what is termed a "tubulin code." PTMs of histones comprise an analogous "histone code," although the "readers, writers, and erasers" of the Cytoskeleton and epigenome have heretofore been distinct. We show that methylation is a PTM of dynamic microtubules and that the Histone Methyltransferase SET-domain-containing 2 (SETD2), which is responsible for H3 lysine 36 trimethylation (H3K36me3) of histones, also methylates α-tubulin at lysine 40, the same lysine that is marked by acetylation on microtubules. Methylation of microtubules occurs during mitosis and cytokinesis and can be ablated by SETD2 deletion, which causes mitotic spindle and cytokinesis defects, micronuclei, and polyploidy. These data now identify SETD2 as a dual-function methyltransferase for both chromatin and the Cytoskeleton and show a requirement for methylation in maintenance of genomic stability and the integrity of both the tubulin and histone codes.

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