2. Academic Validation
  3. A Dock-and-Lock Mechanism Clusters ADAM10 at Cell-Cell Junctions to Promote α-Toxin Cytotoxicity

A Dock-and-Lock Mechanism Clusters ADAM10 at Cell-Cell Junctions to Promote α-Toxin Cytotoxicity

  • Cell Rep. 2018 Nov 20;25(8):2132-2147.e7. doi: 10.1016/j.celrep.2018.10.088.
Jimit Shah 1,  Florian Rouaud 1,  Diego Guerrera 1,  Ekaterina Vasileva 1,  Lauren M Popov 2,  William L Kelley 3,  Eric Rubinstein 4,  Jan E Carette 2,  Manuel R Amieva 2,  Sandra Citi 5
Affiliations

Affiliations

  • 1 Department of Cell Biology, Faculty of Sciences, University of Geneva, 1211-4 Geneva, Switzerland; Institute for Genetics and Genomics of Geneva (iGE3), University of Geneva, 1211-4 Geneva, Switzerland.
  • 2 Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • 3 Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, 1211-4 Geneva, Switzerland.
  • 4 INSERM, Université Paris-Sud, UMRS_935, 94807 Villejuif Cedex, France.
  • 5 Department of Cell Biology, Faculty of Sciences, University of Geneva, 1211-4 Geneva, Switzerland; Institute for Genetics and Genomics of Geneva (iGE3), University of Geneva, 1211-4 Geneva, Switzerland. Electronic address: [email protected].
PMID: 30463011 DOI: 10.1016/j.celrep.2018.10.088
Abstract

We previously identified PLEKHA7 and other junctional proteins as host factors mediating death by S. aureus α-toxin, but the mechanism through which junctions promote toxicity was unclear. Using cell biological and biochemical methods, we now show that ADAM10 is docked to junctions by its transmembrane partner Tspan33, whose cytoplasmic C terminus binds to the WW domain of PLEKHA7 in the presence of PDZD11. ADAM10 is locked at junctions through binding of its cytoplasmic C terminus to afadin. Junctionally clustered ADAM10 supports the efficient formation of stable toxin pores. Instead, disruption of the PLEKHA7-PDZD11 complex inhibits ADAM10 and toxin junctional clustering. This promotes toxin pore removal from the cell surface through an actin- and macropinocytosis-dependent process, resulting in cell recovery from initial injury and survival. These results uncover a dock-and-lock molecular mechanism to target ADAM10 to junctions and provide a paradigm for how junctions regulate transmembrane receptors through their clustering.

Keywords

ADAM10; Afadin; PDZD11; PLEKHA7; Staphylococcus auereus α-toxin; Tetraspanin; cell junctions; cell-death; epithelium; macropinocytosis.

Figures