Chemical constituents from Kandelia candel with their inhibitory effects on pro-inflammatory cytokines production in LPS-stimulated bone marrow-derived dendritic cells (BMDCs)

  • Bioorg Med Chem Lett. 2015 Apr 1;25(7):1412-6. doi: 10.1016/j.bmcl.2015.02.048.
Le Duc Dat  1 ,  Nguyen Phuong Thao  1 ,  Bui Huu Tai  1 ,  Bui Thi Thuy Luyen  2 ,  Sohyun Kim  3 ,  Jung Eun Koo  3 ,  Young Sang Koh  3 ,  Nguyen The Cuong  4 ,  Nguyen Van Thanh  5 ,  Nguyen Xuan Cuong  5 ,  Nguyen Hoai Nam  5 ,  Phan Van Kiem  5 ,  Chau Van Minh  6 ,  Young Ho Kim  7
Affiliations
  • 1. Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghido, Caugiay, Hanoi, Vietnam; College of Pharmacy, Chungnam National University, Daejeon 305-764, South Korea.
  • 2. College of Pharmacy, Chungnam National University, Daejeon 305-764, South Korea.
  • 3. School of Medicine, Brain Korea 21 PLUS Program, and Institute of Medical Science, Jeju National University, Jeju 690-756, South Korea.
  • 4. Institute of Ecology and Biological Resources, VAST, 18 Hoang Quoc Viet, Nghido, Caugiay, Hanoi, Vietnam.
  • 5. Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghido, Caugiay, Hanoi, Vietnam.
  • 6. Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghido, Caugiay, Hanoi, Vietnam. Electronic address: [email protected].
  • 7. College of Pharmacy, Chungnam National University, Daejeon 305-764, South Korea. Electronic address: [email protected].
PMID: 25769817 DOI: 10.1016/j.bmcl.2015.02.048
Abstract

Chemical investigation of Kandelia candel resulted in the isolation of 19 compounds (1-19), including one new sesquiterpene glycoside, kandelside (1), three megastigman glycoside compounds (7-9), 16 known phenolic compounds (2-6 and 10-19). Structures of the isolated compounds were elucidated based on spectral data comparison with reported values. Isolated compounds were also evaluated for their inhibitory effects on the production of pro-inflammatory cytokines interleukin (IL)-12 p40, IL-6, and tumor necrosis factor α (TNF-α) in lipopolysaccharide (LPS)-stimulated bone marrow-derived dendritic cells. Among these compounds, compound 9 exhibited strong inhibitory activity against IL-6 production (IC50=0.07 ± 0.05 μM) and moderate inhibitory activity against TNF-α production (IC50=49.86 ± 1.02 μM), but exhibited no activity on IL-12 p40 production. Compounds 5 and 6 significantly inhibited IL-12 p40, IL-6, and TNF-α production with IC50 values of 11.68 ± 0.38, 44.52 ± 1.08, and 28.73 ± 0.96 μM, respectively.

Keywords
Anti-inflammatory activity; Kandelia candel; Kandelside; Rhizpphoraceae.