Interleukin-3 Prevents Cellular Death Induced by Oxidative Stress in HEK293 Cells
- J Cell Biochem. 2017 Jun;118(6):1330-1340. doi: 10.1002/jcb.25790.
- 1. Facultad de Ciencias, Instituto de Bioquímica y Microbiología, Valdivia, Chile.
- 2. Facultad de Medicina, Instituto de Microbiología Clínica, Valdivia, Los Ríos, Chile.
- 3. Center for Interdisciplinary Studies on the Nervous System (CISNe), Universidad Austral de Chile, Valdivia, Chile.
- 4. Facultad de Ciencias Veterinarias, Instituto de Farmacología, Universidad Austral de Chile, Valdivia, Los Ríos, Chile.
Interleukin-3 (IL-3) is a well-characterized growth factor in hematopoietic cells, but it is also expressed in Other cell types with poorly described functions. Many studies have provided evidence that IL-3 plays an important role in cell survival. We have previously shown that IL-3 is able to increase glucose uptake in HEK293 cells, suggesting that this factor requires sustained glucose metabolism to promote cell survival. In this study, we demonstrate that IL-3 contributes to cell survival under oxidative stress, a prominent feature in the pathophysiology of Cancer, diabetes, and neurodegenerative diseases, as well as in the aging process. Our results suggest a molecular mechanism that involves signaling pathways mediated by PI-3k/Akt and ERK. Altogether, these findings show an important role for IL-3 in supporting the viability of non-hematopoietic systems. J. Cell. Biochem. 118: 1330-1340, 2017. © 2016 Wiley Periodicals, Inc.