Application of modified yeast surface display technologies for non-Antibody protein engineering

  • Microbiol Res. 2017 Mar:196:118-128. doi: 10.1016/j.micres.2016.12.002.
Meng Mei  1 ,  Yu Zhou  1 ,  Wenfang Peng  1 ,  Chan Yu  1 ,  Lixin Ma  1 ,  Guimin Zhang  2 ,  Li Yi  3
Affiliations
  • 1. Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, 430062, China.
  • 2. Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, 430062, China. Electronic address: [email protected].
  • 3. Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, 430062, China. Electronic address: [email protected].
PMID: 28164786 DOI: 10.1016/j.micres.2016.12.002
Abstract

Yeast surface display (YSD) system has been widely used in protein engineering since it was established 20 years ago. Combined with fluorescence-activated cell sorting (FACS) technology and directed evolution, YSD has been proven of its extraordinary effectiveness for molecular engineering of various target proteins, especially for antibodies. Recently, a few remarkable efforts were exploited to modify the original Aga1-Aga2 YSD for the non-antibody protein engineering with successful outcomes, expanding its application on oxidase, Class II major histocompatibility complex (MHC-II), protease, sortase, lipoic acid Ligase etc. Here, the methodologies of these optimized Aga1-Aga2 YSD technologies were introduced, and the recent progress of non-antibody protein engineering using these methods was summarized.

Keywords
Directed evolution; FACS; Non-antibody protein engineering; Yeast surface display.
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